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Plasmids are provided by Addgene and DGRC.

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pTEC27
 
Resource Report
Resource Website
10+ mentions 		RRID:Addgene_30182 Mycobacterium Strong Promoter (MSP) M. marinum Hygromycin PMID:23680983 This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with tdTomato. The tdTomato ORF is present at bp# 102-1532. Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin 2026-08-15 01:13:26 32
pGFPHYG2
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_30173 Mycobacterium Strong Promoter (MSP) M. marinum Hygromycin PMID:16714540 This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin GFPmut3 allele has improved brightness. 2026-08-15 01:13:25 5
pTEC16
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_30175 Mycobacterium Strong Promoter (MSP) M. marinum Hygromycin PMID:23680983 This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with mOrange2. The mOrange2 ORF is present at bp# 5620-6330. Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin 2026-08-15 01:13:25 3
pTEC20
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_30179 Mycobacterium Strong Promoter (MSP) M. marinum Hygromycin PMID:23680983 This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with mKate2. The mKate2 ORF is present at bp# 105-803. Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin 2026-08-15 01:13:26 3
pDB88
 
Resource Report
Resource Website 		RRID:Addgene_26958 e. coli galactokinase Escherichia coli Hygromycin PMID:20851171 Backbone Size:10000; Vector Backbone:PMSG346; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin cloned under the MOP promoter 2026-08-15 01:12:58 0
pGMEH-P38-luxAB2
 
Resource Report
Resource Website 		RRID:Addgene_27061 P#38-luxAB synthetic gene Hygromycin PMID:21075796 Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-08-15 01:12:59 0
pGMEH-P38-che-DAS+4
 
Resource Report
Resource Website 		RRID:Addgene_27060 P#38-mcherry rfp-DAS+4 synthetic gene Hygromycin PMID:21075796 Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-08-15 01:12:59 0
pGMEH-P38-mRFP
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_27058 P#38-mcherry rfp synthetic gene Hygromycin PMID:21075796 Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-08-15 01:12:59 4
pTE-0X38-GCT6
 
Resource Report
Resource Website 		RRID:Addgene_27057 P#38-gfp-DAS+4 synthetic gene Hygromycin PMID:21075796 pTE-mcs derivative (Addgene plasmid# 20320) with GFP-DAS+4 under the control of a strong promoter. DAS+4 sequence is AANDENYSENYADAS at end of GFP. Backbone Size:6087; Vector Backbone:pTE-0X1-GCT6; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-08-15 01:12:59 0
pGMEH-P38-luxAFB-DAS+4
 
Resource Report
Resource Website 		RRID:Addgene_27064 P#38-luxA-FLAG-luxB-DAS+4 synthetic gene Hygromycin PMID:21075796 Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-08-15 01:12:59 0
pMSG360
 
Resource Report
Resource Website 		RRID:Addgene_27155 Hygromycin PMID:20472794 Note that Addgene's sequencing results differ from the depositor's sequence at bp# 561, 565 and 829. Depositing scientist states that these differences do not affect the function of the plasmid. Backbone Marker:Glickman Lab; Backbone Size:4152; Vector Backbone:pmsg360; Vector Types:mycobacterial ko vector; Bacterial Resistance:Hygromycin 2026-08-15 01:13:00 0
pGMEH-10M1-clpX-K127R
 
Resource Report
Resource Website 		RRID:Addgene_27374 tetR(sB), clpXtb-K127R Hygromycin PMID:19174563 Backbone Marker:N/A; Backbone Size:6181; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Hygromycin changed Lysine 127 to Arginine 2026-08-15 01:13:02 0
pTE-mcs
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_20320 multiple cloning site Hygromycin PMID:19174563 Backbone Size:5059; Vector Backbone:pMS2; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-08-15 01:11:53 1
pTE-10M0X
 
Resource Report
Resource Website 		RRID:Addgene_20318 Pimyc-tetR#10 synthetic gene derived from Tn10 tetR Hygromycin PMID:19174563 There are minor discrepancies between depositor's and Addgene's sequence. Depositor is aware of these and thinks they should not have any affect on function. Backbone Size:5133; Vector Backbone:pTE-mcs; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-08-15 01:11:53 0
pCHERRY8
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_24663 mCherry Synthetic gene Hygromycin PMID:20352111 Backbone Size:5582; Vector Backbone:pSMT3-rpsA; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-08-15 01:12:33 3
pCHERRY10
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_24664 mCherry Synthetic gene Hygromycin PMID:20352111 Backbone Size:5582; Vector Backbone:pSMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-08-15 01:12:33 3
pKW08-Lx-Int
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_25015 pKW08-Lx-Int Hygromycin PMID:20434484 Backbone Size:8535; Vector Backbone:pMC1M; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-08-15 01:12:36 2
pJDC122
 
Resource Report
Resource Website 		RRID:Addgene_157876 tdTomato Hygromycin PMID:26934495 Vector Backbone:pJDC89; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-08-15 01:08:09 0
pBSV2H_Psyn-sgRNAflaB
 
Resource Report
Resource Website 		RRID:Addgene_149561 sgRNAflaB Synthetic Hygromycin PMID:33257311 Addgene QC identified an IS element present in the backbone sequence. The depositing lab does not believe this impacts the functional use of the plasmid but requesting scientists should validate expected behavior prior to experimentation. Backbone Marker:CJW lab; Vector Backbone:pBSV2H; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin 2026-08-15 01:07:17 0
pBSV2H_Psyn-sgRNAmreB
 
Resource Report
Resource Website 		RRID:Addgene_149567 sgRNAmreB Synthetic Hygromycin PMID:33257311 Backbone Marker:CJW lab; Vector Backbone:pBSV2H; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin 2026-08-15 01:07:16 0

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