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Species: Other
Genetic Insert: SARS-CoV S (spike)
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32504802
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166012 Copy
Species: Other
Genetic Insert: SARS-CoV-2 RBD
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33248226
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166019 Copy
Species: Other
Genetic Insert: HCoV-229E S (spike)
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32504802
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166016 Copy
Species: Other
Genetic Insert: SARS-CoV-2 S (spike)
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32504802
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166010 Copy
Species: Synthetic
Genetic Insert: tetR
Vector Backbone Description: Vector Backbone:pGK401red; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33758180
Comments: The synthetic transcription activator (rtTA) is originated from Tet systems (pTet-On Advanced, Tet systems).
Proper citation: RRID:Addgene_165968 Copy
Species: Synthetic
Genetic Insert: tetR
Vector Backbone Description: Vector Backbone:pGK415; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33758180
Comments: The synthetic transcription activator (rtTA) is originated from Tet systems (pTet-On Advanced, Tet systems).
Proper citation: RRID:Addgene_165969 Copy
Species: Synthetic
Genetic Insert: rShHTL7 cPGFP(D165)
Vector Backbone Description: Backbone Size:5717; Vector Backbone:pET19b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32786922
Proper citation: RRID:Addgene_166003 Copy
Species: Synthetic
Genetic Insert: rDAD2 cPGFP(N180)
Vector Backbone Description: Backbone Size:5717; Vector Backbone:pET19b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32786922
Comments: Depositor confirms mutation does not affect the expression of mOrange.
Proper citation: RRID:Addgene_166001 Copy
Species: Vibrio fischeri
Genetic Insert: luxR
Vector Backbone Description: Vector Backbone:pGK415; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33758180
Proper citation: RRID:Addgene_165971 Copy
Species: Xanthophyllomyces dendrorhous
Genetic Insert: Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33758180
Comments: A portion of this plasmid (the operator region of ptetO7) was derived from a plasmid (pTRE-Tight, Tet systems).
Proper citation: RRID:Addgene_165976 Copy
Species: Xanthophyllomyces dendrorhous
Genetic Insert: Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33758180
Proper citation: RRID:Addgene_165977 Copy
Vector Backbone Description: Backbone Size:4699; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD130.07, Ligation number L4507.
Proper citation: RRID:Addgene_1659 Copy
Species: Synthetic
Genetic Insert: DAD2 cPGFP(G164)
Vector Backbone Description: Backbone Size:5717; Vector Backbone:pET19b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32786922
Proper citation: RRID:Addgene_165993 Copy
Species: Synthetic
Genetic Insert: SpyCatcher-M2e
Vector Backbone Description: Backbone Size:5676; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33468139
Comments: Original publications:
SpyCatcher:
Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M. Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. doi: 10.1073/pnas.1115485109. Epub 2012 Feb 24. PMID: 22366317; PMCID: PMC3311370.
Proper citation: RRID:Addgene_165990 Copy
Species: Synthetic
Genetic Insert: HisDAD2
Vector Backbone Description: Backbone Size:5717; Vector Backbone:pET19b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32786922
Proper citation: RRID:Addgene_165996 Copy
Species: Synthetic
Genetic Insert: HisDAD2 cPGFP(G164)
Vector Backbone Description: Backbone Size:5717; Vector Backbone:pET19b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32786922
Proper citation: RRID:Addgene_165997 Copy
Species: Synthetic
Genetic Insert: rDAD2 cPGFP(G164)
Vector Backbone Description: Backbone Size:5717; Vector Backbone:pET19b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32786922
Proper citation: RRID:Addgene_165994 Copy
Species: Synthetic
Genetic Insert: rShHTL7 cPGFP(L166)
Vector Backbone Description: Backbone Size:5717; Vector Backbone:pET19b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32786922
Proper citation: RRID:Addgene_165995 Copy
Species: Synthetic
Genetic Insert: DAD2 cPGFP(A149)
Vector Backbone Description: Backbone Size:5717; Vector Backbone:pET19b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32786922
Proper citation: RRID:Addgene_165998 Copy
Species: Synthetic; S pyogenes Cas9
Genetic Insert: Cas9-D10A
Vector Backbone Description: Backbone Marker:Addgene 43804; Vector Backbone:p415; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_166095 Copy
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