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Species: Drosophila melanogaster
Genetic Insert: CG1034
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22037703
Comments: Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
Proper citation: RRID:Addgene_34340 Copy
Genetic Insert: NLS-LSS-mKate2
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20212155
Comments: There is an 18bp deletion in Addgene's sequencing results relative to the full plasmid sequence (at position 2174-2192bp). This region is immediately after the LSS-mKate2 gene and is not critical for plasmid performance.
There is also a single nucleotide mismatch that results in an F22S change in the linker between the NLS and LSS-mKate2. It should not have any effect on the fusion performance.
Two other mismatches do not alter amino acid sequence.
Proper citation: RRID:Addgene_34586 Copy
Genetic Insert: tRNA^Sep
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Tetracycline
Defining Citation: PMID:21868676
Proper citation: RRID:Addgene_34624 Copy
Species: Methanocaldococcuc maripaludis
Genetic Insert: SepRS
Vector Backbone Description: Backbone Marker:Pharmacia; Backbone Size:4300; Vector Backbone:pKK223-3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21868676
Comments: To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which is catalyzing the last step in serine biosynthesis, was deleted from Escherichia coli strains Top10 (Top10∆serB - Addgene #34928) and BL21 (BL21∆serB - Addgene #34929). These strains are required hosts when using this plasmid.
Certain elements of this plasmid are covered under US patent 9,090,928 to Yale University
Proper citation: RRID:Addgene_34623 Copy
Species: Homo sapiens
Genetic Insert: KLF4
Vector Backbone Description: Backbone Marker:Morganstern JP and Land H 1990; Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21518959
Proper citation: RRID:Addgene_34589 Copy
Species: Rattus norvegicus
Genetic Insert: LAMP1
Vector Backbone Description: Backbone Size:7400; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22053050
Proper citation: RRID:Addgene_34611 Copy
Genetic Insert: Cre
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pMSCVhygro; Vector Types:Mammalian Expression, Retroviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20368440
Proper citation: RRID:Addgene_34565 Copy
Genetic Insert: HRV 3C-GPA-GFP-8HIS
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:5329; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20196076
Proper citation: RRID:Addgene_34562 Copy
Species: Homo sapiens
Genetic Insert: pLyn-CaRQ
Vector Backbone Description: Backbone Marker:PMID: 12864922 ; Backbone Size:5000; Vector Backbone:pTriEx-3; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22195563
Comments: LYN membrane anchor - MGCIKSKGKDSATS
MLCK peptide - KRRWKKNFIAVSAANRFKKI
IQ peptide - GSAITVQRYVRGIQARAYARFL
Proper citation: RRID:Addgene_34559 Copy
Species: Nostoc punctiforme
Genetic Insert: DnaE N-intein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3789; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20203672
Proper citation: RRID:Addgene_34549 Copy
Species: Homo sapiens
Genetic Insert: PRMT7
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX(SN); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15044439
Proper citation: RRID:Addgene_34693 Copy
Species: Homo sapiens
Genetic Insert: Sorting nexin 9
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17988218
Proper citation: RRID:Addgene_34690 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:Derived from pBluescript II SK+; Vector Types:Hydra Expression; Bacterial Resistance:Ampicillin
Comments: https://www.ncbi.nlm.nih.gov/nuccore/158426709
Proper citation: RRID:Addgene_34794 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-16
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBluescript; Vector Types:Bacterial Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12399591
Comments: Use HT115 bacteria for experiments--Grow O/N on LB supplemented with carbenicillin (100ug/ml) & tetracyclin (12.5ug/ml) and pick single colony for liquid culture.
Proper citation: RRID:Addgene_34833 Copy
Species: Homo sapiens
Genetic Insert: CD68 promoter/enhancer -680/+140
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11907098
Comments: The CD68 promoter/enhancer in this construct consists of ~680bp 5' of the ATG and the 83bp first intron of human CD68.
Please note that Addgene's sequencing results found a single nucleotide deletion at bp# 440 when compared to the full plasmid sequence provided by the depositor. The depositing scientist states that it is probably a polymorphism in the promoter and is not a concern for the function of the plasmid.
Proper citation: RRID:Addgene_34837 Copy
Species: Mus musculus
Genetic Insert: GATA3
Vector Backbone Description: Backbone Marker:Hergen Spits and coworkers; Backbone Size:12443; Vector Backbone:LZRSpBMN-linker-IRES-EGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12871641
Proper citation: RRID:Addgene_34836 Copy
Species: E. Coli
Genetic Insert: LplA W37V
Vector Backbone Description: Backbone Marker:Gift from John Cronan; Backbone Size:4800; Vector Backbone:pYFJ16; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20534555
Proper citation: RRID:Addgene_34838 Copy
Species: Homo sapiens
Genetic Insert: TFAM
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22037171
Proper citation: RRID:Addgene_34705 Copy
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Created by Köster and Fraser; Vector Backbone:act-GVP-UG; Vector Types:Xenopus expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22113462
Comments: The Sox2/Oct3-4 enhancer elements and a minimal promoter from the murine FGF4 were subcloned from the plasmid described in Figure 1 of Ambrosetti et al. (2000). The fragment contains six tandem repeats of the 35-bp fragment containing the Sox2/Oct3-4 heterodimer transcription factor binding domain (bd) followed by a 165-bp minimal FGF4 promoter (mFGF4). This plasmid was a gift of Claudio Basilico (New York University). To increase the FP signal, the Sox2/Oct 3-4-mFGF regulatory fragment was moved to the “act-GVP-UG” concatenated plasmid created by Köster and Fraser (2001), which was adapted from the Drosophila Gal4-UAS system (Brand and Perrimon,1993) and shown to function in Xenopus (Chae et al.,2002; Hartley et al.,2002). We replaced the minimal promoters of the act-GVP-UG plasmid with our Sox2/Oct3-mFGF regulatory fragment to control the expression of the transcriptional activator, Gal4-VP16, which in turn drives the expression of genes controlled by the upstream activating sequence (UAS).
The final expression vector (abbreviated as pSox2-bd::FP) contains: 6 repeats of the Sox2/Oct3-4 transcription factor binding domain, mFGF4, Gal4-VP16, a polyadenylation site, 14 repeats of UAS, mFGF4, and eGFP (ClonTech, Palo Alto, CA).
Sequencing with EGFP-N identified 1nt deletion at position 1733, 1 nt insertion at position 1214, 2 nt insertion at 1557 compared to the depositor provided sequence. These mutations are all in non-coding regions and have been shown by the depositing lab to not effect the function of the plasmid.
Proper citation: RRID:Addgene_34703 Copy
Species: Homo sapiens
Genetic Insert: NRF1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7000; Vector Backbone:pMSCV-hyg; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20385086
Proper citation: RRID:Addgene_34707 Copy
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