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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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sortase A pentamutant (eSrtA) in pET29 Resource Report Resource Website 10+ mentions |
RRID:Addgene_75144 | sortase A pentamutant | Staphylococcus aureus | Kanamycin | PMID:21697512 | This plasmid has been found to be somewhat unstable and prone to multimerization. Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid. | Backbone Marker:Novagen; Backbone Size:5370; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | P94R, D160N, D165A, K190E, K196T. Deleted amino acids 1-59 (which do not affect catalytic activity). | 2026-08-15 01:19:43 | 36 |
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pcDNA3-N-HA-NEK7 Resource Report Resource Website 10+ mentions |
RRID:Addgene_75142 | NIMA (never in mitosis gene a)-related expressed kinase 7 | Mus musculus | Ampicillin | PMID:26642356 | Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:19:43 | 10 | ||
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eSrtA(4S-9) pET29b Resource Report Resource Website 1+ mentions |
RRID:Addgene_75146 | reprogrammed sortase A variant | Staphylococcus aureus | Kanamycin | PMID:25187567 | eSrtA(4S-9) has 120 fold change in substrate specificity with activity comparable to eSrtA and it prefers LPXSG substrate over wild-type LPXTG. See sortase A pentamutant (eSrtA) in pET29 for original eSrtA (Addgene plasmid# 75144). | Backbone Marker:Novagen; Backbone Size:5370; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | N98D S102C A104V A118T F122A K134R F144L I182V E189F compared to eSrtA. Deleted amino acids 1-59 (which do not affect catalytic activity). | 2026-08-15 01:19:43 | 4 |
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eSrtA(2A-9) pET29b Resource Report Resource Website 1+ mentions |
RRID:Addgene_75145 | reprogrammed sortase A variant | Staphylococcus aureus | Kanamycin | PMID:25187567 | eSrtA(2A-9) has 51,000 fold change in substrate specificity with activity comparable to eSrtA and it prefers LAXTG substrate over wild-type LPXTG. See sortase A pentamutant (eSrtA) in pET29 for original eSrtA (Addgene plasmid# 75144). | Backbone Marker:Novagen; Backbone Size:5370; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | S102C A104H E105D K138P K152I N160K K162H T164N K173E I182V T196S compared to eSrtA. Deleted amino acids 1-59 (which do not affect catalytic activity). | 2026-08-15 01:19:43 | 4 |
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pME-mTagBFP-CAAX Resource Report Resource Website 1+ mentions |
RRID:Addgene_75149 | mTagBFP-CAAX | Synthetic | Kanamycin | PMID:27631880 | Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:Middle entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin | 2026-08-15 01:19:43 | 4 | ||
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pCMV-pro-Il1b-C-Flag Resource Report Resource Website 1+ mentions |
RRID:Addgene_75131 | interleukin 1 beta | Mus musculus | Ampicillin | PMID:26642356 | Please note that mutation E130K in Il1b was found during Addgene's quality control. The depositor noted that this mutation is expected and does NOT affect its cleavage by caspase 1. | Backbone Size:6300; Vector Backbone:p3XFLAG-CMV-14; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | (please see depositor comments below) | 2026-08-15 01:19:43 | 6 |
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MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_75130 | shBrd9_1116 | Mus musculus | Ampicillin | PMID:27376689 | Vector Backbone:MSCV-miR30-shRNA-PGK-NeoR-IRES-GFP; Vector Types:Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:19:43 | 1 | ||
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pcDNA3-N-Flag-NLRP3 1-90 Resource Report Resource Website 1+ mentions |
RRID:Addgene_75137 | NLR family, pyrin domain containing 3 | Mus musculus | Ampicillin | PMID:26642356 | Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | fragment encoding amino acids 1-90 | 2026-08-15 01:19:43 | 2 | |
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MSCV-miRE_shBRD9_508-SV40-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_75138 | shBRD9_508 | Homo sapiens | Ampicillin | PMID:27376689 | Vector Backbone:MSCV-miRE- shRNA-SV40-GFP; Vector Types:Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:19:43 | 1 | ||
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pLentiCRISPR-RFP657 Resource Report Resource Website 1+ mentions |
RRID:Addgene_75162 | Ampicillin | PMID:27194728 | Vector Backbone:lentiCRISPRv1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:19:43 | 5 | ||||
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pLC-BFP-FADD Resource Report Resource Website 1+ mentions |
RRID:Addgene_75166 | FADD sgRNA | Homo sapiens | Ampicillin | PMID:27194728 | Vector Backbone:lentiCRISPRv1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:19:43 | 1 | ||
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Human NCKX3-FlagTag (pcDNA3.1-) Resource Report Resource Website 1+ mentions |
RRID:Addgene_75206 | SLC24A3 | Homo sapiens | Ampicillin | PMID:11294880 | FLAG tag is inserted internal to the protein coding region, as annotated in the annotated sequence file. Note, in addition to the flag tag, this plasmid differs from Plasmid 75205: Human NCKX3 (pcDNA3.1+) in that it includes a long 3' UTR sequence after the NCX3 ORF. | Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:19:43 | 2 | |
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pME-mKOFP2-CAAX Resource Report Resource Website 1+ mentions |
RRID:Addgene_75155 | mKOFP2-CAAX | Synthetic | Kanamycin | PMID:27631880 | Backbone Marker:Invitrogen; Backbone Size:2565; Vector Backbone:pDONR221; Vector Types:Middle entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin | 2026-08-15 01:19:43 | 1 | ||
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pLentiCRISPR-EGFP Resource Report Resource Website 10+ mentions |
RRID:Addgene_75159 | Ampicillin | PMID:27194728 | Vector Backbone:lentiCRISPRv1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:19:43 | 15 | ||||
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pME-H2B-mCerulean3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_75156 | H2B-mCerulean3 | Synthetic | Kanamycin | PMID:27631880 | Backbone Marker:Invitrogen; Backbone Size:2533; Vector Backbone:pDONR221; Vector Types:Middle entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin | 2026-08-15 01:19:43 | 1 | ||
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pMAL-c2E Resource Report Resource Website 1+ mentions |
RRID:Addgene_75291 | Ampicillin | PMID:20535468 | Backbone Marker:New England Biolabs; Backbone Size:6648; Vector Backbone:pMAL-c2E; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:19:44 | 1 | ||||
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Human NCKX5 (pcDNA3.1+) Resource Report Resource Website 1+ mentions |
RRID:Addgene_75214 | SLC24A5 | Homo sapiens | Ampicillin | Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:19:43 | 1 | |||
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H2BK120C Resource Report Resource Website 1+ mentions |
RRID:Addgene_75332 | H2BK120C | Xenopus laevis | Ampicillin | PMID:26912860 | Backbone Marker:Novagen; Backbone Size:4640; Vector Backbone:pET3a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | truncated; K120C | 2026-08-15 01:19:44 | 2 | |
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Hygro-iDEST Resource Report Resource Website 1+ mentions |
RRID:Addgene_75339 | TRE-DEST | Other | Chloramphenicol and Ampicillin | PMID:27133796 | Vector Backbone:N/A; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:19:45 | 1 | ||
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Human NCKX6 (pcDNA3.1+) Resource Report Resource Website 1+ mentions |
RRID:Addgene_75216 | SLC8B1 | Homo sapiens | Ampicillin | PMID:14625281 | Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:19:43 | 2 |
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