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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pETM33_Nsp3e_NAB Resource Report Resource Website |
RRID:Addgene_156473 | Nsp3e_NAB | Synthetic | Kanamycin | PMID:37704626 | insert amino acid sequence: GKKDNSYFTEQPIDLVPNQPYPNASFDNFKFVCDNIKFADDLNQLTGYKKPASRELKVTFFPDLNGDVVAIDYKHYTPSFKKGAKLLHKPIVWHVNNATNKATYKPNTWCIRCLWSTKPVETx | Backbone Size:6017; Vector Backbone:pETM33; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Gene insert is codon optimized for Ecoli. | 2026-08-15 01:08:05 | 0 |
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pETM33_Nsp15 Resource Report Resource Website 1+ mentions |
RRID:Addgene_156481 | Nsp15 | Synthetic | Kanamycin | PMID:37704626 | insert amino acid sequence: GLQSLENVAFNVVNKGHFDGQQGEVPVSIINNTVYTKVDGVDVELFENKTTLPVNVAFELWAKRNIKPVPEVKILNNLGVDIAANTVIWDYKRDAPAHISTIGVCSMTDIAKKPTETICAPLTVFFDGRVDGQVDLFRNARNGVLITEGSVKGLQPSVGPKQASLNGVTLIGEAVKTQFNYYKKVDGVVQQLPETYFTQSRNLQEFKPRSQMEIDFLELAMDEFIERYKLEGYAFEHIVYGDFSHSQLGGLHLLIGLAKRFKESPFELEDFIPMDSTVKNYFITDAQTGSSKCVCSVIDLLLDDFVEIIKSQDLSVVSKVVKVTIDYTEISFMLWCKDGHVETFYPKLx | Backbone Size:6017; Vector Backbone:pETM33; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Gene insert is codon optimized for Ecoli. | 2026-08-15 01:08:05 | 1 |
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pHis-LMW_FGF2 Resource Report Resource Website |
RRID:Addgene_157657 | Fibroblast growth factor 2 | Homo sapiens | Kanamycin | PMID:32383752 | Backbone Size:5369; Vector Backbone:pHis; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | changed Cystein 211,229 to serine, deleted amino acids 1-134 | 2026-08-15 01:08:07 | 0 | |
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pET28b-API5 Resource Report Resource Website |
RRID:Addgene_157655 | Apoptosis inhibitor 5 | Homo sapiens | Kanamycin | PMID:32383752 | Backbone Size:5369; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:08:06 | 0 | ||
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pET28a-Coh-ddFLN4-ELP(MV7E2)3-His-ybbR Resource Report Resource Website |
RRID:Addgene_157672 | Cohesin (R.f) with ddFLN4, ELP, Histag and ybbR tag | Other | Kanamycin | PMID:33191756 | Backbone Size:5200; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:08:07 | 0 | ||
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pDH001 Resource Report Resource Website |
RRID:Addgene_157639 | MHT | Batis maritima | Kanamycin | PMID:33104325 | Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:08:06 | 0 | ||
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pDH007 Resource Report Resource Website |
RRID:Addgene_157642 | sMHT(1-113) -CheA | Kanamycin | PMID:33104325 | Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:08:06 | 0 | |||
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pDH006 Resource Report Resource Website |
RRID:Addgene_157641 | sMHT(1-113) | Kanamycin | PMID:33104325 | Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:08:07 | 0 | |||
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pDH003 Resource Report Resource Website |
RRID:Addgene_157640 | sMHT(1-113)-SYNZIP17 | Kanamycin | PMID:33104325 | Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:08:06 | 0 | |||
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pCMV Aquamarine Resource Report Resource Website |
RRID:Addgene_157777 | Kanamycin | PMID:31317736 | Backbone Marker:Clontech; Backbone Size:3939; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:08:08 | 0 | ||||
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pelBHisTEV Resource Report Resource Website |
RRID:Addgene_157741 | Kanamycin | - Empty vector (kanamycin-R) adds TEV-protease-removable N-terminal PelB signal sequence + His10, plus potential C-term His6. Non-leaky expression due to T7lac promoter. - Has an advantage in leaving no added amino acid residues following TEV protease cleavage of PelB signal sequence + His10. This advantage is made possible by cloning into the two BseRI restriction sites. - Subcloning requires ligation-independent cloning and use of the two BseRI restriction sites that are present in this empty vector. The two BseRI sites span a removable 432 nucleotide sequence. Cloning into the BseRI-digested vector can be achieved using a ligation-independent system that is dependent on homologous recombination, such as the Takara In-Fusion HD Cloning Plus system, which requires simply designing the PCR-derived insert (containing the protein of interest) to contain 15 bp at each end that matches the 15 bp at each end of the linearized parent vector. In other words, add these 15 bp, AACCTGTACTTCCAA, to the 5' end of the PCR FORWARD primer, and add these 15 bp, GTGGTGGTGCTCGAG, to the 5' end of the PCR REVERSE primer. - The sequence of the added N-terminus is the PelB signal sequence (M1-A22 of GenBank #AAA24848) + 13 aa linker + His10-tag + 4 aa linker + TEV protease cleavage site. The N-terminal protein sequence is: MKYLLPTAAAGLLLLAAQPAMA + MDIGINSDPNSSS + HHHHHHHHHH + PMGS + ENLYFQ*X, where * is the TEV protease cleavage site and X is the N-terminal residue of the introduced protein of interest. Note that TEV protease cleaves most efficiently when X = S, G, A, M; for other compatible residues see Kapust et al 2002, PMID 12074568. - A C-terminal His6-tag is possible: if no stop codon is included in the cloned insert, the sequence LEHHHHHH is added to the C-terminus. - The PelB signal sequence directs the protein of interest to the E. coli inner membrane. Whether the protein traverses the inner membrane is dependent on the protein of interest. - BseRI cuts 8-10 nucleotides outside of its own recognition sequence and is a non-palindromic site. The placement & orientation of the two BseRI sites eliminates all of the BseRI recognition sequence in the resulting subclone. - To verify the PCR-generated insert sequence, use sequencing primers T7 (TAATACGACTCACTATAGGG), T7-Ter (GCTAGTTATTGCTCAGCGG). | Backbone Size:5401; Vector Backbone:pelB-MBP (DNASU access. no. EvNO00813783); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:08:08 | 0 | ||||
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pEGFP N1 AURKA 1-110 Resource Report Resource Website |
RRID:Addgene_157755 | AURKA | Homo sapiens | Kanamycin | PMID:30070631 | Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | The first 110 amino acids of AURKA were cloned | 2026-08-15 01:08:08 | 0 | |
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SARS-CoV-2_N_deltaNTD Resource Report Resource Website |
RRID:Addgene_157869 | N | SARS-CoV-2 | Kanamycin | PMID:33200826 | Vector Backbone:pTHMT; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | deltaNTD | 2026-08-15 01:08:09 | 0 | |
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hnRNPA2_RRM2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_157866 | hnRNPA2 | Homo sapiens | Kanamycin | PMID:32870271 | Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:08:09 | 1 | ||
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hnRNPA2_RRM1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_157865 | hnRNPA2 | Homo sapiens | Kanamycin | PMID:32870271 | Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:08:09 | 1 | ||
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SARS-CoV-2_N_deltaCIDR Resource Report Resource Website |
RRID:Addgene_157872 | N | SARS-CoV-2 | Kanamycin | PMID:33200826 | Please note: This plasmid contains a D15H mutation in MBP. This mutation is not known to affect plasmid function. | Vector Backbone:pTHMT; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | deltaCIDR | 2026-08-15 01:08:10 | 0 |
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SARS-CoV-2_N_deltaCTD Resource Report Resource Website |
RRID:Addgene_157871 | N | SARS-CoV-2 | Kanamycin | PMID:33200826 | Vector Backbone:pTHMT; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | deltaCTD | 2026-08-15 01:08:09 | 0 | |
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Expression_vector_RO2_10 Resource Report Resource Website |
RRID:Addgene_158019 | de novo designed protein RO2_10 | Synthetic | Kanamycin | PMID:32855341 | Please visit https://www.biorxiv.org/content/10.1101/2020.04.14.041772v1 for bioRxiv preprint. | Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:08:11 | 0 | |
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Expression_vector_RO2_9 Resource Report Resource Website |
RRID:Addgene_158018 | de novo designed protein RO2_9 | Synthetic | Kanamycin | PMID:32855341 | Please visit https://www.biorxiv.org/content/10.1101/2020.04.14.041772v1 for bioRxiv preprint. | Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:08:11 | 0 | |
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Expression_vector_RO1_8 Resource Report Resource Website |
RRID:Addgene_158013 | de novo designed protein RO1_8 | Synthetic | Kanamycin | PMID:32855341 | Please visit https://www.biorxiv.org/content/10.1101/2020.04.14.041772v1 for bioRxiv preprint. | Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:08:11 | 0 |
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