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Species: Homo sapiens
Genetic Insert: Protein Kinase C iota
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:11076; Vector Backbone:pWPI; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please note that the vector backbone, pWPI, is a bicistronic vector that allows for simultaneous expression of a transgene (PRKCI in this case) and an EGFP marker to facilitate tracking of transduced cells. The EGFP marker cDNA has been inserted downstream of EMCV IRES.
The PRKCI insert in this plasmid is a known variant that lack the first nine amino acids of PRKCI when compared to GenBank entry NP_002731.4
Proper citation: RRID:Addgene_35387 Copy
Species: Homo sapiens
Genetic Insert: hPLK2
Vector Backbone Description: Backbone Marker:Didier Trono; Backbone Size:11437; Vector Backbone:pWPI/Neomycin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21849493
Comments: The backbone of this bicistronic lentiviral vector was originally created by Dr. Didier Trono lab (pWPI, Plasmid # 12254), and then modified by Robert Strome using Neomycin to replace EGFP and adding several multiple clone sites. The MCS includes Swa I, Nde I, BamH I, Spe I, Xma I and Sma I (see sequence). The hPlk2WT cDNA was inserted with BamH I site, and can be replaced by any other gene with this site. This vector allows the simultaneous expression of hPlk2WT and Neomycin to facilitate establishing stable mammalian transgene cell lines.
Please note that the full sequence for this plasmid is approximated and not fully verified. For further cloning strategies, please contact with Dr. Anurag Tandon.
Proper citation: RRID:Addgene_35385 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:4191; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000118; Origin: BBR1
Proper citation: RRID:Addgene_35352 Copy
Species: Homo sapiens
Genetic Insert: eNpHR 3.0
Vector Backbone Description: Backbone Size:5609; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20303157
Comments: This plasmid contains the human elongation factor-1a promoter. Golgi export trafficking signal (TS) sequence is KSRITSEGEYIPLDQIDINV. ER export sequence is FCYENEV.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26966 Copy
Species: Homo sapiens
Genetic Insert: hChR2(E123T/H134R)-EYFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5587; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20081849
Comments: This plasmid contains the human elongation factor-1a promoter.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26968 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sus1
Vector Backbone Description: Backbone Size:3669; Vector Backbone:PRSF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20395473
Proper citation: RRID:Addgene_26961 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: sgf11
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:20395473
Comments: sgf 73(1-96) cloned in the second frame by NdeI+BglII for coexpression
Proper citation: RRID:Addgene_26964 Copy
Species: Homo sapiens
Genetic Insert: shRNA NEDD4L #1
Vector Backbone Description: Backbone Size:6401; Vector Backbone:pRetroSuper; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19917253
Comments: shRNA sequence - 5'-gatccccGCTAGACTGTGGATTGAGTttcaagagaACTCAATCCACAGTCTAGCtttttggaaa
Proper citation: RRID:Addgene_27016 Copy
Species: Homo sapiens
Genetic Insert: hChR2(H134R)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4568; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: This plasmid contains the human synapsin I promoter.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26976 Copy
Species: Homo sapiens
Genetic Insert: hChR2(H134R)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4574; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: This plasmid contains the human synapsin I promoter.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26973 Copy
Species: Homo sapiens
Genetic Insert: hChR2(H134R)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5386; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: This plasmid contains the mouse calcium/calmodulin-dependent kinase II alpha subunit promoter.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26975 Copy
Species: Homo sapiens
Genetic Insert: LEF1
Vector Backbone Description: Backbone Marker:Addgene Plasmid 1764; Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19576624
Proper citation: RRID:Addgene_27023 Copy
Species: Caenorhabditis elegans
Genetic Insert: histone H2B
Vector Backbone Description: Backbone Size:0; Vector Backbone:pie-1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18692039
Comments: 1st generation pie-1 vector
Note that there are some discrepancies between the Addgene QC sequence and the depositor's sequence. They should not affect function.
Proper citation: RRID:Addgene_26946 Copy
Species: synthetic gene
Genetic Insert: P#38-mcherry rfp
Vector Backbone Description: Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Proper citation: RRID:Addgene_27058 Copy
Species: Homo sapiens
Genetic Insert: EYFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5603; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: This plasmid contains the human elongation factor-1a promoter.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_27056 Copy
Species: Homo sapiens
Genetic Insert: hChR2(H134R)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6290; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: This plasmid contains the human GFAP promoter.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_27055 Copy
Species: Mus musculus
Genetic Insert: CRel
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pMIG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16166378
Proper citation: RRID:Addgene_26984 Copy
Species: Gallus gallus
Genetic Insert: c-Src
Vector Backbone Description: Backbone Marker:Addgene Plasmid 1765; Backbone Size:5558; Vector Backbone:pBABE-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19573813
Comments: GenBank: V00402.1
Proper citation: RRID:Addgene_26983 Copy
Species: Homo sapiens
Genetic Insert: shRNA Fyn
Vector Backbone Description: Backbone Size:6401; Vector Backbone:pRetroSuper; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19573813
Comments: shRNA sequence - 5'-GAACTTCCATGGCCCTCAT
Proper citation: RRID:Addgene_26985 Copy
Species: Homo sapiens
Genetic Insert: shRNA Src
Vector Backbone Description: Backbone Size:6401; Vector Backbone:pRetroSuper; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19573813
Comments: shRNA sequence - 5'-GGACCTTCCTCGTGCGAGA
Proper citation: RRID:Addgene_26982 Copy
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