Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Genetic Insert: TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI
Vector Backbone Description: Vector Backbone:pYPQ166; Vector Types:Plant Expression, CRISPR, Gateway compatible TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI entry clone; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38877035
Comments: Please visit https://doi.org/10.1101/2024.02.25.581917 for bioRxiv preprint.
Proper citation: RRID:Addgene_213470 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pLEU; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38906116
Comments: Contains a minimal CYC1 promoter driving expression of mNeonGreen. Binding sites for ZF-1 (ZF 43-8) are upstream of the promoter. Cut with NotI prior to transformation into yeast to facilitate genomic insertion.
** This plasmid is prone to recombination due to ZF binding site repeats.**
Proper citation: RRID:Addgene_213840 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pLEU; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38906116
Comments: Contains a minimal CYC1 promoter driving expression of mNeonGreen. Binding sites for ZF-2 (ZF 42-10) are upstream of the promoter. Cut with NotI prior to transformation into yeast to facilitate genomic insertion.
**This plasmid is prone to recombination due to ZF binding site repeats.**
Proper citation: RRID:Addgene_213841 Copy
Species: Synthetic
Genetic Insert: VP16
Vector Backbone Description: Vector Backbone:self-made; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38906116
Comments: Contains one aTc-inducible promoter variants driving the expression of VP-16 fused to ZF-1 (ZF-43-8).
**This plasmid is prone to recombination due to repeats in the aTc-inducible promoters.**
Proper citation: RRID:Addgene_213843 Copy
Vector Backbone Description: Vector Backbone:self-made; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38906116
Comments: Storage plasmid for type 1 expression vectors -- i.e., fusions to ZF-1 (ZF 43-8).
To insert barcoded genes:
i) digest this plasmid with BbsI and MfeI
ii) digest the barcoded gene with BsaI -- making sure the resultant overhangs match the BbsI and MfeI sites
iii) ligate the two digestion products.
Terminators can be added to the barcoded ligation product via a BbsI golden gate reaction.
Proper citation: RRID:Addgene_213844 Copy
Vector Backbone Description: Vector Backbone:self-made; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38906116
Comments: Storage plasmid for type 1 expression vectors -- i.e., fusions to ZF-2 (ZF 42-10).
To insert barcoded genes:
i) digest this plasmid with BbsI and MfeI
ii) digest the barcoded gene with BsaI -- making sure the resultant overhangs match the BbsI and MfeI sites
iii) ligate the two digestion products.
Terminators can be added to the barcoded ligation product via a BbsI golden gate reaction.
Proper citation: RRID:Addgene_213845 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pLEU; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38906116
Comments: Contains a minimal CYC1 promoter driving expression of mNeonGreen. No ZF binding sites are present.
Cut with NotI prior to transformation into yeast to facilitate genomic insertion.
Proper citation: RRID:Addgene_213838 Copy
Species: Synthetic
Genetic Insert: mNeonGreen, mCherry
Vector Backbone Description: Vector Backbone:self-made; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38906116
Comments: Contains two aTc-inducible variants driving the expression of mNeonGreen and mCherry.
**This plasmid is prone to recombination due to repeats in the aTc-inducible promoters.**
Proper citation: RRID:Addgene_213839 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pLEU; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38906116
Comments: Contains a minimal CYC1 promoter driving expression of mNeonGreen. Binding sites for ZF-1 (ZF 43-8) are upstream of the promoter and ZF-2 (ZF 42-10) binding sites are downstream.
Cut with NotI prior to transformation into yeast to facilitate genomic insertion.
**This plasmid is prone to recombination due to ZF binding site repeats.**
Proper citation: RRID:Addgene_213835 Copy
Species: Other
Genetic Insert: KDEL
Vector Backbone Description: Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_215002 Copy
Species: Rattus norvegicus
Genetic Insert: MAP1LC3B
Vector Backbone Description: Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_215004 Copy
Species: Synthetic
Genetic Insert: SF-LUZ-H3H-EGFP-p19
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega2; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37882352
Proper citation: RRID:Addgene_215257 Copy
Species: Synthetic
Genetic Insert: MAR10-SF-nptII-LUZ-CPH-MAR10-SF
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37882352
Proper citation: RRID:Addgene_215270 Copy
Species: Synthetic
Genetic Insert: MGGAGM Linker
Vector Backbone Description: Vector Backbone:pGWB502-omega; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39100877
Comments: Please visit https://www.biorxiv.org/content/10.1101/2024.06.28.601222v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_216141 Copy
Species: Arabidopsis thaliana
Genetic Insert: MPK12
Vector Backbone Description: Vector Backbone:pGWB502-omega; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39100877
Comments: Please visit https://www.biorxiv.org/content/10.1101/2024.06.28.601222v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_216139 Copy
Species: Arabidopsis thaliana
Genetic Insert: MPK5
Vector Backbone Description: Vector Backbone:pGWB502-omega; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39100877
Comments: Addgene NGS results found E483G mutation in insert ORF that does not impact plasmid function.
Please visit https://www.biorxiv.org/content/10.1101/2024.06.28.601222v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_216136 Copy
Species: Homo sapiens
Genetic Insert: STXBP1
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:2800; Vector Backbone:pCR8-GW-TOPO; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Comments: insert contains a Kozak sequence that encodes a linker for cloning into N-terminal tagging pDEST vectors
Proper citation: RRID:Addgene_216624 Copy
Species: Homo sapiens
Genetic Insert: YAP1 WT
Vector Backbone Description: Vector Backbone:pDONR; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35170430
Proper citation: RRID:Addgene_184524 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:CloCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33834762
Comments: Please visit https://www.biorxiv.org/content/10.1101/529180v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_139134 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:CloCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33834762
Comments: Please visit https://www.biorxiv.org/content/10.1101/529180v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_139137 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the RRID Resources search. From here you can search through a compilation of resources used by RRID and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that RRID has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on RRID then you can log in from here to get additional features in RRID such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into RRID you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within RRID that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
