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Genetic Insert: Histone, 2A element, rabies B19 glycoprotein
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Comments: Please note that there are 2 mutations in Histone, D26G and V119I. These mutations should not affect plasmid function.
Proper citation: RRID:Addgene_37452 Copy
Species: E. coli
Genetic Insert: CsrA
Vector Backbone Description: Backbone Marker:Lim Lab; Vector Backbone:pZ with p15a origin; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23878244
Proper citation: RRID:Addgene_37571 Copy
Species: HPV
Genetic Insert: HPV 5E6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:9387; Vector Backbone:pLenti 6.3/V5 DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22553330
Proper citation: RRID:Addgene_37449 Copy
Species: HPV
Genetic Insert: HPV 16E6no*
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:9387; Vector Backbone:pLenti 6.3/V5 DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22553330
Proper citation: RRID:Addgene_37445 Copy
Species: A. victoria
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Lim Lab; Vector Backbone:pZ with ColE origin; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23878244
Proper citation: RRID:Addgene_37566 Copy
Species: HPV
Genetic Insert: HPV 6bE6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:9387; Vector Backbone:pLenti 6.3/V5 DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22553330
Proper citation: RRID:Addgene_37447 Copy
Species: Homo sapiens
Genetic Insert: MET
Vector Backbone Description: Backbone Marker:Eric Campeau Addgene Plasmid 17448; Backbone Size:8608; Vector Backbone:pLentiCMV GFP Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_37561 Copy
Species: Homo sapiens
Genetic Insert: MET
Vector Backbone Description: Backbone Marker:Eric Campeau Addgene Plasmid 17448; Backbone Size:8608; Vector Backbone:pLentiCMV GFP Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_37560 Copy
Species: N/A
Genetic Insert: PreScission protease cleavage site-S tag-TEV site-Z
Vector Backbone Description: Backbone Size:6515; Vector Backbone:pSY07; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22431751
Proper citation: RRID:Addgene_37558 Copy
Species: HPV
Genetic Insert: HPV 8E6
Vector Backbone Description: Backbone Size:7500; Vector Backbone:pB-actin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22553330
Proper citation: RRID:Addgene_37438 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Met3p-mCherry-Tub1
Vector Backbone Description: Backbone Size:4381; Vector Backbone:pRS306; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21294277
Proper citation: RRID:Addgene_37554 Copy
Species: jellyfish Aequorea victoria
Genetic Insert: Venus N-term (aa 1-272)
Vector Backbone Description: Backbone Size:4381; Vector Backbone:pRS306; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21294277
Proper citation: RRID:Addgene_37555 Copy
Species: HPV
Genetic Insert: HPV 11E6
Vector Backbone Description: Backbone Size:7500; Vector Backbone:pB-actin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22553330
Proper citation: RRID:Addgene_37436 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ras2 C-term (last 9 aa)
Vector Backbone Description: Backbone Size:4749; Vector Backbone:pKT0128; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19755860
Proper citation: RRID:Addgene_37557 Copy
Species: Discosoma sp.
Genetic Insert: 3mCherry
Vector Backbone Description: Backbone Size:4271; Vector Backbone:pRS304; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19185494
Proper citation: RRID:Addgene_37550 Copy
Species: Discosoma sp.
Genetic Insert: 3mCherry
Vector Backbone Description: Backbone Size:5504; Vector Backbone:pRS305; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19185494
Proper citation: RRID:Addgene_37552 Copy
Species: Homo sapiens
Genetic Insert: Huwe1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2717; Vector Backbone:pENTR1A; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Comments: Full-length cDNA constructed by sequential cloning of PCR products and adding to a partial cDNA.
The ORF sequence provided contains Q2315H and 3016del3031 amino acid mutations compared to closest reference sequence NP_113584.3).
Proper citation: RRID:Addgene_37432 Copy
Species: Caenorhabditis elegans
Genetic Insert: GLH-1::nos-2 3’UTR
Vector Backbone Description: Backbone Marker:custom; Backbone Size:6700; Vector Backbone:pEV1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20335358
Proper citation: RRID:Addgene_37487 Copy
Genetic Insert: TetR
Vector Backbone Description: Backbone Marker:Lim Lab; Vector Backbone:pZ with ColE origin; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23878244
Proper citation: RRID:Addgene_37637 Copy
Species: Homo sapiens
Genetic Insert: NLRC5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22490867
Comments: The iso3 plasmid actually encodes the full cDNA (not only the ORF).
In comparison to the FL NLRC5, iso3 contains an insert that leads to a stop codon according to IPI00647281.1 .
Proper citation: RRID:Addgene_37510 Copy
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