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Species: Erwinia herbicola Eho10
Genetic Insert: crtE, crtB
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:13581; Vector Backbone:pAC-EHER; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:7919981
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53300 Copy
Species: Erwinia herbicola Eho10
Genetic Insert: crtE, idi, crtX, crtY, crtI, crtB, crtZ
Vector Backbone Description: Backbone Marker:New England BioLabs; Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:7919981
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53262 Copy
Species: E. coli
Genetic Insert: etpC
Vector Backbone Description: Backbone Size:3719; Vector Backbone:pACYC184; Vector Types:Bacterial Expression, pACYC184; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:19707189
Comments: Please note that there is a L to M mutation in etpC. This mutation should not affect function.
Proper citation: RRID:Addgene_53533 Copy
Species: e. coli
Genetic Insert: pLux
Vector Backbone Description: Backbone Size:1000; Vector Backbone:pLuxR; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24104480
Proper citation: RRID:Addgene_53544 Copy
Genetic Insert: None
Vector Backbone Description: Vector Backbone:pCAndy; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24550272
Proper citation: RRID:Addgene_53571 Copy
Species: Other
Genetic Insert: λCI-β-flap fusion
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:3142; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:9121589
Comments: Note-When cloning into this plasmid, the 5' end of the insert must provide an extra nucleotide to maintain the reading frame. Additionally, a stop codon must be inserted 5' to the BamHI site. Please see supplemental document for more details.
This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids:
http://www.addgene.org/browse/article/8393/
The following articles can provide more information on using the bacterial 2-hybrid system:
Dove, et al. 1997 PMID 9121589
Dove and Hochschild 1998 PMID 9499408
Deaconescu, et al. 2006 PMID 16469698
Deighan, et al. 2008 PMID 18832144
Kuznedelov, et al. 2002 PMID 11823642
Proper citation: RRID:Addgene_53733 Copy
Species: Synthetic
Genetic Insert: His10
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology, Bacillus BioBrick Box; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24295448
Comments: For sequencing of insert, use the following primers:
fwd: TGCCACCTGACGTCTAAG
rev: ATTACCGCCTTTGAGTGA
Proper citation: RRID:Addgene_55179 Copy
Species: Synthetic
Genetic Insert: cMyc tag
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology, Bacillus BioBrick Box; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24295448
Comments: For sequencing of insert, use the following primers:
fwd: TGCCACCTGACGTCTAAG
rev: ATTACCGCCTTTGAGTGA
Proper citation: RRID:Addgene_55183 Copy
Species: Synthetic
Genetic Insert: RFP cloning selection cassette
Vector Backbone Description: Backbone Marker:BioBricks Foundation; Backbone Size:2069; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26171760
Proper citation: RRID:Addgene_63674 Copy
Species: Other
Genetic Insert: LT gene probe
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:2254425
Comments: The LT probe is a 1-kilobase BamHI fragment derived from pWD299. The HincII fragment was removed from pWD299, BamHI linkers were attached, and this fragment was cloned into pACYC184
Proper citation: RRID:Addgene_63740 Copy
Species: Enteropathogenic Escherichia coli
Genetic Insert: eae
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:2172966
Proper citation: RRID:Addgene_63759 Copy
Species: Rhodobactor
Genetic Insert: crtEBI operon
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Comments: Needs to be grown in E_IF3_15 strain for overproduction of neurosporene
Proper citation: RRID:Addgene_63803 Copy
Species: Rhodobactor
Genetic Insert: crtEBI operon
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Comments: Needs to be grown in E_IF3_15 strain for overproduction of neurosporene
Proper citation: RRID:Addgene_63804 Copy
Species: Rhodobactor
Genetic Insert: crtEBI operon
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Comments: Needs to be grown in E_IF3_15 strain for overproduction of neurosporene
Proper citation: RRID:Addgene_63806 Copy
Species: Rhodobactor
Genetic Insert: crtEBI operon
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Comments: Needs to be grown in E_IF3_15 strain for overproduction of neurosporene
Proper citation: RRID:Addgene_63812 Copy
Species: Rhodobactor
Genetic Insert: crtEBI operon
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Comments: Needs to be grown in E_IF3_15 strain for overproduction of neurosporene
Proper citation: RRID:Addgene_63810 Copy
Species: Synthetic
Genetic Insert: mRFP1
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Proper citation: RRID:Addgene_63823 Copy
Species: Synthetic
Genetic Insert: mRFP1
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Proper citation: RRID:Addgene_63824 Copy
Species: Synthetic
Genetic Insert: mRFP1
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Proper citation: RRID:Addgene_63821 Copy
Species: Synthetic
Genetic Insert: mRFP1
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Proper citation: RRID:Addgene_63822 Copy
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