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Genetic Insert: MpIGPDm and mALS
Vector Backbone Description: Vector Backbone:pMpGWB303; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37283617
Proper citation: RRID:Addgene_188555 Copy
Genetic Insert: MpIGPDm
Vector Backbone Description: Vector Backbone:pMpGWBhis03-Citrine; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37283617
Proper citation: RRID:Addgene_188556 Copy
Species: Candidatus Methanomethylophilus alvus
Genetic Insert: Pyrrolysyl-tRNA synthetase
Vector Backbone Description: Backbone Marker:Peter Schultz; Backbone Size:4029; Vector Backbone:pUltra; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37264106
Proper citation: RRID:Addgene_200226 Copy
Species: Synthetic
Genetic Insert: copper-inducible dCasEV2.1
Vector Backbone Description: Vector Backbone:pLX-B3_omega1; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37032497
Comments: Contains the Transcriptional Units for constitutive expression of hygromycin resistance and CUP2:GAL4, and for copper-inducible expression of dCas9:EDLL and MS2:VPR (dCasEV2.1)
Proper citation: RRID:Addgene_187810 Copy
Species: Synthetic
Genetic Insert: Moth Pheromone pathway
Vector Backbone Description: Vector Backbone:pDGB3_omega1; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37032497
Comments: Contains the Transcriptional Units for constitutive expression of kanamycin resistance, and for dCasEV2.1-regulated expression of Atr delta11, HarFAR and SpATF1-2
Proper citation: RRID:Addgene_187813 Copy
Species: Epstein-Barr virus
Genetic Insert: BLRF1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2875; Vector Backbone:pENTR223.1; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17446270
Proper citation: RRID:Addgene_195771 Copy
Species: Streptomyces rubellomurinus
Genetic Insert: FrbA
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37122473
Comments: The pCDF-Frb-v1 plasmid can be propagated reliably in DH10b cells, but it is good practice to minimize the number of propagations. Upon receiving cells and growing a colony overnight in liquid culture, make a frozen glycerol stock of these Dh10b cells containing plasmid. This glycerol stock can be used to inoculate future cultures for isolating more plasmid.
Glycerol stocks can be made by mixing 600 uL of overnight culture with 400 uL of 50% (v/v) sterile glycerol and placing tube in a -80C freezer.
This plasmid should be paired with pERM2-nhpSer (#201922, which enables translational encoding of the biosynthesized non-hydrolyzable phosphoserine amino acid) and a compatible plasmid expressing protein of interest (e.g. #174076 for a sfGFP-150TAG control) in the cell line BL21(DE3) deltaSerC (#197656). This system is used to translationally incorporate non-hydrolyzable phosphoserine into target proteins at TAG amber stop codons in E coli.
This plasmid exists as a dimer. This is not expected to impact the end function of the plasmid and the depositor recommends that the plasmid should be used exactly as provided. However, please refer to the Addgene Blog post on multimers for more information: https://blog.addgene.org/plasmids-101-dimers-and-multimers
Additionally, a small insertion in the CloDF13 ori sequence has been identified in one half of the plasmid dimer. The depositor has confirmed that this has not impacted plasmid function.
Proper citation: RRID:Addgene_201923 Copy
Vector Backbone Description: Vector Backbone:pBBR1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37294017
Proper citation: RRID:Addgene_188950 Copy
Vector Backbone Description: Vector Backbone:pBBR1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37294017
Proper citation: RRID:Addgene_188951 Copy
Vector Backbone Description: Vector Backbone:pBBR1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37294017
Proper citation: RRID:Addgene_188952 Copy
Species: Bacillus cytotoxicus
Genetic Insert: Integrase13 (tomato optimized)
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:pMC-6-ccdB-9; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36896468
Comments: Please visit https://doi.org/10.1101/2022.10.15.511792 for bioRxiv preprint.
Proper citation: RRID:Addgene_197728 Copy
Genetic Insert: HA-tagged CASP sigma
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36423276
Proper citation: RRID:Addgene_197614 Copy
Species: Synthetic
Genetic Insert: GvpJ-Linker-Large BiT
Vector Backbone Description: Vector Backbone:pET26b; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39227754
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.07.23.550228v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216975 Copy
Species: Synthetic
Genetic Insert: GvpK-Linker-Large BiT
Vector Backbone Description: Vector Backbone:pET26b; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39227754
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.07.23.550228v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216973 Copy
Species: Homo sapiens
Genetic Insert: FRB-Linker-Large BiT
Vector Backbone Description: Vector Backbone:pET26b; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39227754
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.07.23.550228v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216981 Copy
Species: Synthetic
Genetic Insert: For specific A-to-G base editing to introduce targeted A-to-G mutation in 16S rRNA in chloroplast
Vector Backbone Description: Vector Backbone:pK7WG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38128544
Comments: Ti vector constructed by multisite Gateway
Proper citation: RRID:Addgene_210834 Copy
Species: Synthetic
Genetic Insert: For specific A-to-G base editing to introduce targeted A-to-G mutation in nad7 in mitochondria
Vector Backbone Description: Vector Backbone:pK7WG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38128544
Comments: Ti vector constructed by multisite Gateway
Proper citation: RRID:Addgene_210832 Copy
Species: Synthetic
Genetic Insert: For specific A-to-G base editing to introduce targeted A-to-G mutation in ATP6-2 in mitochondria
Vector Backbone Description: Vector Backbone:pK7WG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38128544
Comments: Ti vector constructed by multisite Gateway
Proper citation: RRID:Addgene_210830 Copy
Species: Synthetic
Genetic Insert: For specific A-to-G base editing to introduce targeted A-to-G mutation in nad7 in mitochondria
Vector Backbone Description: Vector Backbone:pK7WG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38128544
Comments: Ti vector constructed by multisite Gateway
Proper citation: RRID:Addgene_210831 Copy
Genetic Insert: phage φX174 lysis gene E
Vector Backbone Description: Vector Backbone:CloDF13; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36714980
Proper citation: RRID:Addgene_224586 Copy
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