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Species: Homo sapiens
Genetic Insert: DPP9
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33731932
Comments: Mammalian cell expression vector for FLAG-tagged DPP9S. The S730A mutant is catalytically dead.
Proper citation: RRID:Addgene_166801 Copy
Species: Homo sapiens
Genetic Insert: NLRP1
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33731932
Comments: Mammalian cell expression vector for FLAG-tagged NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity
Proper citation: RRID:Addgene_166808 Copy
Species: Homo sapiens
Genetic Insert: NLRP1
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33731932
Comments: Mammalian cell expression vector for FLAG-tagged NLRP1. The LL1193E mutant abolishes FL-NLRP1 association with DPP9 at interface I.
Please note: Plasmid contains a H763N mutation in NLRP1. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_166805 Copy
Species: Homo sapiens
Genetic Insert: DPP8
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33731932
Comments: Mammalian cell expression vector for Flag-tagged DPP8
Proper citation: RRID:Addgene_166793 Copy
Species: Homo sapiens
Genetic Insert: NLRP1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33731932
Comments: Mammalian codon-optimized NLRP1 with N-terminal and C-terminal HIS tags
Proper citation: RRID:Addgene_166833 Copy
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6D; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33731932
Comments: Modified LIC 6D backbone with a C-terminal TEV-GFP-FLAG tag. Derived from Scott Gradia's pcDNA3.1 LIC 6D by Liron David.
Proper citation: RRID:Addgene_166835 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VAC8
Vector Backbone Description: Vector Backbone:pRS405; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508216
Comments: Linearize with AflII
Proper citation: RRID:Addgene_166838 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATG5 ATG12 ATG16
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26812546
Proper citation: RRID:Addgene_166851 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Atg13[571–700]
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31352862
Proper citation: RRID:Addgene_166855 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Atg13[571–700]
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31352862
Proper citation: RRID:Addgene_166854 Copy
Species: Xenopus laevis
Genetic Insert: Sizzled
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9428410
Comments: This encodes a secreted
antagonist of Xwnt-8.
The entire open reading frame of sizzled was amplified by low-cycle number PCR using Pfu polymerase and cloned as a
BamHI-XhoI fragment into pCS2+ .
Proper citation: RRID:Addgene_16688 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Atg13[571–700]
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31352862
Proper citation: RRID:Addgene_166852 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VAC8
Vector Backbone Description: Vector Backbone:pRS405; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508216
Comments: Linearize with AflII
Proper citation: RRID:Addgene_166840 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VAC8
Vector Backbone Description: Vector Backbone:pRS405; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508216
Comments: Linearize with AflII
Proper citation: RRID:Addgene_166844 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VAC8
Vector Backbone Description: Vector Backbone:pRS405; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508216
Comments: Linearize with AflII
Proper citation: RRID:Addgene_166841 Copy
Species: Homo sapiens
Genetic Insert: Dkk1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:xenopus/mammalian/avian/zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11448771
Comments: This is wild-type, full-length Dickkopf-1, Flag tagged.
Proper citation: RRID:Addgene_16690 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATG1
Vector Backbone Description: Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30527663
Comments: Linearize with StuI
Proper citation: RRID:Addgene_166847 Copy
Species: Synthetic
Genetic Insert: U6-VEGFA array presgRNA
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc.; Backbone Size:3315; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34178991
Proper citation: RRID:Addgene_166873 Copy
Species: Synthetic
Genetic Insert: U6-VEGFA presgRNA
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc.; Backbone Size:3316; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34178991
Proper citation: RRID:Addgene_166872 Copy
Species: Synthetic
Genetic Insert: U6-control presgRNA
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc.; Backbone Size:3316; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34178991
Proper citation: RRID:Addgene_166871 Copy
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