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Species: Mus musculus
Genetic Insert: Gli2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pEF5-FRT-DEST; Vector Types:Mammalian Expression, Flpin system; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24373970
Proper citation: RRID:Addgene_51252 Copy
Species: Mus musculus
Genetic Insert: Gli2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pEF5-FRT-DEST; Vector Types:Mammalian Expression, Flpin system; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24373970
Proper citation: RRID:Addgene_51251 Copy
Genetic Insert: Co-InCreN
Vector Backbone Description: Backbone Marker:CLONTECH; Backbone Size:4814; Vector Backbone:pDIRECT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24413561
Comments: Please note that the complementary plasmid pCAG-Co-InCreC (www.addgene.org/51268) must be ordered with pCAG-Co-InCreN in order to successfully use the Co-Driver system as described in the associated publication.
Proper citation: RRID:Addgene_51267 Copy
Genetic Insert: b3STOPb3-ZsGreen
Vector Backbone Description: Backbone Marker:CLONTECH; Backbone Size:4814; Vector Backbone:pDIRECT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24413561
Proper citation: RRID:Addgene_51266 Copy
Species: Synthetic
Genetic Insert: 3xFLAG-dCas9
Vector Backbone Description: Backbone Marker:Hodaka Fujii; Backbone Size:6238; Vector Backbone:pMXs-neo; Vector Types:Mammalian Expression, Retroviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25051498
Comments: The coding sequence of 3xFLAG-dCas9 can be cleaved with Pac I and Not I.
Construction strategy of gRNA retroviral vectors
1. Cleave gBlock from a gRNA vector constructed using gRNA cloning vector (Addgene #41824) with appropriate restriction enzymes (eg. [Xho I + Hind III], EcoR I).
2. Insert the cleaved gBlock into pSIR-based self-inactivating retroviral vectors.
Vectors & Sites of insertion
pSIR-neo (Addgene #51128): eg. [Xho I + Hind III]
pSIR-GFP (Addgene #51134): eg. [Xho I + Hind III], EcoR I
pSIR-DsRed-Express2 (Addgene #51135): eg. [Xho I + Hind III], EcoR I
pSIR-hCD2 (Addgene #51143): eg. EcoR I
Proper citation: RRID:Addgene_51260 Copy
Species: Synthetic
Genetic Insert: Pyronic
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5419; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24465702
Proper citation: RRID:Addgene_51308 Copy
Species: Homo sapiens
Genetic Insert: homeobox A9
Vector Backbone Description: Backbone Marker:Dr. Richard Mulligan; Backbone Size:12152; Vector Backbone:pINDUCER21 (ORF-EG); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24094326
Comments: NOTE: The map and depositor sequence are of the empty backbone ONLY. Addgene does not have sequence for the fully assembled plasmid. Sequence for much of the insert can be found in Addgene's sequencing results.
Proper citation: RRID:Addgene_51302 Copy
Species: Homo sapiens
Genetic Insert: v-ets avian erythroblastosis virus E26 oncogene homolog
Vector Backbone Description: Backbone Marker:Dr. Richard Mulligan; Backbone Size:12152; Vector Backbone:pINDUCER21 (ORF-EG); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24094326
Comments: NOTE: The map and depositor sequence are of the empty backbone ONLY. Addgene does not have sequence for the fully assembled plasmid. Sequence for much of the insert can be found in Addgene's sequencing results.
Proper citation: RRID:Addgene_51301 Copy
Genetic Insert: NLS-HA-B3
Vector Backbone Description: Backbone Marker:CLONTECH; Backbone Size:4814; Vector Backbone:pDIRECT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24413561
Proper citation: RRID:Addgene_51270 Copy
Species: Streptomyces virginiae
Genetic Insert: BarB
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51155 Copy
Genetic Insert: Roxed-Cre
Vector Backbone Description: Vector Backbone:pTS(2); Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24413561
Proper citation: RRID:Addgene_51276 Copy
Species: Bacillus megaterium
Genetic Insert: BM1P1
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51157 Copy
Vector Backbone Description: Backbone Marker:CLONTECH; Backbone Size:4814; Vector Backbone:pDIRECT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24413561
Proper citation: RRID:Addgene_51278 Copy
Species: Escherichia coli
Genetic Insert: BetI
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51156 Copy
Genetic Insert: NLS-HA-Dre-P2A-BFP
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24413561
Proper citation: RRID:Addgene_51277 Copy
Genetic Insert: Dre
Vector Backbone Description: Backbone Marker:CLONTECH; Backbone Size:4814; Vector Backbone:pDIRECT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24413561
Comments: Please note that the complementary plasmid pCAG-Roxed-Cre (www.addgene.org/51273) must be ordered with pCAG-NLS-HA-Dre in order to successfully use the Co-Driver recombinase system as described in the associated publication.
Proper citation: RRID:Addgene_51272 Copy
Species: Pseudomonas aeruginosa
Genetic Insert: AmrR
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51150 Copy
Genetic Insert: E6LLO
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pMal; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23832121
Proper citation: RRID:Addgene_51281 Copy
Species: Bacillus megaterium
Genetic Insert: Cyp106
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51166 Copy
Species: Homo sapiens
Genetic Insert: U6
Vector Backbone Description: Backbone Marker:Addgene plasmid 51283; Vector Backbone:pAluYa5-neo-TET; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19390602
Comments: U6 is tagged with the self splicing intron neo cassette such that G418 resistance will be obtained only if retrotransposition occurs.
Please note that the cassette is in the reverse orientation within the MCS relative to the full sequence--This has no functional consequence.
Proper citation: RRID:Addgene_51286 Copy
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