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Species: Drosophila melanogaster
Genetic Insert: full length me31B
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29480805
Proper citation: RRID:Addgene_113001 Copy
Species: Drosophila melanogaster
Genetic Insert: full length gnu
Vector Backbone Description: Vector Backbone:pGEX6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28555567
Proper citation: RRID:Addgene_113000 Copy
Species: Drosophila melanogaster
Genetic Insert: full length gnu
Vector Backbone Description: Vector Backbone:pCaSpeR4; Vector Types:P insertion vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28555567
Proper citation: RRID:Addgene_113006 Copy
Species: Drosophila melanogaster
Genetic Insert: dhd
Vector Backbone Description: Vector Backbone:pCaSpeR4; Vector Types:P insertion vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113004 Copy
Species: Drosophila melanogaster
Genetic Insert: N terminus of tral
Vector Backbone Description: Vector Backbone:pMAL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29480805
Proper citation: RRID:Addgene_113009 Copy
Species: Drosophila melanogaster
Genetic Insert: C terminus of tral
Vector Backbone Description: Vector Backbone:pMAL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29480805
Proper citation: RRID:Addgene_113008 Copy
Species: Drosophila melanogaster
Genetic Insert: Kinesin 1-401 iLid His tag
Vector Backbone Description: Backbone Size:2500; Vector Backbone:pT7-7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31391558
Proper citation: RRID:Addgene_122484 Copy
Species: Drosophila melanogaster
Genetic Insert: U6:3-gRNA
Vector Backbone Description: Backbone Marker:Fillip Port; Vector Backbone:pCFD3-dU6-3gRNA (Addgene 49410); Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: When describing the use of this plasmid in publications, please state that it is derived from pCFD3 and cite:
Port et al. Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):E2967-76. doi: 10.1073/pnas.1405500111
In pCFD3.1, the BbsI sites have been mutated in mini-white, which allows use of the remaining BbsI sites for gRNA cloning (using the protocol for pCFD3 (https://www.crisprflydesign.org/plasmids/)).
Proper citation: RRID:Addgene_123366 Copy
Species: Drosophila melanogaster
Genetic Insert: Drosophila Rae1
Vector Backbone Description: Backbone Size:2563; Vector Backbone:pDONR221; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28554770
Proper citation: RRID:Addgene_124240 Copy
Species: Drosophila melanogaster
Genetic Insert: Drosophila Actin 5C
Vector Backbone Description: Backbone Size:2553; Vector Backbone:pDONR221; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_124242 Copy
Species: Drosophila melanogaster
Genetic Insert: wg gRNA
Vector Backbone Description: Backbone Size:7473; Vector Backbone:attB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34078888
Proper citation: RRID:Addgene_125006 Copy
Species: Drosophila melanogaster
Genetic Insert: dorsal RHR in pB1H2
Vector Backbone Description: Backbone Size:4093; Vector Backbone:pB1H2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16041365
Proper citation: RRID:Addgene_12613 Copy
Species: Drosophila melanogaster
Genetic Insert: Or47a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31504297
Comments: Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint
Proper citation: RRID:Addgene_126472 Copy
Species: Drosophila melanogaster
Genetic Insert: Or47a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31504297
Comments: Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint
Proper citation: RRID:Addgene_126473 Copy
Species: Drosophila melanogaster
Genetic Insert: DmelOrco
Vector Backbone Description: Backbone Size:4111; Vector Backbone:pCMV-BI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31504297
Comments: Is it recommended to digest the vector with XhoI and PvuI-HF and gel purify the two bands before sequencing as the two CMV promoters and SV40-polyA sequences are very similar to each other. Use the EcoRI restriction site for best functional expression on the MCS2. Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint
Proper citation: RRID:Addgene_126476 Copy
Species: Drosophila melanogaster
Genetic Insert: d-Dispatched1
Vector Backbone Description: Vector Backbone:pUAS; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29359685
Proper citation: RRID:Addgene_126412 Copy
Species: Drosophila melanogaster
Genetic Insert: Or47a
Vector Backbone Description: Backbone Size:4111; Vector Backbone:pCMV-BI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31504297
Comments: Is it recommended to digest the vector with XhoI and NaeI and gel purify the two bands before sequencing as the two CMV promoters and SV40-polyA sequences are very similar to each other Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint
Proper citation: RRID:Addgene_126478 Copy
Species: Drosophila melanogaster
Genetic Insert: Or56a
Vector Backbone Description: Backbone Size:4111; Vector Backbone:pCMV-BI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31504297
Comments: Is it recommended to digest the vector with XhoI and NaeI and gel purify the two bands before sequencing as the two CMV promoters and SV40-polyA sequences are very similar to each other Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint
Proper citation: RRID:Addgene_126479 Copy
Species: Drosophila melanogaster
Genetic Insert: LN-3xFlag
Vector Backbone Description: Vector Backbone:modified from pAcM; Vector Types:LamdaN entry vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31384064
Proper citation: RRID:Addgene_128012 Copy
Species: Drosophila melanogaster
Genetic Insert: Gal4-DBD-3xFlag
Vector Backbone Description: Vector Backbone:modified from pAcM; Vector Types:Gal4 entry vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31384064
Proper citation: RRID:Addgene_128014 Copy
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