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Species: Schizosaccharomyces pombe
Genetic Insert: tRNA promoter : sgRNA cassette
Vector Backbone Description: Backbone Size:4988; Vector Backbone:pLSB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33313420
Comments: The following acknowledgement must be included in all research publications and other outputs: “pLSB-NAT was created with the support of the Wellcome Trust [095021], [200885], [203149]”.
The following statement must be included on all submissions of original research to peer-reviewed journals: “pLSB-NAT was created with the support of the Wellcome Trust [095021], [200885], [203149]”.
Proper citation: RRID:Addgene_166698 Copy
Species: Synthetic
Genetic Insert: dCas12a-NLS
Vector Backbone Description: Backbone Size:7206; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34197613
Proper citation: RRID:Addgene_166731 Copy
Species: HSV-1
Genetic Insert: UL8 and UL29 gRNA
Vector Backbone Description: Vector Backbone:pCCL-PGK-egfp; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33432198
Proper citation: RRID:Addgene_166685 Copy
Species: Mus musculus
Genetic Insert: GFP-MIOX
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34591448
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.30.428974v1 for BioRxiv preprint
Proper citation: RRID:Addgene_166683 Copy
Species: Mus musculus
Genetic Insert: Kitl
Vector Backbone Description: Vector Backbone:pX458; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30504842
Proper citation: RRID:Addgene_166721 Copy
Species: Mus musculus
Genetic Insert: VP2C-GFP-MIOX
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34591448
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.30.428974v1 for BioRxiv preprint
Proper citation: RRID:Addgene_166682 Copy
Species: Mus musculus
Genetic Insert: MIOX
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34591448
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.30.428974v1 for BioRxiv preprint
Proper citation: RRID:Addgene_166681 Copy
Species: Mus musculus
Genetic Insert: VP2C-MIOX
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34591448
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.30.428974v1 for BioRxiv preprint
Proper citation: RRID:Addgene_166680 Copy
Species: synthetic/engineered
Genetic Insert: muGFP
Vector Backbone Description: Backbone Size:5405; Vector Backbone:pAM-DCA; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29330459
Proper citation: RRID:Addgene_166727 Copy
Genetic Insert: Non-targeting guide
Vector Backbone Description: Vector Backbone:Lenti-CRISPR-V2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33101674
Proper citation: RRID:Addgene_166725 Copy
Vector Backbone Description: Vector Backbone:BPK3082; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32483191
Proper citation: RRID:Addgene_166724 Copy
Species: Homo sapiens
Genetic Insert: Mcl-1
Vector Backbone Description: Vector Backbone:pLVX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35442739
Comments: 2nd generation lentiviral transfer plasmid that can be used to create lentivirus, for CMV driven expression of mCerulean3 fused to the N-terminus of Mcl-1. There is a flexible, 17 amino acid linker between mCerulean3 and Mcl-1. proteins.
Proper citation: RRID:Addgene_166752 Copy
Species: Mus musculus
Genetic Insert: Bcl-W
Vector Backbone Description: Vector Backbone:FUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35442739
Comments: 3rd generation lentiviral transfer plasmid that can be used to create lentivirus, for hUbC-driven gene expression of mCerulean3-fused to the N-terminus of Bcl-W. There is a 7 aa linker between mCerulean3 and Bcl-W.
Proper citation: RRID:Addgene_166751 Copy
Species: Synthetic
Genetic Insert: Venus
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35442739
Comments: Plasmid can be used to express monomeric Venus fluorophore in bacteria. We used E.coli BL21-AI (Arabinose induction) for protein production, induced 5h at 30oC. Purify protein via Nickel column chromatography. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166756 Copy
Species: Homo sapiens
Genetic Insert: NLRP1
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33731932
Comments: Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity
Proper citation: RRID:Addgene_166813 Copy
Species: Homo sapiens
Genetic Insert: NLRP1
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33731932
Comments: Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity
Proper citation: RRID:Addgene_166812 Copy
Species: Homo sapiens
Genetic Insert: DPP9
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33731932
Comments: Mammalian cell expression vector for FLAG-tagged DPP9S. E597R mutant is partial loss of CARD8 binding.
Proper citation: RRID:Addgene_166800 Copy
Species: Adeno-associated virus
Genetic Insert: AAV2 rep/AAV11 cap
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pSP72; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15567432
Comments: Adenovirus VA, E4, and E2a genes are also present on the backbone.
Proper citation: RRID:Addgene_166888 Copy
Species: Homo sapiens
Genetic Insert: NLRP1
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33731932
Comments: Mammalian cell expression vector for FLAG-tagged NLRP1. The S1213A mutant is deficient in FIIND autoprocessing
Proper citation: RRID:Addgene_166804 Copy
Species: Homo sapiens
Genetic Insert: DPP9
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33731932
Comments: Mammalian cell expression vector for FLAG-tagged DPP9S. The D808N mutant is catalytically dead.
Proper citation: RRID:Addgene_166802 Copy
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