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Species: Staphylococcus aureus
Genetic Insert: sortase A pentamutant
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5370; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21697512
Comments: This plasmid has been found to be somewhat unstable and prone to multimerization. Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_75144 Copy
Species: Mus musculus
Genetic Insert: NIMA (never in mitosis gene a)-related expressed kinase 7
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26642356
Proper citation: RRID:Addgene_75142 Copy
Species: Staphylococcus aureus
Genetic Insert: reprogrammed sortase A variant
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5370; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25187567
Comments: eSrtA(4S-9) has 120 fold change in substrate specificity with activity comparable to eSrtA and it prefers LPXSG substrate over wild-type LPXTG. See sortase A pentamutant (eSrtA) in pET29 for original eSrtA (Addgene plasmid# 75144).
Proper citation: RRID:Addgene_75146 Copy
Species: Staphylococcus aureus
Genetic Insert: reprogrammed sortase A variant
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5370; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25187567
Comments: eSrtA(2A-9) has 51,000 fold change in substrate specificity with activity comparable to eSrtA and it prefers LAXTG substrate over wild-type LPXTG. See sortase A pentamutant (eSrtA) in pET29 for original eSrtA (Addgene plasmid# 75144).
Proper citation: RRID:Addgene_75145 Copy
Species: Synthetic
Genetic Insert: mTagBFP-CAAX
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:Middle entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin
Defining Citation: PMID:27631880
Proper citation: RRID:Addgene_75149 Copy
Species: Mus musculus
Genetic Insert: interleukin 1 beta
Vector Backbone Description: Backbone Size:6300; Vector Backbone:p3XFLAG-CMV-14; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26642356
Comments: Please note that mutation E130K in Il1b was found during Addgene's quality control. The depositor noted that this mutation is expected and does NOT affect its cleavage by caspase 1.
Proper citation: RRID:Addgene_75131 Copy
Species: Mus musculus
Genetic Insert: shBrd9_1116
Vector Backbone Description: Vector Backbone:MSCV-miR30-shRNA-PGK-NeoR-IRES-GFP; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27376689
Proper citation: RRID:Addgene_75130 Copy
Species: Mus musculus
Genetic Insert: NLR family, pyrin domain containing 3
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26642356
Proper citation: RRID:Addgene_75137 Copy
Species: Homo sapiens
Genetic Insert: shBRD9_508
Vector Backbone Description: Vector Backbone:MSCV-miRE- shRNA-SV40-GFP; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27376689
Proper citation: RRID:Addgene_75138 Copy
Vector Backbone Description: Vector Backbone:lentiCRISPRv1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27194728
Proper citation: RRID:Addgene_75162 Copy
Species: Homo sapiens
Genetic Insert: FADD sgRNA
Vector Backbone Description: Vector Backbone:lentiCRISPRv1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27194728
Proper citation: RRID:Addgene_75166 Copy
Species: Homo sapiens
Genetic Insert: SLC24A3
Vector Backbone Description: Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11294880
Comments: FLAG tag is inserted internal to the protein coding region, as annotated in the annotated sequence file.
Note, in addition to the flag tag, this plasmid differs from Plasmid 75205: Human NCKX3 (pcDNA3.1+) in that it includes a long 3' UTR sequence after the NCX3 ORF.
Proper citation: RRID:Addgene_75206 Copy
Species: Synthetic
Genetic Insert: mKOFP2-CAAX
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2565; Vector Backbone:pDONR221; Vector Types:Middle entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin
Defining Citation: PMID:27631880
Proper citation: RRID:Addgene_75155 Copy
Vector Backbone Description: Vector Backbone:lentiCRISPRv1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27194728
Proper citation: RRID:Addgene_75159 Copy
Species: Synthetic
Genetic Insert: H2B-mCerulean3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2533; Vector Backbone:pDONR221; Vector Types:Middle entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin
Defining Citation: PMID:27631880
Proper citation: RRID:Addgene_75156 Copy
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6648; Vector Backbone:pMAL-c2E; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20535468
Proper citation: RRID:Addgene_75291 Copy
Species: Homo sapiens
Genetic Insert: SLC24A5
Vector Backbone Description: Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_75214 Copy
Species: Xenopus laevis
Genetic Insert: H2BK120C
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4640; Vector Backbone:pET3a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26912860
Proper citation: RRID:Addgene_75332 Copy
Species: Other
Genetic Insert: TRE-DEST
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27133796
Proper citation: RRID:Addgene_75339 Copy
Species: Homo sapiens
Genetic Insert: SLC8B1
Vector Backbone Description: Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14625281
Proper citation: RRID:Addgene_75216 Copy
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