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Species: Synthetic
Genetic Insert: LB_(etr)8-(C120)5-PhCMVmin-GBP-mCherry-T35S_RB
Vector Backbone Description: Vector Backbone:Xpre2-S; Vector Types:Synthetic Biology, Eukaryotic Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32601426
Proper citation: RRID:Addgene_155324 Copy
Species: S. pyogenes
Genetic Insert: dCas9 from S. pyogenes
Vector Backbone Description: Backbone Marker:Takara; Vector Backbone:pMD19T simple; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26689101
Proper citation: RRID:Addgene_73223 Copy
Species: S. pyogenes
Genetic Insert: dCas9 from S. pyogenes
Vector Backbone Description: Backbone Marker:Takara; Vector Backbone:pMD19T simple; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26689101
Proper citation: RRID:Addgene_73220 Copy
Species: Synthetic
Genetic Insert: BFPKRAB_PUFa
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates and protocols
Proper citation: RRID:Addgene_73692 Copy
Species: Synthetic
Genetic Insert: ratiometric pHluorin2
Vector Backbone Description: Vector Backbone:PCR8; Vector Types:middle entry clone; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24973752
Comments: Ratiometric pHluorin2 was originally published in the following paper:
Mahon, M. (2011) pHluorin2: an enhanced, ratiometric, pH-sensitive green florescent protein. Advances in Bioscience and Biotechnology, 2, 132-137. doi: 10.4236/abb.2011.23021.
Proper citation: RRID:Addgene_73794 Copy
Species: bacteria
Genetic Insert: bar and GUS cassettes
Vector Backbone Description: Backbone Size:7132; Vector Backbone:pPZP201; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:18256901
Comments: Discrepancies between Depositor's full sequence and Addgene's QC sequence are not of functional concern.
Proper citation: RRID:Addgene_73934 Copy
Species: Synthetic
Genetic Insert: bar
Vector Backbone Description: Backbone Size:7132; Vector Backbone:pPZP201; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:15034747
Comments: This vector is suitable for plant transformation and has been used as standard service empty vector control.
Proper citation: RRID:Addgene_73933 Copy
Species: Synthetic
Genetic Insert: bar
Vector Backbone Description: Backbone Size:7132; Vector Backbone:pPZP201; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:15034747
Comments: This vector is suitable hpRNA-mediated gene silencing in tissue (seed) specific manner.
Please see the Plant Transformation Core Facility website for more information on using this plasmid: http://plantsci.missouri.edu/muptcf/pzy101.html
The vector has following features:
1. It has two replicate origins that allow to replicate in both E. coli and Agrobacterium tumefaciens cells (at higher copy numbers).
2. It also has bom site that allows you to use tri-parental mating to mobilize the vector to Agrobacterium cells.
3. The aadA locus confers Spectinomycin (100 mg/L) or streptomycin (100 mg/L) resistance and thus enable to use either or both of these two antibiotics for the vector selection.
4. The multiple cloning site (MCS) adjacent to the double CaMV35S promoter will allow you to insert your cassette. This CaMV35S promoter is used to drive the bar ORF and, therefore, is not used in front to drive your gene of interest. You need to assemble your own expression cassette including promoter, gene coding sequence, and terminator, and then insert to anywhere between Hind III and EcoR I within the MCS.
Special notes:
1. Insert your gene expression cassette in such a way that your promoter is adjacent to the promoter driving the bar gene selectable marker. This is to prevent transcription read-through from your gene expression cassette.
2. In order to use the EcoR I site within the MCS, a minimal ratio of this enzyme versus DNA is recommended to avoid star activity. When HindIII and EcoRI are in combined use, even less of each enzyme is required, and reaction should be conducted in a large volume to avoid excessive enzymatic activity.
Proper citation: RRID:Addgene_73932 Copy
Species: Synthetic
Genetic Insert: sgRNA pyk (NT)
Vector Backbone Description: Vector Backbone:pAL374; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26829286
Proper citation: RRID:Addgene_74072 Copy
Species: Synthetic
Genetic Insert: sgRNA pyk (T)
Vector Backbone Description: Vector Backbone:pAL374; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26829286
Proper citation: RRID:Addgene_74070 Copy
Species: Synthetic
Genetic Insert: sgRNA rfp (T)
Vector Backbone Description: Vector Backbone:pAL374; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26829286
Proper citation: RRID:Addgene_74075 Copy
Genetic Insert: miniSOG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2818; Vector Backbone:pCR8; Vector Types:Gateway TOPO Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26861262
Proper citation: RRID:Addgene_74111 Copy
Species: Homo sapiens
Genetic Insert: Magneto2.0
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2817; Vector Backbone:pCR8; Vector Types:Gateway vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26950006
Proper citation: RRID:Addgene_74334 Copy
Species: Homo sapiens
Genetic Insert: Magneto2.0
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8; Vector Types:Gateway vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26950006
Proper citation: RRID:Addgene_74333 Copy
Species: Synthetic
Genetic Insert: proUBQ10::PIP2A-mCherry-NOSt_proUBQ10::GFP-ADHt
Vector Backbone Description: Vector Backbone:JAB2120; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35951698
Comments: Backbone JAB2120 contains proUBQ10::PIP2A-mCherry-NOSt. Please see the fully annotated sequence linked the "Supplemental Documents" section. Please visit https://www.biorxiv.org/content/10.1101/2022.02.02.478917v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_190413 Copy
Species: Epstein-Barr virus
Genetic Insert: BXRF1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2875; Vector Backbone:pENTR223.1; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17446270
Proper citation: RRID:Addgene_195789 Copy
Species: Synthetic
Genetic Insert: proUBQ10::PIP2A-mCherry-NOSt_pro35S-BetIO::GFPint-NLS_ADHt
Vector Backbone Description: Vector Backbone:JAB2120; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35951698
Comments: Backbone JAB2120 contains proUBQ10::PIP2A-mCherry-NOSt. Please see the fully annotated sequence linked the "Supplemental Documents" section. Please visit https://www.biorxiv.org/content/10.1101/2022.02.02.478917v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_190379 Copy
Species: Epstein-Barr virus
Genetic Insert: BARF1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2875; Vector Backbone:pENTR223.1; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17446270
Proper citation: RRID:Addgene_195783 Copy
Species: Epstein-Barr virus
Genetic Insert: BSRF1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2875; Vector Backbone:pENTR223.1; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17446270
Proper citation: RRID:Addgene_195782 Copy
Species: Epstein-Barr virus
Genetic Insert: BDLF4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2875; Vector Backbone:pENTR223.1; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17446270
Proper citation: RRID:Addgene_195785 Copy
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