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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pPTK011-3a-SA-αMFΔ Resource Report Resource Website |
RRID:Addgene_84970 | Serum albumin followed by αMFΔ secretion tag | Synthetic | Chloramphenicol | PMID:28252957 | Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061) | Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:21:20 | 0 | |
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pPTK003-2-pENO1 Resource Report Resource Website |
RRID:Addgene_84962 | Enolase 1 promoter | Synthetic | Chloramphenicol | PMID:28252957 | Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061) | Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | BsaI site removed (2350 g to c) and BsmBI site removed (2424 g to c) | 2026-08-15 01:21:24 | 0 |
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pPTK005-3a-αMF Resource Report Resource Website |
RRID:Addgene_84964 | α-mating factor secretion tag | Synthetic | Chloramphenicol | PMID:28252957 | Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061) | Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:21:20 | 0 | |
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pPTK004-2-pTPI1 Resource Report Resource Website |
RRID:Addgene_84963 | Triose phosphate isomerase 1 promoter | Synthetic | Chloramphenicol | PMID:28252957 | Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061) | Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:21:20 | 0 | |
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pPTK006-3a-αMF no EAEA Resource Report Resource Website |
RRID:Addgene_84965 | α-mating factor no EAEA secretion tag | Synthetic | Chloramphenicol | PMID:28252957 | Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061) | Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:21:25 | 0 | |
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pPTK009-3a-αAmylase-αMFΔ Resource Report Resource Website |
RRID:Addgene_84968 | α-Amylase followed by MFΔ secretion tag | Synthetic | Chloramphenicol | PMID:28252957 | Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061) | Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:21:25 | 0 | |
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pPTK010-3a-Glucoamylase-αMFΔ Resource Report Resource Website |
RRID:Addgene_84969 | Glucoamylase followed by αMFΔ secretion tag | Synthetic | Chloramphenicol | PMID:28252957 | Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061) | Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:21:20 | 0 | |
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pPTK021-7-PARS Resource Report Resource Website |
RRID:Addgene_84990 | Pichia autonomously replicating sequence | Synthetic | Chloramphenicol | PMID:28252957 | Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061) | Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:21:25 | 0 | |
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pPTK019-4-tAOX1 Resource Report Resource Website |
RRID:Addgene_84988 | Alcohol oxidase 1 terminator | Synthetic | Chloramphenicol | PMID:28252957 | Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061) | Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:21:20 | 0 | |
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pPTK020-7-attB Resource Report Resource Website |
RRID:Addgene_84989 | BxbI recognition site | Synthetic | Chloramphenicol | PMID:28252957 | Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061) | Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | BsaI site removed (1711 t to a) | 2026-08-15 01:21:20 | 0 |
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pPdX Resource Report Resource Website 1+ mentions |
RRID:Addgene_85084 | Putidaredoxin | Synthetic | Kanamycin | PMID:27781345 | The PdX gene was subcloned from a synthetic gene whose codons are optimized for E. coli expression. | Backbone Marker:Merck Millipore; Backbone Size:5333; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | changed Cysteine 73 to Serine and Cysteine 85 to Serine | 2026-08-15 01:21:26 | 1 |
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pPdR Resource Report Resource Website |
RRID:Addgene_85083 | Putidaredoxin reductase | Synthetic | Kanamycin | PMID:27781345 | The PdR gene was subcloned from a synthetic gene whose codons are optimized for E. coli expression. | Backbone Marker:Merck Millipore; Backbone Size:5333; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:21:21 | 0 | |
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pR1G108CP20X Resource Report Resource Website |
RRID:Addgene_85086 | The G108C variant of PCNA1 fused to putidaredoxin reductase and putidaredoxin | Synthetic | Kanamycin | PMID:27781345 | A synthetic gene of PdR was inserted at the NdeI site of p1G108CP20X (Plasmid #85096) using In-Fusion cloning kit. | Backbone Marker:Merck Millipore; Backbone Size:5332; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | G108C in PCNA1 | 2026-08-15 01:21:21 | 0 |
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pC3 Resource Report Resource Website |
RRID:Addgene_85087 | PCNA3 fused to P450cam | Synthetic | Kanamycin | PMID:27781345 | The plamid was obtained from pGS-3 (Plasmid #85094) and pBsaP450camGG (Plasmid #85101) by Golden Gate assembly. | Backbone Marker:Merck Millipore; Backbone Size:5332; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:21:26 | 0 | |
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p3R112C/T180C-GG Resource Report Resource Website |
RRID:Addgene_85093 | DNA polymerase sliding clamp 3 | Synthetic | Kanamycin | PMID:27781345 | The PCNA3 gene was subcloned from a synthetic gene whose codons are optimized for E. coli expression. | Backbone Marker:Merck Millipore; Backbone Size:5329; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | changed Arginine 112 to Cysteine and Threonine 180 to Cysteine | 2026-08-15 01:21:21 | 0 |
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pGS-3 Resource Report Resource Website |
RRID:Addgene_85094 | DNA polymerase sliding clamp 3 | Synthetic | Kanamycin | PMID:27781345 | The PCNA3 gene was subcloned from a synthetic gene whose codons are optimized for E. coli expression. | Backbone Marker:Merck Millipore; Backbone Size:5332; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:21:21 | 0 | |
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pHPro52 Resource Report Resource Website |
RRID:Addgene_85098 | Pro-Pro-Pro-Pro-Pro | Synthetic | Chloramphenicol | PMID:27781345 | Backbone Marker:TaKaRa; Backbone Size:2210; Vector Backbone:pHSG398; Vector Types:Cloning vector; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:21:26 | 0 | ||
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pBsaPro5X Resource Report Resource Website |
RRID:Addgene_85100 | Putidaredoxin | Synthetic | Chloramphenicol | PMID:27781345 | A synthetic gene of PdX was inserted between EcoRI and BamHI sites of pHSG398 using In-Fusion cloning kit. | Backbone Marker:TaKaRa; Backbone Size:2210; Vector Backbone:pHSG398; Vector Types:Cloning vector; Bacterial Resistance:Chloramphenicol | changed Cysteine 73 to Serine and Cysteine 85 to Serine | 2026-08-15 01:21:26 | 0 |
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pAAV-hSyn-FLEX-TVA-P2A-EGFP-2A-oG Resource Report Resource Website 10+ mentions |
RRID:Addgene_85225 | Optimized G Protein | Synthetic | Ampicillin | Vector Backbone:pAAV-syn-FLEX-splitTVA-eGFP-B19G IW; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin | 2026-08-15 01:21:28 | 14 | |||
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LeGO-EBFP2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_85213 | EBFP2 | Synthetic | Ampicillin | PMID:28109958 | HIV-1 derived third generation lentiviral vector for stable cell marking. Use second generation (like psPAX2) or third generation (like pMDLg/pRRE + pRSV-Rev) systems for packaging in addition to a VSV-G expressing plasmid (or another envelope protein). Please visit the LeGO-Vector home page for more information: http://www.LentiGO-Vectors.de The BsrGI site of EBFP2 can be used to fuse an antibiotic resistance to the fluorescent protein following the building block principle of the LeGO vector system. More information is available on the LeGO website and in the following publications. More references: Weber et al., Molecular Therapy, 2008: http://www.nature.com/mt/journal/v16/n4/full/mt20086a.html Weber et al., Gene Therapy, 2010: http://www.nature.com/gt/journal/v17/n4/full/gt2009149a.html | Backbone Size:6417; Vector Backbone:LeGO vector; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin | 2026-08-15 01:21:23 | 4 |
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