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Species: Drosophila melanogaster
Genetic Insert: DmGW182_744-840
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147017 Copy
Species: Drosophila melanogaster
Genetic Insert: DmGW182_700-840
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147015 Copy
Species: Homo sapiens
Genetic Insert: HsPAN3_291-687
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: ORF exrtacted from NCBI:BC128180.1 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147013 Copy
Species: Homo sapiens
Genetic Insert: HsNot6
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: sequence extracted from NCBI: NM_015455.3 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147011 Copy
Species: Homo sapiens
Genetic Insert: HsXRN1_1597-1706
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: sequence given by NCBI : NM_019001.3. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147095 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Backbone Marker:Víctor de Lorenzo; Backbone Size:3730; Vector Backbone:pSEVA221; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36511859
Proper citation: RRID:Addgene_188965 Copy
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK090; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37930278
Comments: RFP expression in E. coli. Plasmid yields may drop after multiple passages or delayed miniprepping after the culture has saturated. We suggest streaking onto solid media to pick fresh colonies every time.
Proper citation: RRID:Addgene_180729 Copy
Species: Synthetic
Genetic Insert: 50%GC-40bp-hairpin-binding-site-reversed
Vector Backbone Description: Backbone Size:3956; Vector Backbone:pCR-XL-2-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region.
Proper citation: RRID:Addgene_169198 Copy
Species: Synthetic
Genetic Insert: 66%GC-20bp-hairpin
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region.
Proper citation: RRID:Addgene_169199 Copy
Species: Synthetic
Genetic Insert: 50%GC-20bp-hairpin
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region.
Proper citation: RRID:Addgene_169196 Copy
Species: Synthetic
Genetic Insert: 50%GC-40bp-hairpin
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region.
Proper citation: RRID:Addgene_169197 Copy
Species: Synthetic
Genetic Insert: 50-0-50%GC-Right-half-120bp-hairpin
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Since the 120nt hairpin cannot be cloned in it's entirety the two halves (Left & Right) are provided here.
Proper citation: RRID:Addgene_169207 Copy
Species: Synthetic
Genetic Insert: 100%GC-20bp-hairpin
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region.
Proper citation: RRID:Addgene_169203 Copy
Species: Synthetic
Genetic Insert: 50%GC-Right-half-120bp-hairpin
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Since the 120nt hairpin cannot be cloned in it's entirety the two halves (Left & Right) are provided here.
Proper citation: RRID:Addgene_169205 Copy
Species: Synthetic
Genetic Insert: 75%GC-20bp-hairpin
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region.
Proper citation: RRID:Addgene_169201 Copy
Species: Synthetic
Genetic Insert: ABE7.10-SpCas9-NG
Vector Backbone Description: Backbone Marker:Atum; Backbone Size:2256; Vector Backbone:pD881-SR; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34520545
Proper citation: RRID:Addgene_170663 Copy
Genetic Insert: T7-LacO-TetR-T7 Stop
Vector Backbone Description: Backbone Size:1986; Vector Backbone:T7-LacO-QF_p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34226549
Proper citation: RRID:Addgene_171664 Copy
Genetic Insert: QF-T7-TetO-TetR
Vector Backbone Description: Backbone Size:3062; Vector Backbone:T7-LacO-QF_p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34226549
Proper citation: RRID:Addgene_171661 Copy
Genetic Insert: T7-LacO-TetR-T7 Stop
Vector Backbone Description: Backbone Size:3192; Vector Backbone:T7-TetO-QF_p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34226549
Proper citation: RRID:Addgene_171659 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Size:6497; Vector Backbone:pRC; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35851375
Proper citation: RRID:Addgene_188115 Copy
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