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Species: Homo sapiens
Genetic Insert: CRAF
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBABEpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_51126 Copy
Species: Homo sapiens
Genetic Insert: H2B-EGFP
Vector Backbone Description: Backbone Size:8344; Vector Backbone:pLenti.Syn(0.5).eGFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please note that the H2B sequence in this plasmid was derived from H2B-GFP (Addgene plasmid# 11680) and contains the same differences (D26G and V119I) compared to GenBank reference sequences, such as NP_066402.2.
Proper citation: RRID:Addgene_51004 Copy
Genetic Insert: H2B-ChFP
Vector Backbone Description: Backbone Size:6686; Vector Backbone:pRRLsin.cPPT.PGKp.eGFP.wpre; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26209648
Comments: Please note that the H2B sequence in this plasmid was derived from H2B-GFP (Addgene plasmid# 11680) and contains the same differences (D26G and V119I) compared to GenBank reference sequences, such as NP_066402.2.
Proper citation: RRID:Addgene_51007 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8089; Vector Backbone:pSIR; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25051498
Proper citation: RRID:Addgene_51128 Copy
Species: Homo sapiens
Genetic Insert: CRAF
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBABEpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_51127 Copy
Vector Backbone Description: Backbone Marker:Bert Vogelstein lab (Addgene Plasmid # 16405); Backbone Size:8935; Vector Backbone:pAdTrack-CMV; Vector Types:Adenoviral; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_50957 Copy
Species: Mus musculus
Genetic Insert: Gli2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pEF5-FRT-DEST; Vector Types:Mammalian Expression, Flpin system; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24373970
Proper citation: RRID:Addgene_51254 Copy
Species: Mus musculus
Genetic Insert: Gli2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pEF5-FRT-DEST; Vector Types:Mammalian Expression, Flpin system; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24373970
Proper citation: RRID:Addgene_51253 Copy
Species: Mus musculus
Genetic Insert: Gli2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pEF5-FRT-DEST; Vector Types:Mammalian Expression, Flpin system; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24373970
Proper citation: RRID:Addgene_51256 Copy
Species: Mus musculus
Genetic Insert: Gli2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pEF5-FRT-DEST; Vector Types:Mammalian Expression, Flpin system; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24373970
Proper citation: RRID:Addgene_51250 Copy
Species: Mus musculus
Genetic Insert: Gli2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pEF5-FRT-DEST; Vector Types:Mammalian Expression, Flpin system; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24373970
Proper citation: RRID:Addgene_51252 Copy
Species: Mus musculus
Genetic Insert: Gli2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pEF5-FRT-DEST; Vector Types:Mammalian Expression, Flpin system; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24373970
Proper citation: RRID:Addgene_51251 Copy
Genetic Insert: Co-InCreN
Vector Backbone Description: Backbone Marker:CLONTECH; Backbone Size:4814; Vector Backbone:pDIRECT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24413561
Comments: Please note that the complementary plasmid pCAG-Co-InCreC (www.addgene.org/51268) must be ordered with pCAG-Co-InCreN in order to successfully use the Co-Driver system as described in the associated publication.
Proper citation: RRID:Addgene_51267 Copy
Genetic Insert: b3STOPb3-ZsGreen
Vector Backbone Description: Backbone Marker:CLONTECH; Backbone Size:4814; Vector Backbone:pDIRECT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24413561
Proper citation: RRID:Addgene_51266 Copy
Species: Synthetic
Genetic Insert: 3xFLAG-dCas9
Vector Backbone Description: Backbone Marker:Hodaka Fujii; Backbone Size:6238; Vector Backbone:pMXs-neo; Vector Types:Mammalian Expression, Retroviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25051498
Comments: The coding sequence of 3xFLAG-dCas9 can be cleaved with Pac I and Not I.
Construction strategy of gRNA retroviral vectors
1. Cleave gBlock from a gRNA vector constructed using gRNA cloning vector (Addgene #41824) with appropriate restriction enzymes (eg. [Xho I + Hind III], EcoR I).
2. Insert the cleaved gBlock into pSIR-based self-inactivating retroviral vectors.
Vectors & Sites of insertion
pSIR-neo (Addgene #51128): eg. [Xho I + Hind III]
pSIR-GFP (Addgene #51134): eg. [Xho I + Hind III], EcoR I
pSIR-DsRed-Express2 (Addgene #51135): eg. [Xho I + Hind III], EcoR I
pSIR-hCD2 (Addgene #51143): eg. EcoR I
Proper citation: RRID:Addgene_51260 Copy
Species: Synthetic
Genetic Insert: Pyronic
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5419; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24465702
Proper citation: RRID:Addgene_51308 Copy
Species: Homo sapiens
Genetic Insert: homeobox A9
Vector Backbone Description: Backbone Marker:Dr. Richard Mulligan; Backbone Size:12152; Vector Backbone:pINDUCER21 (ORF-EG); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24094326
Comments: NOTE: The map and depositor sequence are of the empty backbone ONLY. Addgene does not have sequence for the fully assembled plasmid. Sequence for much of the insert can be found in Addgene's sequencing results.
Proper citation: RRID:Addgene_51302 Copy
Species: Homo sapiens
Genetic Insert: v-ets avian erythroblastosis virus E26 oncogene homolog
Vector Backbone Description: Backbone Marker:Dr. Richard Mulligan; Backbone Size:12152; Vector Backbone:pINDUCER21 (ORF-EG); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24094326
Comments: NOTE: The map and depositor sequence are of the empty backbone ONLY. Addgene does not have sequence for the fully assembled plasmid. Sequence for much of the insert can be found in Addgene's sequencing results.
Proper citation: RRID:Addgene_51301 Copy
Genetic Insert: NLS-HA-B3
Vector Backbone Description: Backbone Marker:CLONTECH; Backbone Size:4814; Vector Backbone:pDIRECT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24413561
Proper citation: RRID:Addgene_51270 Copy
Species: Streptomyces virginiae
Genetic Insert: BarB
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51155 Copy
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