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Species: Homo sapiens
Genetic Insert: BRD9
Vector Backbone Description: Backbone Size:7656; Vector Backbone:MSCV-transgene-PGK-Puro IRES-GFP; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27376689
Proper citation: RRID:Addgene_75121 Copy
Species: Homo sapiens
Genetic Insert: KMT6
Vector Backbone Description: Backbone Size:7656; Vector Backbone:MSCV-transgene-PGK-Puro IRES-GFP; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27376689
Proper citation: RRID:Addgene_75125 Copy
Species: Homo sapiens
Genetic Insert: RAB5A
Vector Backbone Description: Vector Backbone:pCIG3; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_75111 Copy
Species: Homo sapiens
Genetic Insert: BRD9
Vector Backbone Description: Backbone Size:7656; Vector Backbone:MSCV-transgene-PGK-Puro IRES-GFP; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27376689
Proper citation: RRID:Addgene_75115 Copy
Species: Homo sapiens
Genetic Insert: PAX9
Vector Backbone Description: Vector Backbone:pMSCV-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17925434
Proper citation: RRID:Addgene_75079 Copy
Species: Homo sapiens
Genetic Insert: BRD9
Vector Backbone Description: Backbone Size:7656; Vector Backbone:MSCV-transgene-PGK-Puro IRES-GFP; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27376689
Proper citation: RRID:Addgene_75119 Copy
Species: Homo sapiens
Genetic Insert: NKX2-8
Vector Backbone Description: Vector Backbone:pMSCV-Zeocin; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17925434
Proper citation: RRID:Addgene_75090 Copy
Species: Homo sapiens
Genetic Insert: shBRD9_561
Vector Backbone Description: Vector Backbone:MSCV-miRE- shRNA-SV40-GFP; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27376689
Proper citation: RRID:Addgene_75139 Copy
Species: Homo sapiens
Genetic Insert: shBRD9_508
Vector Backbone Description: Vector Backbone:MSCV-miRE- shRNA-SV40-GFP; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27376689
Proper citation: RRID:Addgene_75138 Copy
Species: Homo sapiens
Genetic Insert: FADD sgRNA
Vector Backbone Description: Vector Backbone:lentiCRISPRv1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27194728
Proper citation: RRID:Addgene_75166 Copy
Species: Homo sapiens
Genetic Insert: RIP3 sgRNA
Vector Backbone Description: Vector Backbone:lentiCRISPRv1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27194728
Proper citation: RRID:Addgene_75165 Copy
Species: Homo sapiens
Genetic Insert: SLC24A2
Vector Backbone Description: Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17172467
Comments: FLAG tag is inserted internal to the protein coding region, as annotated in the annotated sequence file.
Proper citation: RRID:Addgene_75203 Copy
Species: Homo sapiens
Genetic Insert: SLC24A3
Vector Backbone Description: Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11294880
Comments: FLAG tag is inserted internal to the protein coding region, as annotated in the annotated sequence file.
Note, in addition to the flag tag, this plasmid differs from Plasmid 75205: Human NCKX3 (pcDNA3.1+) in that it includes a long 3' UTR sequence after the NCX3 ORF.
Proper citation: RRID:Addgene_75206 Copy
Species: Homo sapiens
Genetic Insert: Human Sacsin 1-339
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29945973
Proper citation: RRID:Addgene_75298 Copy
Species: Homo sapiens
Genetic Insert: SLC24A5
Vector Backbone Description: Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_75214 Copy
Species: Homo sapiens
Genetic Insert: SLC24A4
Vector Backbone Description: Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12379639
Comments: FLAG tag is inserted internal to the protein coding region, as annotated in the annotated sequence file.
Proper citation: RRID:Addgene_75212 Copy
Species: Homo sapiens
Genetic Insert: shRNA against hBcl10
Vector Backbone Description: Vector Backbone:pQCXIX (modified); Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27637333
Comments: Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
Produced 70-80 % KD (Western Blot) in Jurkat cells after RV-transduction and sort for GFPpositive cells.
Proper citation: RRID:Addgene_75248 Copy
Species: Homo sapiens
Genetic Insert: sgRNA against NFATc2
Vector Backbone Description: Backbone Marker:Feng Zhang (Plasmid #48138); Backbone Size:9703; Vector Backbone:PX458-pSpCas9(BB)2A-GFP; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27637333
Comments: gRNA contains at least 3 missmatches against human DNA (http://crispr.mit.edu). 2 additional nonmatching Guanidines added at 5' to enhnace specificy (Kim, D. et al. Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat. Methods 12, 237–43, 1 p following 243 (2015).)
targets all isoforms.
Proper citation: RRID:Addgene_75233 Copy
Species: Homo sapiens
Genetic Insert: sgRNA against NFATc1
Vector Backbone Description: Backbone Marker:Feng Zhang (Plasmid #48140); Backbone Size:9703; Vector Backbone:PX461-pSpCas9n(BB)2A-GFP; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27637333
Comments: gRNA contains at least 3 missmatches against human DNA (http://crispr.mit.edu). 2 additional nonmatching Guanidines added at 5' to enhnace specificy (Kim, D. et al. Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat. Methods 12, 237–43, 1 p following 243 (2015).)
targets all isoforms.
MUST BE COTRANSFECTED WITH PLASMID #75238
Proper citation: RRID:Addgene_75239 Copy
Species: Homo sapiens
Genetic Insert: sgRNA against NFATc1
Vector Backbone Description: Backbone Marker:Feng Zhang (Plasmid #48140); Backbone Size:9703; Vector Backbone:PX461-pSpCas9n(BB)2A-GFP; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27637333
Comments: gRNA contains at least 3 missmatches against human DNA (http://crispr.mit.edu). 2 additional nonmatching Guanidines added at 5' to enhnace specificy (Kim, D. et al. Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat. Methods 12, 237–43, 1 p following 243 (2015).)
targets all isoforms.
MUST BE COTRANSFECTED WITH PLASMID #75239
Proper citation: RRID:Addgene_75238 Copy
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