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Species: Mus musculus
Genetic Insert: R2pH-LAMP1-3xFLAG
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32515674
Proper citation: RRID:Addgene_157940 Copy
Species: Mus musculus
Genetic Insert: mH2A.Z-EGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pIND; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_15770 Copy
Species: Mus musculus
Genetic Insert: MannII
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33097540
Comments: Existing fluorescent protein in plasmid Adggene# 56332 was replaced with mGold.
"N-10" of the name of the plasmid indicates the position of the tag (N- or C-terminal with respect to the fluorescent protein) and the number of residues in the linker between the two proteins. Please note that the mVenus annotation in the plasmid map is incorrect, as this plasmid encodes mGold.
Proper citation: RRID:Addgene_158005 Copy
Species: Mus musculus
Genetic Insert: CAF1
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33097540
Comments: Existing fluorescent protein in plasmid Adggene# 55953 was replaced with mGold.
"C-10" of the name of the plasmid indicates the position of the tag (N- or C-terminal with respect to the fluorescent protein) and the number of residues in the linker between the two proteins. Please note that the mVenus annotation in the plasmid map is incorrect, as this plasmid encodes mGold.
Proper citation: RRID:Addgene_158003 Copy
Species: Mus musculus
Genetic Insert: hemicholinium-3-sensitive choline transporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11709061
Proper citation: RRID:Addgene_15765 Copy
Species: Mus musculus
Genetic Insert: POSH
Vector Backbone Description: Backbone Size:4750; Vector Backbone:pRK5-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9482736
Proper citation: RRID:Addgene_15908 Copy
Species: Mus musculus
Genetic Insert: ptch1
Vector Backbone Description: Vector Backbone:pVLAD6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30415841
Proper citation: RRID:Addgene_120886 Copy
Species: Mus musculus
Genetic Insert: Foxo1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16154098
Comments: Note that a BLAST search with Addgene's quality control sequence shows a K219R mutation. The Accili lab has explained that this is a common variant and they do not consider it a mutation at all. They have used the plasmid routinely without issue. The plasmid also contains an L619P polymorphism.
Proper citation: RRID:Addgene_12148 Copy
Species: Mus musculus
Genetic Insert: Foxo1
Vector Backbone Description: Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11696581
Comments: Plasmid contains K219R and L619P polymorphisms, which are not known to affect function.
Proper citation: RRID:Addgene_12142 Copy
Species: Mus musculus
Genetic Insert: ptch1
Vector Backbone Description: Vector Backbone:pcDNA-h; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30415841
Proper citation: RRID:Addgene_120903 Copy
Species: Mus musculus
Genetic Insert: p53
Vector Backbone Description: Backbone Size:5010; Vector Backbone:pLCR0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1761576
Comments: This plasmid, containing
the wild type murine p53 cDNA under the control of the previously
described BPV LCR-SV40 hybrid promoter (Spaholz et al., 1985),
was constructed as follows. Plasmid p407-1 (Spaholz et al., 1985) was
digested with EcoRI and HindIII, and the 5010-base pair fragment
containing the LCR-SV40 hybrid promoter was isolated and filled in
with Klenow.
After the ligation of BamHI linkers to the ends of the fragment, regenerating the terminal EcoRI site, the fragment was digested with
BamHI and ligated to the 2334-base pair BamHI fragment from the p53 cDNA expression vector pll-4 (Tan et al., 1986). This BamHI
fragment contained the entire wild type murine p53 coding region, 50 base pairs of 5'-untranslated sequence, 287 base pairs of 3'-untranslated
sequence, and a 3'-located SV40 mRNA splice site and polyA signal.
Proper citation: RRID:Addgene_12136 Copy
Species: Mus musculus
Genetic Insert: GBS(long)-GFP-insulator
Vector Backbone Description: Vector Backbone:pJC4-5; Vector Types:mouse transgenesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22265416
Proper citation: RRID:Addgene_121975 Copy
Species: Mus musculus
Genetic Insert: Myocardin
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pShuttle; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19342595
Comments: Myocardin contains an A435T difference compared to the Myocd NCBI entry. The functional consequence of this difference is unknown.
Proper citation: RRID:Addgene_122122 Copy
Species: Mus musculus
Genetic Insert: MEKK3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14634666
Proper citation: RRID:Addgene_12189 Copy
Species: Mus musculus
Genetic Insert: MEKK1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1/HisB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9528810
Comments: Please note that Addgene's sequencing results identified a V1467L mutation in the MEKK1 sequence when compared to GenBank ID AF117340.1. The depositing laboratory states that this plasmid functions exactly as intended and the mutation may represent a valid polymorphism.
Proper citation: RRID:Addgene_12181 Copy
Species: Mus musculus
Genetic Insert: MEKK2
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8621389
Proper citation: RRID:Addgene_12182 Copy
Species: Mus musculus
Genetic Insert: Hmga1a
Vector Backbone Description: Backbone Marker:Yukiko Gotoh, Addgene plasmid #122292; Vector Backbone:pCAG2IG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22797695
Comments: Please note: Addgene NGS unable to fully resolve CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
Proper citation: RRID:Addgene_122293 Copy
Species: Mus musculus
Genetic Insert: Mus musculus proprotein convertase subtilisin/kexin type 2 (Pcsk2)
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30104250
Proper citation: RRID:Addgene_122673 Copy
Species: Mus musculus
Genetic Insert: TLR2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFlag-CMV-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832055
Comments: Murine TLR2 expression vector was made by removing the original signal peptide and replacing it with the signal peptide from preprotrypsin. When compared to GenBank reference NP_036035.3, the TLR2 insert in this plasmid appears to be missing aa1-23 due to the removal of the endogenous TLR2 signal peptide. See associated publication for more information.
Also, please note that Addgene's sequencing results identified additional mutations M82I and H202Y when compared to GenBank reference NP_036035.3. It is not known whether these mutations affect TLR2 function.
Proper citation: RRID:Addgene_12291 Copy
Species: Mus musculus
Genetic Insert: TLR2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFlag-CMV-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832055
Comments: Murine TLR2 expression vector was made by removing the original signal peptide and replacing it with the signal peptide from preprotrypsin. When compared to GenBank reference NP_036035.3, the TLR2 insert in this plasmid appears to be missing aa1-23 due to the removal of the endogenous TLR2 signal peptide. See associated publication for more information.
Also, please note that Addgene's sequencing results identified additional mutations A74V, M82I and H202Y when compared to GenBank reference NP_036035.3. M82I and H202Y are possible polymorphisms as they were also present in the wild-type TLR2 sequence from the depositing laboratory (Addgene Plasmid 12291: pCMV-M2-TLR2). It is not known whether these mutations affect TLR2 function.
Proper citation: RRID:Addgene_12290 Copy
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