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Vector Backbone Description: Backbone Marker:Malcolm Moore lab; Vector Backbone:pUltra-Chili; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34088832
Comments: See Addgene plasmid #24129 for cloning details.
Proper citation: RRID:Addgene_48688 Copy
Species: Homo sapiens
Genetic Insert: Lacritin
Vector Backbone Description: Backbone Marker:New England BioLabs, INC; Backbone Size:7477; Vector Backbone:pTYB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23640897
Comments: Please note that amino acid numbering of this plasmid is relative to amino acid #20 in GenBank reference sequence NP_150593.1. E20 in the reference sequence is considered the first amino acid of lacritin in this plasmid.
Proper citation: RRID:Addgene_48721 Copy
Vector Backbone Description: Backbone Marker:Malcolm Moore lab; Backbone Size:8300; Vector Backbone:pUltra; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: See Addgene plasmid #24129 for cloning details.
Proper citation: RRID:Addgene_48687 Copy
Species: Homo sapiens
Genetic Insert: Lacritin
Vector Backbone Description: Backbone Marker:New England BioLabs, INC; Backbone Size:7477; Vector Backbone:pTYB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23640897
Comments: Please note that amino acid numbering of this plasmid is relative to amino acid #20 in GenBank reference sequence NP_150593.1. E20 in the reference sequence is considered the first amino acid of lacritin in this plasmid.
Lacritin protein sequence in this plasmid is: SILLTEQALAKAGKGMHGGVPGGKQFIENGSEFAQKLLKKFSLLKPWA
Proper citation: RRID:Addgene_48720 Copy
Species: Synthetic
Genetic Insert: AmCyan
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4000; Vector Backbone:pmR-mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: the nAmCyan and the mCherry genes are separated by a P2A sequence that results in 2 separate proteins. It also includes BsmBI restriction sites flanking the nAmCyan gene which result in BsiWI and BssHII overhangs for cloning in-frame with P2A-mCherry.
Proper citation: RRID:Addgene_48686 Copy
Species: Homo sapiens
Genetic Insert: Lacritin
Vector Backbone Description: Backbone Marker:New England BioLabs, INC; Backbone Size:7477; Vector Backbone:pTYB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23640897
Comments: Please note that amino acid numbering of this plasmid is relative to amino acid #20 in GenBank reference sequence NP_150593.1. E20 in the reference sequence is considered the first amino acid of lacritin in this plasmid.
Proper citation: RRID:Addgene_48725 Copy
Species: Homo sapiens
Genetic Insert: Rab8a
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25313408
Proper citation: RRID:Addgene_101050 Copy
Species: Homo sapiens
Genetic Insert: KDM4A
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26526725
Proper citation: RRID:Addgene_101052 Copy
Species: Homo sapiens
Genetic Insert: KMT2B homology arms
Vector Backbone Description: Backbone Marker:Eric Mendenhall & Richard M. Myers (Addgene plasmid # 63934); Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26355004
Comments: Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_101070 Copy
Genetic Insert: BDKRB2-dCas9(N)
Vector Backbone Description: Vector Backbone:pTMt_NES_TCS(Q’G)_HA-dCas9( N)_P2A-Puro-WPRE; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28903044
Proper citation: RRID:Addgene_101108 Copy
Species: Rhodopseudomonas palustris CGA009
Genetic Insert: ppsR2
Vector Backbone Description: Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29091422
Proper citation: RRID:Addgene_101068 Copy
Genetic Insert: TMt-dCas9(N)
Vector Backbone Description: Vector Backbone:pX855; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28903044
Proper citation: RRID:Addgene_101101 Copy
Species: Homo sapiens
Genetic Insert: RERE homology arms
Vector Backbone Description: Backbone Marker:Eric Mendenhall & Richard M. Myers (Addgene plasmid # 63934); Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26355004
Comments: Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_101069 Copy
Genetic Insert: VEGFR2-dCas9(N)
Vector Backbone Description: Vector Backbone:pTMt_NES_TCS(Q’G)_HA-dCas9(N)_P2A-Puro-WPRE; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28903044
Proper citation: RRID:Addgene_101104 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RP51A
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28829039
Proper citation: RRID:Addgene_100988 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RP51A
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28829039
Proper citation: RRID:Addgene_100989 Copy
Species: Homo sapiens
Genetic Insert: CMV
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4.18; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21653923
Proper citation: RRID:Addgene_100984 Copy
Vector Backbone Description: Backbone Marker:Feng Zhang lab; Backbone Size:8506; Vector Backbone:eSpCas9(1.1); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_101039 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RP51A
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28829039
Proper citation: RRID:Addgene_100986 Copy
Species: Homo sapiens
Genetic Insert: NR0B1
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4.18; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21653923
Proper citation: RRID:Addgene_100983 Copy
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