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Species: Homo sapiens
Genetic Insert: YAP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7800; Vector Backbone:pQCXIH; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17974916
Proper citation: RRID:Addgene_33091 Copy
Species: Homo sapiens
Genetic Insert: YAP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7800; Vector Backbone:pQCXIH; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17974916
Proper citation: RRID:Addgene_33092 Copy
Species: Homo sapiens
Genetic Insert: YAP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7800; Vector Backbone:pQCXIH; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20048001
Proper citation: RRID:Addgene_33068 Copy
Species: Homo sapiens
Genetic Insert: YAP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7800; Vector Backbone:pQCXIH; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20048001
Comments: From Depositor: S128A and S131A are also present due to alanine scanning mutagenesis. However, they are not important and do not alter plasmid function.
Proper citation: RRID:Addgene_33069 Copy
Species: E. coli
Genetic Insert: LacO (256 copies)
Vector Backbone Description: Vector Backbone:Ylplac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18614049
Comments: Addgene cannot verify or guarantee the presence of all 256 copies of LacO. This element is unstable.
For use in two-plasmid-based gene-nuclear periphery tethering assay with either plasmids pAK67 (Addgene #33142).
pVS2 (Addgene #33144) contains 256 LacO repeats and a GAL-GFP-GALpA-reporter gene (pVS1 is identical but does not contain the reporter gene). The reporter was previously characterized and contains a GFP open reading frame flanked by the GAL1 promoter and GAL1 3′ UTR ([Abruzzi et al., 2006] and [Dower et al., 2004]).
pKA67 expresses both LacI-GFP and Nup49-GFP to mark the first plasmid as well as the nuclear periphery with GFP.
Proper citation: RRID:Addgene_33143 Copy
Species: Homo sapiens
Genetic Insert: eIF4E
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21876152
Proper citation: RRID:Addgene_33252 Copy
Species: Drosophila melanogaster
Genetic Insert: Cyc
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pAc5.1/V5-His A; Vector Types:Insect Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18494558
Proper citation: RRID:Addgene_33156 Copy
Species: Drosophila melanogaster
Genetic Insert: CLK
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pAc5.1/V5-His B; Vector Types:Insect Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11158307
Proper citation: RRID:Addgene_33153 Copy
Species: Orthoreovirus T1L
Genetic Insert: L1
Vector Backbone Description: Backbone Marker:made by Yoshihiro Kawaoka; Vector Backbone:p3E5EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20042210
Proper citation: RRID:Addgene_33303 Copy
Species: Orthoreovirus T3D
Genetic Insert: S2
Vector Backbone Description: Backbone Marker:made by Yoshihiro Kawaoka; Vector Backbone:p3E5EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20042210
Proper citation: RRID:Addgene_33302 Copy
Species: uncultured proteobacterium EBAC31A08
Genetic Insert: Proteorhodopsin Optical Proton Sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4127; Vector Backbone:pBAD TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21764748
Proper citation: RRID:Addgene_33780 Copy
Species: firefly
Genetic Insert: Firefly Luciferase
Vector Backbone Description: Backbone Marker:David Baltimore; Vector Backbone:cFUGW; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21439256
Proper citation: RRID:Addgene_33307 Copy
Genetic Insert: HSV-TK/GFP
Vector Backbone Description: Vector Backbone:cFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19114988
Proper citation: RRID:Addgene_33308 Copy
Species: Homo sapiens
Genetic Insert: TCPTP-45
Vector Backbone Description: Backbone Marker:Vanberbilt structural biology core plasmid; Vector Backbone:pBG100; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20940300
Proper citation: RRID:Addgene_33365 Copy
Species: Homo sapiens
Genetic Insert: Cav1
Vector Backbone Description: Backbone Marker:GE Healthcare; Vector Backbone:pGEX-6p; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20940300
Proper citation: RRID:Addgene_33364 Copy
Genetic Insert: TetR cassette
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pCRII; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Comments: Do not select for this plasmid with tetracycline, as we have found that the Tet resistance cassette does not work reliably in high-copy plasmids such as this one. The Tet cassette MUST be transferred to a low-copy vector, e.g. a BAC, to allow Tet resistance in a reliable fashion. In BAC clones the Tet cassette has been extremely reliable.
Proper citation: RRID:Addgene_33359 Copy
Species: Mus musculus
Genetic Insert: Atoh1
Vector Backbone Description: Backbone Marker:Addgene plasmid #33336; Backbone Size:6575; Vector Backbone:pMSCV-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20516124
Proper citation: RRID:Addgene_33333 Copy
Species: Orthoreovirus T1L
Genetic Insert: S1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pBacPAK8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18005692
Proper citation: RRID:Addgene_33292 Copy
Species: Mus musculus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pRRLsin.PPT.hPGK.FLAG-CaMKKbeta-IRES.GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21669867
Comments: Please note that, according to NCBI numbering, the mutation is actually K194A.
This plasmid was also used in the following publication:
Cell Signal. 2011 Dec;23(12):2005-12. Epub 2011 Jul 23.
Proper citation: RRID:Addgene_33323 Copy
Species: Mus musculus
Genetic Insert: Malt1
Vector Backbone Description: Vector Backbone:pEF-FLAG-Strep; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21896478
Proper citation: RRID:Addgene_33315 Copy
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