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Species: Synthetic
Genetic Insert: human codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag
Vector Backbone Description: Backbone Marker:Schier Lab (Addgene plasmid # 47327); Vector Backbone:pET-28b-Cas9-His; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30742127
Proper citation: RRID:Addgene_114075 Copy
Species: Synthetic
Genetic Insert: human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.1)
Vector Backbone Description: Backbone Marker:Cepko Lab (Addgene plasmid #11179); Vector Backbone:pCAG-CFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30742127
Proper citation: RRID:Addgene_114079 Copy
Species: Synthetic
Genetic Insert: DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.3)
Vector Backbone Description: Backbone Marker:Cepko Lab (Addgene plasmid #11179); Vector Backbone:pCAG-CFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30742127
Proper citation: RRID:Addgene_114083 Copy
Species: Synthetic
Genetic Insert: AKAR5' C-terminally tagged by KRas C tail
Vector Backbone Description: Backbone Size:5900; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28423323
Proper citation: RRID:Addgene_114140 Copy
Species: Synthetic
Genetic Insert: LacZalpha
Vector Backbone Description: Vector Backbone:pMXBC; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29986052
Proper citation: RRID:Addgene_114252 Copy
Species: Synthetic
Genetic Insert: LacZalpha
Vector Backbone Description: Vector Backbone:pMXBC; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29986052
Proper citation: RRID:Addgene_114251 Copy
Species: Synthetic
Genetic Insert: LacZalpha
Vector Backbone Description: Vector Backbone:pMXBK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29986052
Proper citation: RRID:Addgene_114255 Copy
Species: Synthetic
Genetic Insert: LacZalpha
Vector Backbone Description: Vector Backbone:pMXBK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29986052
Proper citation: RRID:Addgene_114259 Copy
Species: Synthetic
Genetic Insert: LacZalpha
Vector Backbone Description: Vector Backbone:pMXBK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29986052
Proper citation: RRID:Addgene_114258 Copy
Species: Synthetic
Genetic Insert: LacZalpha
Vector Backbone Description: Vector Backbone:pMXBK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29986052
Proper citation: RRID:Addgene_114257 Copy
Species: Synthetic
Genetic Insert: LacZalpha
Vector Backbone Description: Vector Backbone:pMXLP; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29986052
Proper citation: RRID:Addgene_114241 Copy
Species: Synthetic
Genetic Insert: LacZalpha
Vector Backbone Description: Vector Backbone:pMXLP; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29986052
Proper citation: RRID:Addgene_114240 Copy
Species: Synthetic
Genetic Insert: LacZalpha
Vector Backbone Description: Vector Backbone:pMXLP; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29986052
Proper citation: RRID:Addgene_114242 Copy
Species: Synthetic
Genetic Insert: dCas9-VPR
Vector Backbone Description: Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30863790
Comments: Addgene #63798 into lentivirus backbone and addition of N-term FLAG tag and SYN promoter . Please visit https://www.biorxiv.org/content/early/2018/07/17/371500 for bioRxiv preprint.
Proper citation: RRID:Addgene_114196 Copy
Species: Synthetic
Genetic Insert: LacZalpha
Vector Backbone Description: Vector Backbone:pMXLK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29986052
Proper citation: RRID:Addgene_114233 Copy
Species: Synthetic
Genetic Insert: LacZalpha
Vector Backbone Description: Vector Backbone:pMXLK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29986052
Proper citation: RRID:Addgene_114232 Copy
Species: Synthetic
Genetic Insert: LacZalpha
Vector Backbone Description: Vector Backbone:pMXLK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29986052
Proper citation: RRID:Addgene_114238 Copy
Species: Synthetic
Genetic Insert: LacZalpha
Vector Backbone Description: Vector Backbone:pMXLC; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29986052
Proper citation: RRID:Addgene_114222 Copy
Species: Synthetic
Genetic Insert: LacZalpha
Vector Backbone Description: Vector Backbone:pMXLC; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29986052
Proper citation: RRID:Addgene_114225 Copy
Species: Synthetic
Genetic Insert: LacZalpha
Vector Backbone Description: Vector Backbone:pMXLC; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29986052
Proper citation: RRID:Addgene_114228 Copy
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