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Species: canine
Genetic Insert: dog ZO-2 shRNA
Vector Backbone Description: Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19605556
Proper citation: RRID:Addgene_37211 Copy
Genetic Insert: coxM1′::lacZ reporter system of pUX3009 with an improved −35/ −10 RNAP binding region AND RcoM1 with C-term 6xHIS tag
Vector Backbone Description: Vector Backbone:pUX3272 AND pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3272 (pUX3009 based coxM1′::lacZ reporter system with binding sites “d + e + f” and an improved −35/−10 RNAP binding region (5′-TTGACA-(18N)-TATCCT-3′) ) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
Strain is CmR, pUX3272 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan.
For strain usage see attached pdf.
Proper citation: RRID:Addgene_37179 Copy
Genetic Insert: ~5-kb BamHI + XhoI fragment from pUX2996 cloned into BamHI + SalI-cut pEXT20
Vector Backbone Description: Vector Backbone:pUX3025; Vector Types:; Bacterial Resistance:Ampicillin
Comments: This strain contains Roberts lab plasmid pUX3025 which contains ~5-kb BamHI + XhoI fragment from pUX2996 cloned into BamHI + SalI-cut pEXT20. Useful for mutagenesis and excision of the “e + f”
binding region.
For strain usage details see attached pdf. Please note that this strain has not been tested functionally by Addgene.
Proper citation: RRID:Addgene_37173 Copy
Genetic Insert: ~5-kb BamHI + XhoI fragment from pUX3010 cloned into BamHI + SalI-cut pEXT20
Vector Backbone Description: Vector Backbone:pUX3057; Vector Types:; Bacterial Resistance:Ampicillin
Comments: This strain contains Roberts lab plasmid pUX3057 which contains ~5-kb BamHI + XhoI fragment from pUX3010 cloned into BamHI + SalI-cut pEXT20. Useful for mutagenesis and excision of the “a-f”
binding region.
For strain usage details see attached pdf. Please note that Addgene has not tested this strain functionally.
Proper citation: RRID:Addgene_37175 Copy
Genetic Insert: coxM1′::lacZ reporter system of pUX3009 with “e” binding site “TTnnnG” motif changed to “GGnnnG” AND RcoM1 with C-term 6xHIS tag
Vector Backbone Description: Vector Backbone:pUX3297 AND pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3297 (pUX3009 based coxM1′::lacZ reporter system with binding sites “d + e + f” with the “e” binding site “TTnnnG” motif changed to “GGnnnG;”) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
Strain is CmR, pUX3297 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan.
For strain usage see attached pdf.
Proper citation: RRID:Addgene_37180 Copy
Species: canine
Genetic Insert: dog ZO-1 shRNA
Vector Backbone Description: Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19605556
Proper citation: RRID:Addgene_37206 Copy
Genetic Insert: coxM1′::lacZ reporter system with RcoM binding site "f" AND RcoM1 with C-term 6xHIS tag
Vector Backbone Description: Vector Backbone:pUX3008 AND pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3008 (coxM1′::lacZ reporter system with binding site “f”) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
Strain is CmR, pUX3008 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan.
For strain usage see attached pdf.
Proper citation: RRID:Addgene_37169 Copy
Genetic Insert: coxM1′::lacZ reporter system AND Burkholderia xenovorans RcoM1 with C-term 6xHIS tag
Vector Backbone Description: Vector Backbone:pUX2996 and pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX2996 (pUX2970 with a coxM1′::lacZ reporter system such that ...vector DNA – BamHI (unique site) – “e + f” binding region – coxM1′(with unique SalI site)::lacZ-> tfd <-KanR (with 5′-end unique XhoI site) – vector DNA <-SpR...) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
Strain is CmR, plasmid pUX2996 is KanR and SpecR and plasmid is pUX2410 is AmpR. Can be grown in media containing Amp + Kan.
For strain usage see attached pdf. Please note that this strain has not been tested functionally by Addgene.
Proper citation: RRID:Addgene_37167 Copy
Species: Mus musculus
Genetic Insert: Rosa26 left targeting arm
Vector Backbone Description: Backbone Marker:Sigma; Vector Backbone:pZDonor AAVS1; Vector Types:Genomic targeting - zinc finger; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22169954
Comments: The homology arms in the ROSA26 donor vector were generated by PCR of mouse genomic DNA and insertion of the PCR-amplified right homology arm (left primer: 5-TTATTGCGGCCGCAG ATGGGCGGGAGTCTTCT-3, right primer: 5-AACA CCGCGGCAGTTTATAAATGGAGAAAAAGGAGA -3) between the NotI and SacII sites and the left homology arm (left primer: 5-TCTATGGTACCAGTTAACGGCA GCCGGAGT-3 , right primer: 5 -CGGACGAATTCTCT AGAAAGACTGGAGTTGCAGA-3) between the KpnI and EcoRI sites in the pZDonor AAVS1 vector obtained from Sigma–Aldrich.
Proper citation: RRID:Addgene_37200 Copy
Species: Burkholderia xenovorans
Genetic Insert: rcoM2 in pEXT20
Vector Backbone Description: Vector Backbone:pEXT20; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18326575
Comments: This strain contains Roberts lab plasmid pUX2397 which contains an EcoRI-HindIII fragment bearing Burkholderia xenovorans LB400 rcoM2 cloned into pEXT20. Suitable for expression of Burkholderia xenovorans RcoM2 protein (untagged). Host strain is E. coli VJS6737.
For strain usage see attached pdf.
Proper citation: RRID:Addgene_37161 Copy
Species: Burkholderia xenovorans
Genetic Insert: rcoM1 in pEXT20
Vector Backbone Description: Vector Backbone:pUX2410; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18326575
Comments: This strain contains Roberts lab plasmid pUX2410 which is an pUX2396 derivative suitable for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag. Host strain is E. coli VJS6737.
For strain usage see attached pdf.
Proper citation: RRID:Addgene_37162 Copy
Species: Burkholderia xenovorans
Genetic Insert: ~1200-bp fragment from Burkholderia xenovorans LB400 cloned into pEXT20
Vector Backbone Description: Vector Backbone:pUX2442; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:18326575
Comments: This strain contains Roberts lab plasmid pUX2442 which contains ~1200bp fragment from Burkholderia xenovorans LB400 with introduced terminal HindIII and SalI sites [such that HindIII – (<-rcoM1
– intergenic region – coxM1′->) – SalI] cloned into pEXT20 with the lacZ + KanR cassette from pKOK6 cloned at the SalI site such that coxM1′::lacZ->tfd<-KanR. Suitable template for amplification of the Burkholderia xenovorans RcoM1 binding region + reporter cassette.
For strain usage see attached pdf.
Proper citation: RRID:Addgene_37164 Copy
Genetic Insert: coxM1′::lacZ reporter system with RcoM binding sites "d + e + f"
Vector Backbone Description: Vector Backbone:pUX3009; Vector Types:; Bacterial Resistance:Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmid pUX3009 (pUX2996 based coxM1′::lacZ reporter system with RcoM binding sites "d + e + f").
Strain is CmR, pUX3007 is KanR and SpecR. Can be grown on Kan only.
For strain usage see attached pdf.
Proper citation: RRID:Addgene_37170 Copy
Genetic Insert: coxM1′::lacZ reporter system with RcoM binding sites "d + e + f" AND RcoM1 with C-term 6xHIS tag
Vector Backbone Description: Vector Backbone:pUX3009 AND pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3009 (coxM1′::lacZ reporter system with binding sites “d + e + f”) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
Strain is CmR, pUX3009 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan.
For strain usage see attached pdf.
Proper citation: RRID:Addgene_37171 Copy
Species: Homo sapiens
Genetic Insert: lig4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4776; Vector Backbone:pFASTBAC1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_37150 Copy
Species: Homo sapiens
Genetic Insert: XRCC6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4776; Vector Backbone:pFASTBAC1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_37148 Copy
Species: Homo sapiens
Genetic Insert: XRCC5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4776; Vector Backbone:pFASTBAC1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_37149 Copy
Species: Drosophila melanogaster
Genetic Insert: E2F1-P!P3A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D-TOPO; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19081076
Proper citation: RRID:Addgene_37142 Copy
Species: Homo sapiens
Genetic Insert: vang-like 2
Vector Backbone Description: Backbone Size:4224; Vector Backbone:pAdLox GI 1488684; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16791850
Comments: contains EGFP hVangl2 WT
Proper citation: RRID:Addgene_37257 Copy
Species: Homo sapiens
Genetic Insert: Scribble
Vector Backbone Description: Backbone Size:4224; Vector Backbone:pAdLox GI 1488684; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16791850
Comments: contains EGFP C-term
Proper citation: RRID:Addgene_37256 Copy
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