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Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Zhang lab; Vector Backbone:lentiCRISPR (pXPR_001), Addgene plasmid #49535; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24336571
Comments: gRNA target sequence GGAGCGCACCATCTTCTTCA
These six EGFP-targeting lentiCRISPRs have EGFP-targeting sgRNAs cloned into the lentiCRISPR backbone plasmid (Addgene plasmid 49535) and are used in Fig 1B of Shalem*, Sanjana* et al (2014). If you want to clone your own targeting sequences, please use the lentiCRISPR backbone plasmid (Addgene plasmid 49535), as the sgRNA Type IIs cloning site is not present in these plasmids.
Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check https://zlab.bio/ for the most up-to-date information.
Proper citation: RRID:Addgene_51763 Copy
Species: Synthetic
Genetic Insert: Sirius
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19349978
Comments: GenBank accession number: AB444952
Proper citation: RRID:Addgene_51957 Copy
Species: Synthetic
Genetic Insert: Sirius
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19349978
Comments: GenBank accession number: AB444952
Proper citation: RRID:Addgene_51956 Copy
Species: Synthetic
Genetic Insert: ATeam1.03-nD/nA
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pENTR1A; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20047338
Proper citation: RRID:Addgene_51959 Copy
Species: Synthetic
Genetic Insert: ATeam1.03-nD/nA
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20047338
Proper citation: RRID:Addgene_51960 Copy
Species: Synthetic
Genetic Insert: Yellow Cameleon-Nano140
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20693999
Comments: GenBank accession number: HM145949
Proper citation: RRID:Addgene_51966 Copy
Species: Synthetic
Genetic Insert: Yellow Cameleon-Nano15
Vector Backbone Description: Vector Backbone:pBig delta BamHI; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20693999
Comments: The vector pBIG is derived from the published construct pATANB43 (Dynes and Firtel, 1989). pBIG contains a 5.9 kb fragment of the native Dictyostelium extrachromosomal plasmid Ddpl that confers autonomous replication in Dictyostelium, a 2.2 kb actm 6 promoter-G418-resistant gene cartridge, and vector sequences from pBluescript (11) SK (Stratagene). (reference: Cell, Vol 75, Issue 2, 1993, 361-371).
Proper citation: RRID:Addgene_51962 Copy
Species: Synthetic
Genetic Insert: Yellow Cameleon-Nano15
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20693999
Comments: GenBank accession number: GU071083
The minor discrepancies between the QC and depositor sequence have no functional consequence.
Proper citation: RRID:Addgene_51961 Copy
Species: Synthetic
Genetic Insert: Yellow Cameleon-Nano30
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20693999
Comments: GenBank accession number: GU071084
Proper citation: RRID:Addgene_51963 Copy
Species: Synthetic
Genetic Insert: Nano-lantern
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23232392
Comments: GenBank accession number: JN859492. The improved brightness of Nano-lantern should generate power densities in the range of 1μW/cm2 (versus 0.1μW/cm2 for RLuc8) following transient overexpression in the micromolar range in human cells, and thus increase imaging potential.
Proper citation: RRID:Addgene_51969 Copy
Species: Synthetic
Genetic Insert: Nano-lantern
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23232392
Comments: GenBank accession number: JN859492
The minor discrepancies between the QC and depositor sequence have no functional consequence.
Proper citation: RRID:Addgene_51971 Copy
Species: Synthetic
Genetic Insert: Nano-lantern_CaM-2GS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23232392
Comments: GenBank accession number: JN859494
Proper citation: RRID:Addgene_51979 Copy
Species: Synthetic
Genetic Insert: Nano-lantern
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23232392
Comments: GenBank accession number: JN859492
Proper citation: RRID:Addgene_51972 Copy
Species: Synthetic
Genetic Insert: Nano-lantern
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23232392
Comments: GenBank accession number: JN859492
The minor discrepancies between the QC and depositor sequence have no functional consequence and Addgene's QC sequence should be used for comparison.
Please note the upstream KpnI site shown on the depositor's map is not present in this plasmid.
Proper citation: RRID:Addgene_51975 Copy
Species: Synthetic
Genetic Insert: Nano-lantern_CaM_E104Q-4GS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23232392
Comments: GenBank accession number: JN859498
Proper citation: RRID:Addgene_51980 Copy
Species: Synthetic
Genetic Insert: Nano-lantern_CaM_E104Q-2G
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23232392
Comments: GenBank accession number: JN859496
The minor discrepancies between the QC and depositor sequence have no functional consequence.
Proper citation: RRID:Addgene_51982 Copy
Species: Synthetic
Genetic Insert: Nano-lantern_CaM-4GS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23232392
Comments: GenBank accession number: JN859495
Proper citation: RRID:Addgene_51981 Copy
Species: Synthetic
Genetic Insert: Nano-lantern_ATP0
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23232392
Comments: GenBank accession number: JN974906. Although they differ by only three N-terminal amino acidsin the RLuc8-S257G moiety, their dynamic range and brightness were vastly different. Nano-lantern (ATP1) exhibited enough dynamic range (200%) for functional imaging, and was 1.5-fold brighter than Nano-lantern (ATP0) at ATP-saturating condition. They had similar affinities for ATP.
Proper citation: RRID:Addgene_51989 Copy
Species: Synthetic
Genetic Insert: Nano-lantern_CaM_E104Q-3GS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23232392
Comments: GenBank accession number: JN859497
The minor discrepancies between the QC and depositor sequence have no functional consequence.
Proper citation: RRID:Addgene_51983 Copy
Species: Synthetic
Genetic Insert: Nano-lantern_CaM_E104Q-4GS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23232392
Comments: GenBank accession number: JN859498
Proper citation: RRID:Addgene_51986 Copy
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