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Species: Other
Genetic Insert: LF82_315
Vector Backbone Description: Backbone Size:4502; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29762554
Proper citation: RRID:Addgene_100505 Copy
Species: Other
Genetic Insert: LF82_252
Vector Backbone Description: Backbone Size:4502; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29762554
Proper citation: RRID:Addgene_100504 Copy
Species: Other
Genetic Insert: Lifeact-Citrine
Vector Backbone Description: Vector Backbone:pMpGWB403; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27703856
Proper citation: RRID:Addgene_100582 Copy
Species: Other
Genetic Insert: LF82_468
Vector Backbone Description: Backbone Size:4502; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29762554
Proper citation: RRID:Addgene_100502 Copy
Species: Other
Genetic Insert: LF82_786
Vector Backbone Description: Backbone Size:4502; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29762554
Proper citation: RRID:Addgene_100500 Copy
Species: Other
Genetic Insert: PuroR
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: Puromycin resistance gene ecnoding a puromycin N-acetyl-transferase.
Proper citation: RRID:Addgene_100682 Copy
Species: Other
Genetic Insert: SV40-NLS
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: N-terminal nuclear localization signal of SV40 large T antigen. Peptide tag for targeting the protein to the nucleus.
Proper citation: RRID:Addgene_100684 Copy
Species: Other
Genetic Insert: IRES2
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: IRES - internal ribosome entry site. ATG in included
Proper citation: RRID:Addgene_100702 Copy
Species: Other
Genetic Insert: Lentiviral 3' LTR
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: 3'LTR for lentivial vectors. The 3'LTR included the�woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) which stabilised the RNA, and the�self-inactivating 3' long terminal repeat (LTR) from HIV-1 (3' LTR (?U3))
IMPORT NOTES: Special character found in field(Comments)
Proper citation: RRID:Addgene_100703 Copy
Species: Other
Genetic Insert: SV40-ORI
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: SV40 origin of replication.
Proper citation: RRID:Addgene_100679 Copy
Species: Other
Genetic Insert: IRES2
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: IRES2 internal ribosome entry sequence
Proper citation: RRID:Addgene_100673 Copy
Species: Other
Genetic Insert: SV40 promoter
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: SV40 constitutive promoter
Proper citation: RRID:Addgene_100674 Copy
Species: Other
Genetic Insert: P2A
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: p2A (self-cleaving 2A peptide)
Proper citation: RRID:Addgene_100675 Copy
Species: Other
Genetic Insert: Nix-ActA
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_100758 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20418874
Comments: This plasmid is also known as pAAV.GFA104.PI.eGFP.WPRE.bGH (p2380) from the Penn Vector Core.
Proper citation: RRID:Addgene_100896 Copy
Species: Other
Genetic Insert: YPet(short)-FL-mCherry
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6238; Vector Backbone:pLPCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28945706
Proper citation: RRID:Addgene_101170 Copy
Species: Other
Genetic Insert: PCP-GFP
Vector Backbone Description: Vector Backbone:plenti; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28290446
Proper citation: RRID:Addgene_101161 Copy
Species: Other
Genetic Insert: GPI
Vector Backbone Description: Backbone Size:5151; Vector Backbone:pACP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Originally cloned by Covalys. The GPI only is 87 bp. The size of ER signal sequence + ACPwt+GPI is 408 bp. The ACP tag is N-terminal to the GPI sequence. Additional resistance to Kanamycin
For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/
This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
Proper citation: RRID:Addgene_101131 Copy
Species: Other
Genetic Insert: YPet(short)-F40-mCherry
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6238; Vector Backbone:pLPCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26523364
Proper citation: RRID:Addgene_101252 Copy
Species: Other
Genetic Insert: PP-BRD3
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26092); Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: cgd4_1340:MAC04H-E04:C228715. SGC or PDB link: http://www.thesgc.org/structures/5U9N/
Proper citation: RRID:Addgene_101255 Copy
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