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Species: Mus musculus
Genetic Insert: Cd36 Promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4411; Vector Backbone:pGL4.24; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31604271
Proper citation: RRID:Addgene_138578 Copy
Species: Mus musculus
Genetic Insert: mCherry:Mus musculus Prkd1(138-343)
Vector Backbone Description: Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32211893
Proper citation: RRID:Addgene_139314 Copy
Species: Mus musculus
Genetic Insert: TCRA
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:5357; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.30.470597v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_138983 Copy
Species: Mus musculus
Genetic Insert: TCRA
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:5357; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.30.470597v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_138989 Copy
Species: Mus musculus
Genetic Insert: SUMO1 GG
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17159996
Proper citation: RRID:Addgene_13990 Copy
Species: Mus musculus
Genetic Insert: PrP
Vector Backbone Description: Backbone Size:7200; Vector Backbone:pCB6+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11742063
Comments: Full length mouse PrP with 3F4 epitag was cloned into HindIII and BamHI sites of mammalian cell expression vector pCB6+.
Proper citation: RRID:Addgene_13917 Copy
Species: Mus musculus
Genetic Insert: CTCF CRISPR knock-in
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32203384
Proper citation: RRID:Addgene_139747 Copy
Species: Mus musculus
Genetic Insert: SUMO1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17159996
Proper citation: RRID:Addgene_13989 Copy
Species: Mus musculus
Genetic Insert: gRNA and GFP donor
Vector Backbone Description: Backbone Size:9288; Vector Backbone:pORANGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32275651
Proper citation: RRID:Addgene_139657 Copy
Species: Mus musculus
Genetic Insert: PI3K regulatory subunit p55gamma
Vector Backbone Description: Backbone Size:5441; Vector Backbone:pHM6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_1396 Copy
Species: Mus musculus
Genetic Insert: gRNA and GFP donor
Vector Backbone Description: Backbone Size:9285; Vector Backbone:pORANGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32275651
Proper citation: RRID:Addgene_139664 Copy
Species: Mus musculus
Genetic Insert: tandem HRS FYVE domain
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10970851
Proper citation: RRID:Addgene_140047 Copy
Species: Mus musculus
Genetic Insert: PI3K regulatory subunit p85beta
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Cantley lab recommends growth at room temperature.
Proper citation: RRID:Addgene_1408 Copy
Species: Mus musculus
Genetic Insert: myelocytomatosis oncogene-F2A-Kruppel-like factor 4-T2A-POU domain, class 5, transcription factor 1-E2A-SRY-box containing gene
Vector Backbone Description: Backbone Size:6397; Vector Backbone:pCAG-ires-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19252477
Proper citation: RRID:Addgene_20865 Copy
Species: Mus musculus
Genetic Insert: Notch 3 Intracellular Domain
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3XFLAG-CMV-7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16365048
Comments: All NICD plasmids were cloned into the p3XFLAG-CMV-7(TM) expression vector (Sigma). NICD3 was cloned into XbaI/BamHI sites. NICD3 (Met-1664 to Ala-2318 of full-length mouse N3) was amplified from the N3 plasmid (gift from Dr. U. Lendahl) with 5XbaN3 (5'-GCTCTAGAATGGTTGCCAGGCGAAAGCGAG-3') and 3BamN3
(5'-GCGGATCCCTAGGCCATCACCTGCCTCTTGGG-3') primers.
Proper citation: RRID:Addgene_20185 Copy
Species: Mus musculus
Genetic Insert: Notch 2 intracellular domain
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3XFLAG-CMV-7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16365048
Comments: All NICD plasmids were cloned into the p3XFLAG-CMV-7(TM) expression vector (Sigma). NICD2 was cloned into XbaI/BamHI sites. NICD2 (Met-1699 to Ala-2470 of full-length mouse N2) was amplified from the N2 plasmid (gift from Dr. Y. Hamada) using primers 5XbaN2 (5'-GCTCTAGAATGGCCAAGCGGAAGCGCAAG-3') and 3BamN2
(5'-GCGGATCCCTATGCATACACCTGCATGTTGCTG-3').
Please note that Addgene sequencing results confirm the updated domain numbering to be Met 1701-Ala 2473.
Proper citation: RRID:Addgene_20184 Copy
Species: Mus musculus
Genetic Insert: C-Fluc
Vector Backbone Description: Backbone Marker:Ohlfest Lab; Backbone Size:6082; Vector Backbone:colE1/ampR; Vector Types:Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19147555
Proper citation: RRID:Addgene_20203 Copy
Species: Mus musculus
Genetic Insert: pDEST27-Vinculin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8123; Vector Backbone:pDEST27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19075109
Proper citation: RRID:Addgene_20144 Copy
Species: Mus musculus
Genetic Insert: pCMV5 Flag p38 alpha
Vector Backbone Description: Backbone Marker:Dr. David Russell, UTSW; Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7535770
Proper citation: RRID:Addgene_20786 Copy
Species: Mus musculus
Genetic Insert: pDCNA3 Flag p38 delta
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10716930
Proper citation: RRID:Addgene_20785 Copy
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