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Species: Synthetic
Genetic Insert: 29xGGGGCC 12xMS2 hairpins
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8700; Vector Backbone:pHR-TRE3G; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28562589
Comments: 29x GGGGCC cannot be confirmed by sequencing due to high GC content. Addgene was able to verify at least 16x repeats. Instability may be observed in the repeat region so it is recommended that multiple DNA preparations be screened.
Proper citation: RRID:Addgene_99149 Copy
Species: Synthetic
Genetic Insert: Ppsp [SD8] gIII(P29*,P83*,Y184*), luxAB; PproK pylT
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2343; Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29035361
Proper citation: RRID:Addgene_99212 Copy
Species: Synthetic
Genetic Insert: Ppsp [SD8] gIII(P29*,Y184*), luxAB; PproK pylT
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2343; Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29035361
Proper citation: RRID:Addgene_99211 Copy
Species: Synthetic
Genetic Insert: Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2921; Vector Backbone:SC101; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29035361
Proper citation: RRID:Addgene_99214 Copy
Species: Synthetic
Genetic Insert: Ppsp [SD4] T7RNAP(S12*,S203*)
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2729; Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29035361
Proper citation: RRID:Addgene_99218 Copy
Species: Synthetic
Genetic Insert: Ppsp [SD8] T7RNAP(S12*,S203*)
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2729; Vector Backbone:ColE1; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29035361
Proper citation: RRID:Addgene_99217 Copy
Species: Synthetic
Genetic Insert: Plpp chPyl(IPYE)RS; PproK pylT
Vector Backbone Description: Backbone Marker:Dieter Söll Lab; Backbone Size:1728; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29035361
Proper citation: RRID:Addgene_99222 Copy
Species: Synthetic
Genetic Insert: mScarlet
Vector Backbone Description: Backbone Marker:Hongkui Zeng/Allen Institute For Brain Science; Backbone Size:5719; Vector Backbone:AAV pCAG-FLEX-tdTomato-WPRE (Addgene #51503); Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: The stop codon for mRuby3 ends 24 bp downstream of the gene, adding an additional 8 amino acids to the protein. However, this is not expected to impact the function of the protein.
Proper citation: RRID:Addgene_99280 Copy
Species: Synthetic
Genetic Insert: TALE-VP64-T2A-TALE-VP64
Vector Backbone Description: Vector Backbone:pYPQ152; Vector Types:Plant Expression, TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29197638
Proper citation: RRID:Addgene_99325 Copy
Species: Synthetic
Genetic Insert: S. pyogenes sgRNA cassette
Vector Backbone Description: Backbone Size:9465; Vector Backbone:lentiGuide ( plasmid #52963); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28757163
Comments: Hygro sequence is from Addgene plasmid #61426 (generously shared by Feng Zhang Lab). "Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.", Konermann et al.. 2014
Proper citation: RRID:Addgene_99375 Copy
Species: Synthetic
Genetic Insert: S. pyogenes sgRNA cassette
Vector Backbone Description: Backbone Size:9465; Vector Backbone:lentiGuide ( plasmid #52963); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28757163
Comments: Hygro sequence is from Addgene plasmid #61426 (generously shared by Feng Zhang Lab). "Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.", Konermann et al.. 2014
mTagBFP2 sequence is from Addgene plasmid #34632 (generously shared by Vladislav Verkhusha Lab). "An enhanced monomeric blue fluorescent protein with the high chemical stability of the chromophore. ", Subach et al.. 2014
Proper citation: RRID:Addgene_99374 Copy
Species: Synthetic
Genetic Insert: S. pyogenes sgRNA cassette
Vector Backbone Description: Backbone Size:9465; Vector Backbone:lentiGuide ( plasmid #52963); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28757163
Comments: Hygro sequence is from Addgene plasmid #61426 (generously shared by Feng Zhang Lab). "Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.", Konermann et al.. 2014
iRFP670 sequence is codon-optimized for human (its original sequence is from Addgene plasmid #45453; generously shared by Vladislav Verkhusha Lab). "Near-infrared fluorescent proteins for multicolor in vivo imaging.", Shcherbakova et al.. 2013
Proper citation: RRID:Addgene_99377 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/298620 for bioRxiv preprint.
Proper citation: RRID:Addgene_99672 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pAAV (pUC f1); Vector Types:AAV; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/298620 for bioRxiv preprint.
Proper citation: RRID:Addgene_99679 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pAAV (pUC f1); Vector Types:AAV; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/298620 for bioRxiv preprint.
Proper citation: RRID:Addgene_99680 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pAAV (pUC f1); Vector Types:AAV; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/298620 for bioRxiv preprint.
Proper citation: RRID:Addgene_99685 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pAAV (pUC f1); Vector Types:AAV; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/298620 for bioRxiv preprint.
Proper citation: RRID:Addgene_99687 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pAAV (pUC f1); Vector Types:AAV; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/298620 for bioRxiv preprint.
Proper citation: RRID:Addgene_99686 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pAAV (pUC f1); Vector Types:AAV; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/298620 for bioRxiv preprint.
Proper citation: RRID:Addgene_99689 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pAAV (pUC f1); Vector Types:AAV; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/298620 for bioRxiv preprint.
Proper citation: RRID:Addgene_99688 Copy
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