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Species: E. coli
Genetic Insert: E. coli type I-E Cas5
Vector Backbone Description: Backbone Size:5221; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34985758
Comments: If used in a publication, please acknowledge that the plasmid pEcCas5 was generated by U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility. The associated work was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.
Proper citation: RRID:Addgene_170090 Copy
Species: E. coli
Genetic Insert: E. coli type I-E Cse2
Vector Backbone Description: Backbone Size:5230; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34985758
Comments: If used in a publication, please acknowledge that the plasmid pEcCse2 was generated by U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility. The associated work was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.
Proper citation: RRID:Addgene_170094 Copy
Species: E. coli
Genetic Insert: SoxS
Vector Backbone Description: Vector Backbone:p15A; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34800362
Proper citation: RRID:Addgene_177323 Copy
Species: E. coli
Genetic Insert: dxs
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:0; Vector Backbone:pBluescript; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11180061
Proper citation: RRID:Addgene_17821 Copy
Species: E. coli
Genetic Insert: FLIPrbs
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14550551
Comments: A RBP PCR product from genomic E. coli DNA encoding mature RBP was cloned into the KpnI site of FLIPglu-600u in pRSETB, replacing the glucose sensor's mglB moiety. The RBP sequence was found to carry a D208G substitution, which does not significantly affect the binding affinity.
Proper citation: RRID:Addgene_17860 Copy
Species: E. coli
Genetic Insert: FLIPrbs T135A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14550551
Comments: T135A was generated using QuikChange (Stratagene) in the mutant background of FLIPrib-250n.
Proper citation: RRID:Addgene_17861 Copy
Species: E. coli
Genetic Insert: FLIPrbs F16A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14550551
Comments: F16A was generated using QuikChange (Stratagene) in the mutant background of FLIPrib-250n.
Proper citation: RRID:Addgene_17864 Copy
Species: E. coli
Genetic Insert: FLIPrbs R141A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14550551
Comments: R141A was generated using QuikChange (Stratagene) in the mutant background of FLIPrib-250n.
Proper citation: RRID:Addgene_17865 Copy
Species: E. coli
Genetic Insert: FLII12Pglu-700uDelta6
Vector Backbone Description: Backbone Size:0; Vector Backbone:pEF/myc/ER; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18177733
Proper citation: RRID:Addgene_17867 Copy
Species: E. coli
Genetic Insert: Escherichia coli RNase E residues 1-400
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:7280; Vector Backbone:pTYB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16854990
Proper citation: RRID:Addgene_17949 Copy
Species: E. Coli
Genetic Insert: ble
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: CDS
Proper citation: RRID:Addgene_165840 Copy
Species: E. Coli
Genetic Insert: nptII
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: CDS
Proper citation: RRID:Addgene_165836 Copy
Species: E. Coli
Genetic Insert: hph
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: CDS
Proper citation: RRID:Addgene_165839 Copy
Species: E. Coli
Genetic Insert: pac
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: CDS
Proper citation: RRID:Addgene_165837 Copy
Species: E. Coli
Genetic Insert: rrnBT1
Vector Backbone Description: Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: Bacterial Terminator
Proper citation: RRID:Addgene_165803 Copy
Species: E. Coli
Genetic Insert: rrnBT2
Vector Backbone Description: Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: Bacterial Terminator
Proper citation: RRID:Addgene_165804 Copy
Species: E. coli
Genetic Insert: RecT
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pTJV1Sc-rpoB; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35673614
Proper citation: RRID:Addgene_166983 Copy
Species: E. coli
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:The plasmid is built using multiple fragments; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:33608525
Proper citation: RRID:Addgene_174653 Copy
Species: E. Coli
Genetic Insert: MBP
Vector Backbone Description: Backbone Marker:Tobias Meyer (Addgene plasmid # 85132); Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35972904
Proper citation: RRID:Addgene_175103 Copy
Species: E. coli
Genetic Insert: beta-galactosidase with stuffer
Vector Backbone Description: Backbone Size:30000; Vector Backbone:pAdEasy-2; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9482916
Comments: beta-gal with stuffer (total 10kb) was cloned into pAdTrack-CMV, then recombined into pAdEasy-2.
AdEasy is a registered trademark of the Johns Hopkins University.
Proper citation: RRID:Addgene_16407 Copy
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