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Species: Tobacco Rattle Virus
Genetic Insert: p35S:TRV2_Spacer_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
Vector Backbone Description: Vector Backbone:pEE515; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32483329
Proper citation: RRID:Addgene_149290 Copy
Species: Tobacco Rattle Virus
Genetic Insert: p35S:TRV2_NbAGsgRNA1_mFT:tNos
Vector Backbone Description: Vector Backbone:pEE083; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32483329
Proper citation: RRID:Addgene_149286 Copy
Species: Tobacco Rattle Virus
Genetic Insert: p35S:TRV2_NbAGsgRNA1_TrunFT:tNos
Vector Backbone Description: Vector Backbone:pEE083; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32483329
Proper citation: RRID:Addgene_149287 Copy
Species: Tobacco Rattle Virus
Genetic Insert: p35S:TRV2_NbAGsgRNA2_TrunFT:tNos
Vector Backbone Description: Vector Backbone:pEE515; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32483329
Proper citation: RRID:Addgene_149289 Copy
Species: Synthetic
Genetic Insert: SENP_EuB protease
Vector Backbone Description: Backbone Marker:Görlich lab; Backbone Size:3997; Vector Backbone:pDG-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31023724
Proper citation: RRID:Addgene_149333 Copy
Species: E. coli
Genetic Insert: Biotin ligase BirA
Vector Backbone Description: Backbone Marker:Görlich lab; Backbone Size:4278; Vector Backbone:pDG-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26633879
Proper citation: RRID:Addgene_149334 Copy
Species: Other
Genetic Insert: mcherry
Vector Backbone Description: Backbone Size:2218; Vector Backbone:puc19; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33499763
Proper citation: RRID:Addgene_149345 Copy
Species: Synthetic
Genetic Insert: LuxR-GFP
Vector Backbone Description: Vector Backbone:Unspecified; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34125913
Proper citation: RRID:Addgene_149472 Copy
Species: Synthetic
Genetic Insert: NahR(AM)-GFP
Vector Backbone Description: Vector Backbone:Unspecified; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34125913
Proper citation: RRID:Addgene_149473 Copy
Species: Synthetic
Genetic Insert: VanR(AM)-GFP
Vector Backbone Description: Vector Backbone:Unspecified; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34125913
Proper citation: RRID:Addgene_149477 Copy
Species: Synthetic
Genetic Insert: sgRNAflaB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2_2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149631 Copy
Species: Synthetic
Genetic Insert: sgRNArodA
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2_2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149635 Copy
Species: Synthetic
Genetic Insert: Psyn-mCherryBb
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2_2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149636 Copy
Species: Synthetic
Genetic Insert: dCas9, lacI
Vector Backbone Description: Vector Backbone:pCR2.1, heavily modified; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149637 Copy
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9K(RBSmut); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149577 Copy
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2_2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149613 Copy
Species: Homo sapiens
Genetic Insert: human frataxin cDNA
Vector Backbone Description: Backbone Marker:BMD Biosciences; Backbone Size:5400; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_14992 Copy
Species: Homo sapiens
Genetic Insert: PLAP
Vector Backbone Description: Backbone Size:0; Vector Backbone:pDOL; Vector Types:Mammalian Expression, Retroviral, Avian expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:1731342
Comments: Retroviral vector expressing PALP from the long-terminal-repeat (LTR) promoter. The neomycin gene is expressed from SV40 promoter.
Plasmid CMV-PLAP was digested with KpnI and a 1930bp fragment encoding PLAP was isolated. The ends of this fragment were blunted with T4 DNA polymerase and ligated to a SalI linker. The linker fragment was digested with SalI and inserted into the SalI site of the Moloney murine leukemia virus retroviral vector pDOL.
Proper citation: RRID:Addgene_15164 Copy
Species: Escherichia coli
Genetic Insert: beta-galactosidase
Vector Backbone Description: Backbone Size:0; Vector Backbone:modified pDOL; Vector Types:Mammalian Expression, Retroviral, Avian expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:3099292
Comments: Retroviral vector expressing beta-galactosidase from the long-terminal-repeat (LTR) promoter. The neomycin gene is expressed from SV40 promoter.
Proper citation: RRID:Addgene_15148 Copy
Species: Homo sapiens
Genetic Insert: ID1
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:p15A-PB; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Kanamycin
Comments: Genomic sequence subcloned by recombineering from a clone of RPCI-11 human genomic DNA library. The exact DNA sequence in this plasmid differs somewhat from the reference human genome sequence because the reference sequence is a composite sequence, i.e., the reference ID1 region sequence may not be derived from the RPCI-11 BAC clone utilized for this construct.
The ID1 intron was placed after G198, i.e., after about a third of the sequence encoding the active site of the cre protein. See https://doi.org/10.1073/pnas.2011448117 and https://doi.org/10.1093%2Fnar%2Fgkq384. Thus, without splicing, no catalytically active cre protein sequence is expected to be translated from this sequence.
Please make sure the plasmid DNA preparation is pure by gel electrophoresis before using.
Proper citation: RRID:Addgene_139280 Copy
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