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Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with tdTomato. The tdTomato ORF is present at bp# 102-1532.
Proper citation: RRID:Addgene_30182 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:16714540
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance.
Proper citation: RRID:Addgene_30173 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with mOrange2. The mOrange2 ORF is present at bp# 5620-6330.
Proper citation: RRID:Addgene_30175 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with mKate2. The mKate2 ORF is present at bp# 105-803.
Proper citation: RRID:Addgene_30179 Copy
Species: Escherichia coli
Genetic Insert: e. coli galactokinase
Vector Backbone Description: Backbone Size:10000; Vector Backbone:PMSG346; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20851171
Proper citation: RRID:Addgene_26958 Copy
Species: synthetic gene
Genetic Insert: P#38-luxAB
Vector Backbone Description: Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Proper citation: RRID:Addgene_27061 Copy
Species: synthetic gene
Genetic Insert: P#38-mcherry rfp-DAS+4
Vector Backbone Description: Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Proper citation: RRID:Addgene_27060 Copy
Species: synthetic gene
Genetic Insert: P#38-mcherry rfp
Vector Backbone Description: Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Proper citation: RRID:Addgene_27058 Copy
Species: synthetic gene
Genetic Insert: P#38-gfp-DAS+4
Vector Backbone Description: Backbone Size:6087; Vector Backbone:pTE-0X1-GCT6; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Comments: pTE-mcs derivative (Addgene plasmid# 20320) with GFP-DAS+4 under the control of a strong promoter. DAS+4 sequence is AANDENYSENYADAS at end of GFP.
Proper citation: RRID:Addgene_27057 Copy
Species: synthetic gene
Genetic Insert: P#38-luxA-FLAG-luxB-DAS+4
Vector Backbone Description: Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Proper citation: RRID:Addgene_27064 Copy
Vector Backbone Description: Backbone Marker:Glickman Lab; Backbone Size:4152; Vector Backbone:pmsg360; Vector Types:mycobacterial ko vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20472794
Comments: Note that Addgene's sequencing results differ from the depositor's sequence at bp# 561, 565 and 829. Depositing scientist states that these differences do not affect the function of the plasmid.
Proper citation: RRID:Addgene_27155 Copy
Genetic Insert: tetR(sB), clpXtb-K127R
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:6181; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Hygromycin
Defining Citation: PMID:19174563
Proper citation: RRID:Addgene_27374 Copy
Genetic Insert: multiple cloning site
Vector Backbone Description: Backbone Size:5059; Vector Backbone:pMS2; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:19174563
Proper citation: RRID:Addgene_20320 Copy
Species: synthetic gene derived from Tn10 tetR
Genetic Insert: Pimyc-tetR#10
Vector Backbone Description: Backbone Size:5133; Vector Backbone:pTE-mcs; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:19174563
Comments: There are minor discrepancies between depositor's and Addgene's sequence. Depositor is aware of these and thinks they should not have any affect on function.
Proper citation: RRID:Addgene_20318 Copy
Species: Synthetic gene
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:5582; Vector Backbone:pSMT3-rpsA; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20352111
Proper citation: RRID:Addgene_24663 Copy
Species: Synthetic gene
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:5582; Vector Backbone:pSMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20352111
Proper citation: RRID:Addgene_24664 Copy
Genetic Insert: pKW08-Lx-Int
Vector Backbone Description: Backbone Size:8535; Vector Backbone:pMC1M; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20434484
Proper citation: RRID:Addgene_25015 Copy
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:pJDC89; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:26934495
Proper citation: RRID:Addgene_157876 Copy
Species: Synthetic
Genetic Insert: sgRNAflaB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2H; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Comments: Addgene QC identified an IS element present in the backbone sequence. The depositing lab does not believe this impacts the functional use of the plasmid but requesting scientists should validate expected behavior prior to experimentation.
Proper citation: RRID:Addgene_149561 Copy
Species: Synthetic
Genetic Insert: sgRNAmreB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2H; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149567 Copy
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