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Species: Synthetic
Genetic Insert: human codon usage NLS-anCas(PCA)-BPNLS-P2A-eGFP
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36593295
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.03.30.485982v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_185486 Copy
Species: Synthetic
Genetic Insert: human codon usage NLS-anCas(FCA)-BPNLS-P2A-eGFP
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36593295
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.03.30.485982v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_185487 Copy
Species: Synthetic
Genetic Insert: circular RNA-scaffolded sensing modules
Vector Backbone Description: Backbone Marker:Addgene plasmid #129405; Vector Backbone:pAV U6+27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34431301
Proper citation: RRID:Addgene_185406 Copy
Species: Synthetic
Genetic Insert: PPGK-driven GS-linker
Vector Backbone Description: Backbone Size:4435; Vector Backbone:pMM1 (pcDNA3.1-based); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33857582
Proper citation: RRID:Addgene_169481 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-MaCPNS1 VP1 gene
Vector Backbone Description: Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35643078
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. This work supported by NIH BRAIN Initiative grant UF1MH128336 . The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_185136 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-MaCPNS2 VP1 gene
Vector Backbone Description: Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35643078
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. This work supported by NIH BRAIN Initiative grant UF1MH128336 . The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_185137 Copy
Species: Synthetic
Genetic Insert: SNAPtag-LacI
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pSNAPf; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35537448
Proper citation: RRID:Addgene_183922 Copy
Species: Synthetic
Genetic Insert: MTRIA-NMB
Vector Backbone Description: Vector Backbone:pCIS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36138174
Comments: Please visit https://doi.org/10.1101/2021.07.30.454450 for bioRxiv preprint.
Proper citation: RRID:Addgene_184610 Copy
Species: Synthetic
Genetic Insert: PCMV-driven P2A-fused enhanced yellow fluorescent protein
Vector Backbone Description: Backbone Size:4435; Vector Backbone:pMM1 (pcDNA3.1-based); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33857582
Proper citation: RRID:Addgene_169526 Copy
Species: Synthetic
Genetic Insert: CYP19A1
Vector Backbone Description: Vector Backbone:CROPseq; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34890975
Proper citation: RRID:Addgene_183280 Copy
Species: Synthetic
Genetic Insert: PCMV-driven monomeric cyan fluorescent protein mTFP1
Vector Backbone Description: Backbone Size:4435; Vector Backbone:pMM1 (pcDNA3.1-based); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33857582
Proper citation: RRID:Addgene_169511 Copy
Species: Synthetic
Genetic Insert: PCMV-driven monomeric cyan fluorescent protein mTurquoise2
Vector Backbone Description: Backbone Size:4435; Vector Backbone:pMM1 (pcDNA3.1-based); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33857582
Proper citation: RRID:Addgene_169512 Copy
Species: Synthetic
Genetic Insert: ESR1
Vector Backbone Description: Vector Backbone:CROPseq; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34890975
Proper citation: RRID:Addgene_183288 Copy
Species: Synthetic
Genetic Insert: EPHA2
Vector Backbone Description: Vector Backbone:CROPseq; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34890975
Proper citation: RRID:Addgene_183286 Copy
Species: Synthetic
Genetic Insert: ERBB2
Vector Backbone Description: Vector Backbone:CROPseq; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34890975
Proper citation: RRID:Addgene_183287 Copy
Species: Synthetic
Genetic Insert: DNMT1
Vector Backbone Description: Vector Backbone:CROPseq; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34890975
Proper citation: RRID:Addgene_183282 Copy
Species: Synthetic
Genetic Insert: DNMT3A
Vector Backbone Description: Vector Backbone:CROPseq; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34890975
Proper citation: RRID:Addgene_183283 Copy
Species: Synthetic
Genetic Insert: PCMV-driven fluorescent timer, fast maturation time
Vector Backbone Description: Backbone Size:4435; Vector Backbone:pMM1 (pcDNA3.1-based); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33857582
Proper citation: RRID:Addgene_169518 Copy
Species: Synthetic
Genetic Insert: PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
Vector Backbone Description: Backbone Size:3092; Vector Backbone:pTS1019 (pUC57-based); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33857582
Proper citation: RRID:Addgene_169659 Copy
Species: Synthetic
Genetic Insert: PRPBSA-driven PuroR expression
Vector Backbone Description: Backbone Size:3092; Vector Backbone:pTS1019 (pUC57-based); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33857582
Proper citation: RRID:Addgene_169655 Copy
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