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Species: Danio rerio
Genetic Insert: Rab33bb
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_140882 Copy
Species: simian virus
Genetic Insert: Large T antigen
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12781366
Proper citation: RRID:Addgene_14088 Copy
Species: Mus musculus
Genetic Insert: p19 ARF shRNA -2
Vector Backbone Description: Backbone Marker:Available from Addgene (#11795); Backbone Size:8000; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12853964
Comments: p19 ARF shRNA expressed from a lentiviral vector. The p19 ARF shRNA together with the H1 promoter was cloned from pSIRIP-puro into pLL3.7. Therefore, although the shRNA is normally expressed from the U6 promoter of pLL3.7, p19-ARF shRNA is expressed here from the H1 promoter.
Proper citation: RRID:Addgene_14091 Copy
Species: Homo sapiens
Genetic Insert: HMETTL14
Vector Backbone Description: Vector Backbone:pCDH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29290617
Proper citation: RRID:Addgene_141112 Copy
Species: Homo sapiens
Genetic Insert: HMETTL14
Vector Backbone Description: Vector Backbone:PCDH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29290617
Proper citation: RRID:Addgene_141113 Copy
Species: Homo sapiens
Genetic Insert: GCN5
Vector Backbone Description: Backbone Size:25000; Vector Backbone:pAdEasy; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16753578
Comments: Flag-GCN5 was cloned into the pAdTrack shuttle vector and adenovirus constructs were created by recombination of the shuttle vector and pAdEasy vector by electroporation into BJ5183-AD-1 bacteria (Stratagene).
AdEasy is a registered trademark of the Johns Hopkins University.
Proper citation: RRID:Addgene_14106 Copy
Vector Backbone Description: Backbone Size:6734; Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508591
Comments: Intent is to introduce low doses of pCAG FlpE to recombine a subset of cells, such that mNeon can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. Genetic overexpression can be easily attached following the mScarlet through use of a mcs after the P2A.
Please visit https://www.biorxiv.org/content/10.1101/571323v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141111 Copy
Species: Homo sapiens
Genetic Insert: NFAT5
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12080349
Comments: DNA binding domain mutant, dominant negative mutant. Also called DBD5.
Proper citation: RRID:Addgene_14112 Copy
Genetic Insert: zCas9(H840A)-OsM-MLV-RT
Vector Backbone Description: Vector Backbone:pYPQ165; Vector Types:Plant Expression, CRISPR, Gateway compatible zCas9(H840A)-OsM-MLV-RT; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32222487
Comments: Protein sequence of engineered M-MLV reverse transcriptase (D200N, L603W, T306K, W313F, T330P) was obtained from published work: Anzalone, A.V., Randolph, P.B., Davis, J.R., Sousa, A.A., Koblan, L.W., Levy, J.M., Chen, P.J., Wilson, C., Newby, G.A., Raguram, A. and Liu, D.R., 2019. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature, 576(7785), pp.149-157.
Proper citation: RRID:Addgene_141080 Copy
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:31454364
Comments: Please note: Plasmid contains a T to C nucleotide mutation in attP1. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_141014 Copy
Species: S. pyogenes
Genetic Insert: Cas9 and tracrRNA
Vector Backbone Description: Vector Backbone:colE1, pAM beta; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
Defining Citation: PMID:30156038
Proper citation: RRID:Addgene_141095 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:4416; Vector Backbone:pPEPZ; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33120116
Proper citation: RRID:Addgene_141090 Copy
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141017 Copy
Species: Synthetic
Genetic Insert: mVenus
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Comments: Please note: Plasmid contains a K206A mutation in Venus. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_141019 Copy
Vector Backbone Description: Backbone Size:15524; Vector Backbone:entiSAM v2 (Puro) addgene#92062; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_141104 Copy
Species: Homo sapiens
Genetic Insert: ZNF598
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5078; Vector Backbone:pcDNA4/TO/GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30917308
Proper citation: RRID:Addgene_141191 Copy
Species: Rattus norvegicus
Genetic Insert: Zdk2, Cofilin, LOV2
Vector Backbone Description: Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31740825
Proper citation: RRID:Addgene_141137 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP4
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_141258 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP5
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_141259 Copy
Species: Other
Genetic Insert: loxp-DsRed-loxp-eGFP
Vector Backbone Description: Backbone Marker:Eric Campeau & Paul Kaufman (Addgene plasmid # 17294); Vector Backbone:pLenti CMV/TO Zeo DEST (644-1) ; Vector Types:Lentiviral; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:31649238
Comments: loxp-DsRed-loxp-eGFP was PCR amplified from pMSCV-loxp-DsRed-loxp-eGFP-Puro-WPRE (Addgene plasmid #32702) using primers containing Sal1 and Not1 restriction enzyme sites. The amplified PCR fragment was digested with Sal1 and Not1 and ligated into the pENTRA1 vector cut with Sal1 and Not1. The Gateway SystemTM (Invitrogen) was used to recombine the pENTR1A shuttle vector with pLenti CMV/TO Zeo DEST (644-1) (#17294, Addgene) generating lentiviral vector 1.
Proper citation: RRID:Addgene_141148 Copy
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