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Species: Mus musculus
Genetic Insert: RAG-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4356; Vector Backbone:pCDM8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:8284210
Comments: cDNA was synthesized by reverse transcription of total RNA obtained from cell line 22D6, an Abelson murine leukemia virus transformed pre-B cell line. RAG-1 cDNA was then amplified by PCR, using specific primers designed also to add restriction enzyme recognition sites adjacent to the largest open reading frame for subsequent cloning. PCR products were subcloned into the pCDM8 vector.
Proper citation: RRID:Addgene_13328 Copy
Species: Mus musculus
Genetic Insert: Upstream region of mouse Fbx15 gene
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pGV-BM2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12665572
Comments: The sequence of primer pGV-2U is ACT AAC ATA CGC TCT CCA TCA A.
Proper citation: RRID:Addgene_13412 Copy
Species: Mus musculus
Genetic Insert: Gsdmd-NT
Vector Backbone Description: Vector Backbone:pFlag-CMV-4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27383986
Proper citation: RRID:Addgene_133890 Copy
Species: Mus musculus
Genetic Insert: Gsdmd-NT
Vector Backbone Description: Vector Backbone:pFlag-CMV-4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27383986
Proper citation: RRID:Addgene_133889 Copy
Species: Mus musculus
Genetic Insert: Gsdmd-NT
Vector Backbone Description: Vector Backbone:pFlag-CMV-4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27383986
Proper citation: RRID:Addgene_133888 Copy
Species: Mus musculus
Genetic Insert: MKP-1ASA
Vector Backbone Description: Backbone Size:5800; Vector Backbone:pWay21 EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15899879
Proper citation: RRID:Addgene_13479 Copy
Species: Mus musculus
Genetic Insert: PCAF
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20422051
Proper citation: RRID:Addgene_134572 Copy
Species: Mus musculus
Genetic Insert: GCN5
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20422051
Proper citation: RRID:Addgene_134573 Copy
Species: Mus musculus
Genetic Insert: KIF3C
Vector Backbone Description: Vector Backbone:pBa-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31461551
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
Proper citation: RRID:Addgene_134616 Copy
Species: Mus musculus
Genetic Insert: KIF1Balpha
Vector Backbone Description: Vector Backbone:pBa-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31461551
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
Proper citation: RRID:Addgene_134619 Copy
Species: Mus musculus
Genetic Insert: Sox2
Vector Backbone Description: Backbone Size:6800; Vector Backbone:pCAG-HA-gw-IP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15863505
Comments: The Sox2 cDNA contains a modified ATG start codon to TTG for cloning into vectors with an N-terminal tag.
Proper citation: RRID:Addgene_13459 Copy
Species: Mus musculus
Genetic Insert: Cyp26a1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5-HS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16818722
Comments: Full-length cDNA for mouse Cyp26a1 cloned into modified pSG5. See Author's map for more information.
Proper citation: RRID:Addgene_13457 Copy
Species: Mus musculus
Genetic Insert: KIF1Bbeta
Vector Backbone Description: Vector Backbone:pBa-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31461551
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
Addgene NGS identified the following amino acid discrepancies relative to Genbank accession: NP_997565.2: V406A, L536I, S542N, Y939C, Q1093R, T1187I, S1766N. The depositing laboratory has confirmed that the plasmid should function as described in their publication.
Proper citation: RRID:Addgene_134620 Copy
Species: Mus musculus
Genetic Insert: KIF5B
Vector Backbone Description: Vector Backbone:pBa-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31461551
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
Proper citation: RRID:Addgene_134625 Copy
Species: Mus musculus
Genetic Insert: KLC1a
Vector Backbone Description: Vector Backbone: pBa-FRB-3myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31461551
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
Proper citation: RRID:Addgene_134627 Copy
Species: Mus musculus
Genetic Insert: MKP-1 FL
Vector Backbone Description: Backbone Size:5800; Vector Backbone:pWay21 EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15899879
Proper citation: RRID:Addgene_13469 Copy
Species: Mus musculus
Genetic Insert: Oct3/4
Vector Backbone Description: Backbone Size:6900; Vector Backbone:pCAG-Myc-gw-IP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15863505
Proper citation: RRID:Addgene_13460 Copy
Species: Mus musculus
Genetic Insert: H2-Ld
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10381; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31796585
Proper citation: RRID:Addgene_135519 Copy
Species: Mus musculus
Genetic Insert: Myo 1g
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4701; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19968988
Proper citation: RRID:Addgene_134991 Copy
Species: Mus musculus
Genetic Insert: PI3K catalytic subunit p110alpha-Myc- (iSH2-p85alpha)
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pSRalpha; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please note that NcoI is not a unique enzyme and will cut the plasmid DNA at five locations.
Proper citation: RRID:Addgene_1410 Copy
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