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  • Bacterial Resistance:apramycin (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pMSR:MSMEG_5487:Dendra
 
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RRID:Addgene_167440 MSMEG_5487 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-15 01:09:44 0
pMSR:MSMEG_5471:Dendra
 
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RRID:Addgene_167434 MSMEG_5471 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-15 01:09:44 0
pMSR:MSMEG_5472:Dendra
 
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RRID:Addgene_167435 MSMEG_5472 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-15 01:09:44 0
pIB1884-ximD-PCPT
 
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RRID:Addgene_169109 XimD and PcPT Synthetic Apramycin PMID:32786357 Vector Backbone:pSE152; Vector Types:Synthetic Biology; Bacterial Resistance:Apramycin 2026-08-15 01:09:57 0
pYL-kasOp-FnCas12a3
 
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RRID:Addgene_177875 Streptomyces codon optimized the FnCas12a mutant EP16 Streptomyces Apramycin PMID:32695773 Vector Backbone:pCRISPomyces-2; Vector Types:CRISPR; Bacterial Resistance:Apramycin 2026-08-15 01:11:12 0
pSelAct-KO
 
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RRID:Addgene_174374 Apramycin PMID:34890250 Also known as pSelAct. For cloning in homology arms, can use Gateway, restriction-ligation, or homology-based cloning such as Gibson/Infusion cloning. Please cite at minimum paper 1) and a secondary paper that is most appropriate to your system of interest (2 or 3). 1. First development of pSelAct for knockouts in Rhodococcus equi: Geize, R. van der, Jong, W. de, Hessels, G. I., Grommen, A. W. F., Jacobs, A. A. C., and Dijkhuizen, L. 2008. A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality. Nucleic Acids Res. 36:e151 2. First development of pSelAct with Gateway cassette for knockouts in Rhodococcus fasicans: Savory, E. A., Weisberg, A. J., Stevens, D. M., Creason, A. L., Fuller, S. L., Pearce, E. M., and Chang, J. H. 2020. Phytopathogenic Rhodococcus Have Diverse Plasmids With Few Conserved Virulence Functions. Front Microbiol. 11:1022 3. Later referred to as pSelAct-KO after optimization for knockouts in Clavibacter:  Stevens, D. M., Tang, A., and Coaker, G. 2021. A Genetic Toolkit for Investigating Clavibacter Species: Markerless Deletion, Permissive Site Identification, and an Integrative Plasmid. Mol Plant-microbe Interactions. 34:1336–1345 Backbone Size:7373; Vector Backbone:pUC19; Vector Types:Vector for 2-step knockout by homologous recombination; Bacterial Resistance:Apramycin 2026-08-15 01:10:39 0
pBAD_Ub11TCA
 
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RRID:Addgene_174517 Ubiquitin Homo sapiens Apramycin PMID:34083482 Backbone Size:3896; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin Recoded with TCA replacing codon 11 of wt ubiquitin 2026-08-15 01:10:41 0
pMSR:MSMEG_0948:Dendra
 
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RRID:Addgene_174952 MSMEG_0948 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-15 01:10:45 0
pMSR:MSMEG_0952:Dendra
 
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RRID:Addgene_174954 MSMEG_0952 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-15 01:10:45 0
pMSR:MSMEG_0953:Dendra
 
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RRID:Addgene_174955 MSMEG_0953 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-15 01:10:45 0
pMSR:MSMEG_0954:Dendra
 
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RRID:Addgene_174956 MSMEG_0954 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-15 01:10:45 0
pMSR:MSMEG_0956:Dendra
 
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RRID:Addgene_174957 MSMEG_0956 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-15 01:10:45 0
pMSR:MSMEG_0970:Dendra
 
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RRID:Addgene_174959 MSMEG_0970 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-15 01:10:45 0
pMSR:MSMEG_0946:Dendra
 
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RRID:Addgene_174950 MSMEG_0946 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-15 01:10:45 0
pMSR:MSMEG_0943:Dendra
 
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RRID:Addgene_174949 MSMEG_0943 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-15 01:10:45 0
pMSR:MSMEG_0933:Dendra
 
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RRID:Addgene_174941 MSMEG_0933 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-15 01:10:45 0
pMSR:MSMEG_0934:Dendra
 
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RRID:Addgene_174942 MSMEG_0934 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-15 01:10:45 0
pMSR:MSMEG_0937:Dendra
 
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RRID:Addgene_174945 MSMEG_0937 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-15 01:10:45 0
pMSR:MSMEG_0938:Dendra
 
Resource Report
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RRID:Addgene_174946 MSMEG_0938 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-15 01:10:45 0
pMSR:MSMEG_0939:Dendra
 
Resource Report
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RRID:Addgene_174947 MSMEG_0939 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-15 01:10:45 0

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