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Species: Homo sapiens
Genetic Insert: OsTIR1(F74G)-P2A-mAID-EGFP-NES
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33177522
Proper citation: RRID:Addgene_140730 Copy
Species: Synthetic
Genetic Insert: Cas9m4-KRAB-MeCP2
Vector Backbone Description: Backbone Marker:Andrea Califano; Vector Backbone:PB-TRE-dCas9-KRAB-MeCP2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: This is a lentiviral vector (modified in #122205; originally from Weissman lab #85969) with a Tet-ON 3G dCas9m4-KRAB-MeCP2 (Church lab; #63800). Note: the vector has been modified to use blasticidin selection.
Proper citation: RRID:Addgene_140690 Copy
Species: Mus musculus
Genetic Insert: PI3K regulatory subunit p85beta
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Cantley lab recommends growth at room temperature.
Proper citation: RRID:Addgene_1408 Copy
Species: Synthetic
Genetic Insert: Lapronic (Lactate/PYRuvate Ratio Optical Nano-Indicator from CECs)
Vector Backbone Description: Backbone Size:5422; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32600029
Proper citation: RRID:Addgene_140756 Copy
Genetic Insert: Bacterial luciferase
Vector Backbone Description: Backbone Size:5681; Vector Backbone:pAKgfp1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17207855
Proper citation: RRID:Addgene_14083 Copy
Species: Homo sapiens
Genetic Insert: PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ]
Vector Backbone Description: Backbone Size:10065; Vector Backbone:pLX307; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31771968
Proper citation: RRID:Addgene_140941 Copy
Species: Synthetic
Genetic Insert: PuroTK
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Comments: Plate note: Plasmid contains a single G nucleotide deletion in the attL1 sequence. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_141005 Copy
Species: Ralstonia eutropha H16
Genetic Insert: phaCAB
Vector Backbone Description: Backbone Size:2883; Vector Backbone:pHB-2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32339760
Proper citation: RRID:Addgene_140957 Copy
Species: Synthetic
Genetic Insert: HygTK
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141006 Copy
Species: Escherichia coli
Genetic Insert: Neomycin resistance gene
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141007 Copy
Species: Homo sapiens
Genetic Insert: GAlphai3-RLuc8
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5189; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32367019
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_140975 Copy
Species: Homo sapiens
Genetic Insert: GAlphaQ-RLuc8
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5189; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32367019
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_140982 Copy
Species: Mus musculus
Genetic Insert: PI3K catalytic subunit p110alpha-Myc- (iSH2-p85alpha)
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pSRalpha; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please note that NcoI is not a unique enzyme and will cut the plasmid DNA at five locations.
Proper citation: RRID:Addgene_1410 Copy
Species: Synthetic
Genetic Insert: SpiCee-NLS
Vector Backbone Description: Vector Backbone:pCX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32697983
Proper citation: RRID:Addgene_140900 Copy
Species: Homo sapiens
Genetic Insert: GGamma1-GFP2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5432; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32367019
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_140989 Copy
Species: Synthetic
Genetic Insert: mutSpiCee
Vector Backbone Description: Vector Backbone:pCX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32697983
Proper citation: RRID:Addgene_140903 Copy
Species: Homo sapiens
Genetic Insert: GAlpha13-RLuc8
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5203; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32367019
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_140986 Copy
Species: Homo sapiens
Genetic Insert: GGamma9-GFP2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5432; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32367019
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_140991 Copy
Species: Synthetic
Genetic Insert: TetR-sfGFP expressed with T3 promoter
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30487584
Proper citation: RRID:Addgene_140871 Copy
Species: Discosoma
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:pMV361-strep; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:32829077
Comments: tdTomato: Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein
Shaner Nc, Campbell Re, Steinbach Pa, Giepmans Bng, Palmer Ae, Tsien Ry
(2004). Nature Biotechnology, 22(12) , 1567-1572. doi: 10.1038/nbt1037.
Proper citation: RRID:Addgene_140994 Copy
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