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Species: Synthetic
Genetic Insert: entry vector for FnCas12a crRNAs
Vector Backbone Description: Backbone Marker:Joung Lab (Addgene plasmid # 43860); Vector Backbone:MLM3636; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30742127
Proper citation: RRID:Addgene_114087 Copy
Species: Homo sapiens
Genetic Insert: REV7
Vector Backbone Description: Backbone Size:5864; Vector Backbone:pDEST-FRT/TO-eGFP; Vector Types:Mammalian Expression, Genomic integration, tetracycline inducible; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30022168
Proper citation: RRID:Addgene_114128 Copy
Species: Synthetic
Genetic Insert: human codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag
Vector Backbone Description: Backbone Marker:Schier Lab (Addgene plasmid # 47327); Vector Backbone:pET-28b-Cas9-His; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30742127
Proper citation: RRID:Addgene_114075 Copy
Genetic Insert: sbGLuc-VChR1-EYFP
Vector Backbone Description: Backbone Size:4552; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35126293
Proper citation: RRID:Addgene_114103 Copy
Genetic Insert: Gaussia luciferase, slow burn; CheRiff synthetic ChR; EGFP
Vector Backbone Description: Backbone Size:4269; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36361784
Proper citation: RRID:Addgene_114108 Copy
Species: Synthetic
Genetic Insert: dCas9-VPR
Vector Backbone Description: Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30863790
Comments: Addgene #63798 into lentivirus backbone and addition of N-term FLAG tag and SYN promoter . Please visit https://www.biorxiv.org/content/early/2018/07/17/371500 for bioRxiv preprint.
Proper citation: RRID:Addgene_114196 Copy
Species: Homo sapiens
Genetic Insert: Amyloid-Precursor-Protein
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pcDNA FRT TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_114194 Copy
Genetic Insert: sgRNA scaffold
Vector Backbone Description: Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30863790
Comments: Addgene #47108 adapted into lentivirus backbone and insertion of EF1a mCherry . Please visit https://www.biorxiv.org/content/early/2018/07/17/371500 for bioRxiv preprint.
Proper citation: RRID:Addgene_114199 Copy
Species: Homo sapiens
Genetic Insert: Muscarin acetylcholine receptor 3
Vector Backbone Description: Backbone Size:4727; Vector Backbone:pPA-mCherry N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_114189 Copy
Species: Homo sapiens
Genetic Insert: Amyloid-Precursor-Protein
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pcDNA FRT TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_114193 Copy
Species: Synthetic
Genetic Insert: tagRFP-KDEL
Vector Backbone Description: Backbone Marker:Invitrogen/Thermo; Backbone Size:2717; Vector Backbone:pENTR1a; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29176619
Proper citation: RRID:Addgene_114177 Copy
Species: Homo sapiens
Genetic Insert: STIM1
Vector Backbone Description: Backbone Marker:Invitrogen/Thermo; Backbone Size:2717; Vector Backbone:pENTR1a; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29176619
Proper citation: RRID:Addgene_114176 Copy
Species: Flavobacterium meningosepticum
Genetic Insert: Peptide: N-glycosidase F
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS(-); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_114274 Copy
Species: Mus musculus
Genetic Insert: Itch
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5400; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12907674
Comments: Plasmid contains ~1kb of 3' UTR of the mouse Itch gene.
Proper citation: RRID:Addgene_11427 Copy
Species: Mus musculus
Genetic Insert: Nedd4-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12907674
Proper citation: RRID:Addgene_11426 Copy
Species: Synthetic
Genetic Insert: 3xNLS_SpCas9
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-21 a; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30911135
Comments: This plasmid is also associated with the following publication:
PMID 30911135: Highly efficient therapeutic gene editing of human hematopoietic stem cells
Proper citation: RRID:Addgene_114365 Copy
Species: Homo sapiens
Genetic Insert: Kir2.1
Vector Backbone Description: Vector Backbone:pCWXPGR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
Proper citation: RRID:Addgene_114284 Copy
Species: Homo sapiens
Genetic Insert: rERK2 and hMEK1 proteins
Vector Backbone Description: Vector Backbone:pCWXPGR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: rat ERK2 fused to human MEK1 with a linker in between. L4A mutations are in the human MEK1 NES. Subcloned from Addgene plasmid #39197. pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
Proper citation: RRID:Addgene_114291 Copy
Species: Homo sapiens
Genetic Insert: Ras
Vector Backbone Description: Vector Backbone:pCLXEBR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Due to the role of the transgene in cell proliferation, lentivectors produced with human-tropic envelopes must be produced in BSL2+/BSL3 conditions. Subcloned from Addgene Plasmid #31200. pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
Proper citation: RRID:Addgene_114318 Copy
Species: Homo sapiens
Genetic Insert: hTERT
Vector Backbone Description: Vector Backbone:pCWX-PGK-BSD; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23097121
Comments: Due to the role of the transgene in cell proliferation, lentivectors produced with human-tropic envelopes must be produced in BSL2+/BSL3 conditions.
Proper citation: RRID:Addgene_114316 Copy
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