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Species: Arabidopsis thaliana
Genetic Insert: AtU6-26:sgRNA
Vector Backbone Description: Backbone Marker:Promega Corp.; Backbone Size:3000; Vector Backbone:pGEM-T-easy; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25327456
Comments: Based on pEn-Chimera; MluI and Bsu36I RS added to each side of the U6-26:sgRNA sequence for cloning of up to 3 sgRNAs into Cas9 expression vector.
Proper citation: RRID:Addgene_61479 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtU6-26:sgRNA
Vector Backbone Description: Backbone Marker:Promega Corp.; Backbone Size:3000; Vector Backbone:pGEM-T-easy; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24836556
Proper citation: RRID:Addgene_61432 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:7100; Vector Backbone:pPZP221; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:24836556
Proper citation: RRID:Addgene_61433 Copy
Species: Arabidopsis thaliana
Genetic Insert: Promoter of GC1(D1)
Vector Backbone Description: Vector Backbone:pBI101; Vector Types:Plant expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18284694
Proper citation: RRID:Addgene_49513 Copy
Species: Arabidopsis thaliana
Genetic Insert: UVR8-GFPCaax
Vector Backbone Description: Vector Backbone:pEGFP N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23671313
Proper citation: RRID:Addgene_49805 Copy
Species: Arabidopsis thaliana
Genetic Insert: UVR8-mCherry
Vector Backbone Description: Vector Backbone:mCherry N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23671313
Proper citation: RRID:Addgene_49804 Copy
Species: Arabidopsis thaliana
Genetic Insert: 5' UTR, RbcS2B (AT5g38420, A. thaliana)
Vector Backbone Description: Vector Backbone:pICH41246; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50290 Copy
Species: Arabidopsis thaliana
Genetic Insert: promoter, act2 (AT3G18780, Arabidopsis thaliana)
Vector Backbone Description: Vector Backbone:pICH41233; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50256 Copy
Species: Arabidopsis thaliana
Genetic Insert: promoter, RbcS3B (AT5g38410, A. thaliana)
Vector Backbone Description: Vector Backbone:pICH41233; Vector Types:Other, plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50260 Copy
Species: Arabidopsis thaliana
Genetic Insert: promoter + 5' UTR, RbcS2B (AT5g38420, A. thaliana)
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50275 Copy
Species: Arabidopsis thaliana
Genetic Insert: promoter + 5' UTR, LHB1B1 (AT2g34430, A. thaliana)
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50278 Copy
Species: Arabidopsis thaliana
Genetic Insert: 3'UTR, polyadenylation signal/terminator, act2 (A. thaliana)
Vector Backbone Description: Vector Backbone:pICH41276; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50340 Copy
Species: Arabidopsis thaliana
Genetic Insert: PhyB 1-908
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909100
Comments: Please note this plasmid is a crucial component of the system described in this additional reference: Toettcher JE, Weiner OD, Lim WA, Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004. (PMID 24315106)
Proper citation: RRID:Addgene_50839 Copy
Species: Arabidopsis thaliana
Genetic Insert: WUS promoter
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
WUS promoter = 2252-6681bp
BsaI site #2 = 6682-6692bp
Proper citation: RRID:Addgene_48814 Copy
Species: Arabidopsis thaliana
Genetic Insert: AP3 promoter
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
AP3 promoter = 2252-3526bp
BsaI site #2 = 3527-3537bp
Proper citation: RRID:Addgene_48813 Copy
Species: Arabidopsis thaliana
Genetic Insert: At1g04880 terminator
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
At1g04480 terminator = 2252-2758bp
BsaI site #2 = 2759-2769bp
Proper citation: RRID:Addgene_48854 Copy
Species: Arabidopsis thaliana
Genetic Insert: UBQ10 terminator
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
UBQ10 terminator = 2252-2879bp
BsaI site #2 = 2880-2890bp
Proper citation: RRID:Addgene_48841 Copy
Species: Arabidopsis thaliana
Genetic Insert: linker:nuclear localization signal
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
linker-nuclear localization signal = 2252-2550bp
BsaI site #2 = 2551-2561bp
Proper citation: RRID:Addgene_48837 Copy
Species: Arabidopsis thaliana
Genetic Insert: WUS
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
WUS coding sequence = 2252-3129bp
BsaI site #2 = 3130-3140bp
Proper citation: RRID:Addgene_48824 Copy
Species: Arabidopsis thaliana
Genetic Insert: GAI
Vector Backbone Description: Vector Backbone:Lyn-CFP-FRB; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22446836
Comments: Lyn-CFP-FRB was created by inserting Lyn into the pEGFP- C1 vector (Clontech) whose EGFP is replaced with CFP-FRB.
Proper citation: RRID:Addgene_37313 Copy
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