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Species: Synthetic
Genetic Insert: GFP+
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:5796; Vector Backbone:pTU1-A - EcoFlex (Moore et al, 2016 - PubMed PMID: 27096716); Vector Types:Bacterial Expression, Synthetic Biology, E. coli and Bacillus shuttle vector; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:29666238
Proper citation: RRID:Addgene_107582 Copy
Species: Synthetic
Genetic Insert: GFP+
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:6336; Vector Backbone:pTU1-A - EcoFlex (Moore et al, 2016 - PubMed PMID: 27096716); Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:29666238
Proper citation: RRID:Addgene_107578 Copy
Species: Homo sapiens
Genetic Insert: Bystin
Vector Backbone Description: Backbone Size:4033; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:9560222
Proper citation: RRID:Addgene_11869 Copy
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Diatom cloning; Bacterial Resistance:Ampicillin and Tetracycline
Comments: selectable marker added for centric diatoms
Proper citation: RRID:Addgene_154035 Copy
Species: Synthetic
Genetic Insert: A/PuertoRico/8/34 hemagglutinin
Vector Backbone Description: Backbone Marker:-; Backbone Size:3500; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:30597163
Proper citation: RRID:Addgene_157933 Copy
Species: Human
Genetic Insert: Neuraminidase A/NL/354/16 H3N2
Vector Backbone Description: Backbone Marker:-; Backbone Size:4800; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:32710576
Proper citation: RRID:Addgene_158742 Copy
Genetic Insert: Hemagglutinin A/MI/45/15 H1N1
Vector Backbone Description: Backbone Marker:-; Backbone Size:5400; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:32710576
Proper citation: RRID:Addgene_158740 Copy
Species: Homo sapiens
Genetic Insert: integrin alpha 9 / alpha 5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:11598204
Comments: See article for cloning details.
Proper citation: RRID:Addgene_13588 Copy
Species: Homo sapiens
Genetic Insert: integrin alpha 9
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:7523411
Comments: Please note that the first 41 amino acids of the integrin alpha insert show greater identity to integrin alpha-5 rather than integrin alpha-9
Proper citation: RRID:Addgene_13585 Copy
Species: Homo sapiens
Genetic Insert: integrin beta 6 777T
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:12634787
Proper citation: RRID:Addgene_13582 Copy
Species: Synthetic
Genetic Insert: T7A1, O-, O1, Lambda t1 in lacZ fragment
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Comments: LacI operators separated by 400 base pairs.
Proper citation: RRID:Addgene_170473 Copy
Species: Synthetic
Genetic Insert: T7A1, Os, O2, Lambda t1 in lacZ fragment
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Comments: LacI operators separated by 400 base pairs.
Proper citation: RRID:Addgene_170471 Copy
Species: Synthetic
Genetic Insert: T7A1, O2, O1, Lambda t1 in lacZ fragment
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:28295806
Comments: LacI operators separated by 400 base pairs.
Proper citation: RRID:Addgene_170477 Copy
Species: Synthetic
Genetic Insert: T7A1, O2, Os, Lambda t1 in lacZ fragment
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Comments: LacI operators separated by 400 base pairs.
Proper citation: RRID:Addgene_170478 Copy
Species: Mus musculus
Genetic Insert: Gq alpha
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:8387644
Comments: This is Gq alpha with an HA epitope engineered into an internal site that does not seem to affect receptor coupling in multiple studies. Epitope tagged by Paul Wilson, see Wedegaertner, JBC 268: 25001-25008. The 5' non-coding sequences were removed, but the 3' non-coding sequences remain, as in Strathmann & Simon (1990) PNAS 87:9113-9117. The parent construct has been donated to the ATCC by the Bourne Lab.
There is a 1bp insertion, a 5bp insertion, and a 1bp deletion when author sequence is aligned with Addgene quality control sequence. These differences are between the stop codon and the SP6 sequence.
Please see supplemental document for G-protein chimera user manual.
Proper citation: RRID:Addgene_24502 Copy
Species: Synthetic
Genetic Insert: D/bovine/Nebraska/9-5/2012
Vector Backbone Description: Backbone Marker:-; Backbone Size:3500; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:33807137
Proper citation: RRID:Addgene_175020 Copy
Species: Synthetic
Genetic Insert: D/bovine/Nebraska/9-5/2012
Vector Backbone Description: Backbone Marker:-; Backbone Size:3500; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:33807137
Proper citation: RRID:Addgene_175019 Copy
Species: Caenorhabditis elegans
Genetic Insert: let-363 cDNA
Vector Backbone Description: Backbone Size:2790; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:22278922
Comments: This plasmid can be kept in DH5alpha cells but does not work for RNAi in that strain. For RNAi, please transform DNA into the HT115 strain.
Proper citation: RRID:Addgene_42600 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-15
Vector Backbone Description: Backbone Size:2790; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:22278922
Comments: This plasmid can be kept in DH5alpha cells but does not work for RNAi in that strain. For RNAi, please transform DNA into the HT115 strain.
Note: Addgene's quality control sequencing has found that the deposited clone contains a T to C mismatch with respect to the C. elegans N2 reference daf-15 sequence, which would cause a V797A change in the coded protein. According to the depositing lab, this point mutation does not affect RNAi feeding; however, its possible effects in other contexts are not known.
Proper citation: RRID:Addgene_42601 Copy
Species: Mus musculus
Genetic Insert: GlyCAM-1/IgG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4356; Vector Backbone:pCDM8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:10330415
Proper citation: RRID:Addgene_17841 Copy
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