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Species: Caenorhabditis elegans
Genetic Insert: sqt-1(e1350) gRNA2
Vector Backbone Description: Vector Backbone:pRB1017; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25161212
Proper citation: RRID:Addgene_59935 Copy
Species: Caenorhabditis elegans
Genetic Insert: rab-3 promoter
Vector Backbone Description: Vector Backbone:pCFJ150; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25377555
Proper citation: RRID:Addgene_59788 Copy
Species: Caenorhabditis elegans
Genetic Insert: rab-3 promoter
Vector Backbone Description: Vector Backbone:pCFJ150; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25377555
Proper citation: RRID:Addgene_59787 Copy
Species: Caenorhabditis elegans
Genetic Insert: ZF1 domain from pie-1
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pBR322; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25377555
Proper citation: RRID:Addgene_59790 Copy
Species: Caenorhabditis elegans
Genetic Insert: rde-1(D718) gRNA
Vector Backbone Description: Vector Backbone:pRB1017; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25161212
Proper citation: RRID:Addgene_59927 Copy
Species: Caenorhabditis elegans
Genetic Insert: dhc-3
Vector Backbone Description: Backbone Marker:Andrew Fire; Backbone Size:2790; Vector Backbone:L4440; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25066299
Comments: Source BioScience LifeSciences C. elegans RNAi library clones utilized in plasmid construction (Fraser, A.G., Kamath, R.S., Zipperlen, P., Martinez-Campos, M., Sohrmann, M. and Ahringer, J. (2000) Functional genomic analysis of C. elegans chromosome I by systematic RNA interference. Nature 408, 325–330.)
Proper citation: RRID:Addgene_59997 Copy
Species: Caenorhabditis elegans
Genetic Insert: dhc-3
Vector Backbone Description: Backbone Marker:Andrew Fire; Backbone Size:2790; Vector Backbone:L4440; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25066299
Comments: Source BioScience LifeSciences C. elegans RNAi library clones utilized in plasmid construction (Fraser, A.G., Kamath, R.S., Zipperlen, P., Martinez-Campos, M., Sohrmann, M. and Ahringer, J. (2000) Functional genomic analysis of C. elegans chromosome I by systematic RNA interference. Nature 408, 325–330.)
Proper citation: RRID:Addgene_59996 Copy
Species: Caenorhabditis elegans
Genetic Insert: sqt-1(e1350) gRNA1
Vector Backbone Description: Vector Backbone:pRB1017; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25161212
Proper citation: RRID:Addgene_60212 Copy
Species: Caenorhabditis elegans
Genetic Insert: U6 promoter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3519; Vector Backbone:pCR-Blunt II-TOPO; Vector Types:CRISPR, Cloning template; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25491644
Proper citation: RRID:Addgene_61253 Copy
Species: Caenorhabditis elegans
Genetic Insert: PPTR-1/pLexA-N bait
Vector Backbone Description: Backbone Size:5650; Vector Backbone:pLexA-N Gateway compatible destination vector; Vector Types:Yeast Expression, two-hybrid bait vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62866 Copy
Species: Caenorhabditis elegans
Genetic Insert: MBP-PPTR-1
Vector Backbone Description: Backbone Size:8356; Vector Backbone:pMAL-c2X Gateway compatible destination vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62868 Copy
Species: Caenorhabditis elegans
Genetic Insert: MBP-MEG-1 S574A
Vector Backbone Description: Backbone Size:8356; Vector Backbone:pMAL-c2X Gateway compatible destination vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62877 Copy
Species: Caenorhabditis elegans
Genetic Insert: MBP-MEG-3 T541A S582A T605A
Vector Backbone Description: Backbone Size:8356; Vector Backbone:pMAL-c2X Gateway compatible destination vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62878 Copy
Species: Caenorhabditis elegans
Genetic Insert: MBP-MEI-1
Vector Backbone Description: Backbone Size:8356; Vector Backbone:pMAL-c2X Gateway compatible destination vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62873 Copy
Species: Caenorhabditis elegans
Genetic Insert: MBP-PAA-1
Vector Backbone Description: Backbone Size:8356; Vector Backbone:pMAL-c2X Gateway compatible destination vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62870 Copy
Species: Caenorhabditis elegans
Genetic Insert: MBP-MBK-2
Vector Backbone Description: Backbone Size:8356; Vector Backbone:pMAL-c2X Gateway compatible destination vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62871 Copy
Species: Caenorhabditis elegans
Genetic Insert: GST-MEG-3
Vector Backbone Description: Backbone Size:4984; Vector Backbone:pGEX6p1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62880 Copy
Species: Caenorhabditis elegans
Genetic Insert: C. elegans UNC-60B cDNA
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:459; Vector Backbone:pET-3d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9452511
Comments: Please see the insert sequence provided by the depositing laboratory and Figure 1 from the associated publication (Ono S and Benian GM, JBC 1998) listed below for information on the UNC-60 isoform in this plasmid. This plasmid encodes the UNC-60B isoform as described in the publication and now referred to as UNC-60C by NCBI and Wormbase (GenBank reference sequence NP_503427.2).
The original nomenclature of UNC-60A and UNC-60B was defined in the publication "Genetic organization of the unc-60 region in Caenorhabditis elegans." McKim KS, Heschl MF, Rosenbluth RE, Baillie DL. Genetics. 1988 Jan;118(1):49-59. https://www.ncbi.nlm.nih.gov/pubmed/8608931
Proper citation: RRID:Addgene_48740 Copy
Species: Caenorhabditis elegans
Genetic Insert: snf-5
Vector Backbone Description: Backbone Size:5052; Vector Backbone:pXOOM; Vector Types:Mammalian Expression, Xenopus Oocyte Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23580723
Proper citation: RRID:Addgene_48697 Copy
Species: Caenorhabditis elegans
Genetic Insert: GLH-1::nos-2 3’UTR
Vector Backbone Description: Backbone Marker:custom; Backbone Size:6700; Vector Backbone:pEV1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20335358
Proper citation: RRID:Addgene_37487 Copy
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