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Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: ORF6
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_141275 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: ORF7A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5585; Vector Backbone:pDONR207; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_141276 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: ORF3B
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_141272 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:10844; Vector Backbone:pGWB401; Vector Types:Plant Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:31316580
Comments: Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium).
Proper citation: RRID:Addgene_141285 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11165; Vector Backbone:pGWB501; Vector Types:Plant Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:31316580
Comments: Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium).
Proper citation: RRID:Addgene_141286 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:10700; Vector Backbone:pGWB601; Vector Types:Plant Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:31316580
Comments: Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium).
Proper citation: RRID:Addgene_141287 Copy
Species: Ruminococcus flavefaciens XPD3002
Genetic Insert: Rfx-Cas13d
Vector Backbone Description: Backbone Size:3150; Vector Backbone:pT3TS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32768421
Comments: This plasmid is owned by Moreno-Mateos Lab (CABD, Seville, Spain) and Bazzini Lab (Stowers Institute, Kansas, MO, USA)
Proper citation: RRID:Addgene_141320 Copy
Species: Homo sapiens
Genetic Insert: TARDBP-WT
Vector Backbone Description: Backbone Marker:Addgene# 24588; Backbone Size:9389; Vector Backbone:pLD-puro-Cc-VA (MAPLE vector); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32325033
Proper citation: RRID:Addgene_141327 Copy
Species: Synthetic
Genetic Insert: MBP-NLUC3F10H
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5369; Vector Backbone:pET-28 a (+); Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:31316580
Proper citation: RRID:Addgene_141291 Copy
Species: Homo sapiens
Genetic Insert: GB1-His6-TEV-ALIX(703-800)-Strep
Vector Backbone Description: Backbone Size:5643; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32917811
Proper citation: RRID:Addgene_141344 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS305; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: integrating.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14137 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS305; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: integrating.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14138 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS304; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: integrating.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14136 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS303; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: integrating.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14133 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Addgene #25896; Backbone Size:9573; Vector Backbone:pLX302; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_141348 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS306; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: integrating.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14188 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS305; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: integrating.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14186 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS305; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: integrating.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14185 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS304; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: integrating.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14183 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS414; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: CEN.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14192 Copy
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