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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pGEM_Δflv3.sm Resource Report Resource Website |
RRID:Addgene_185533 | aadA gene flanked by flv3 upstream and downstream regions | Other | Spectinomycin and Streptomycin | PMID:36370921 | Backbone Marker:Promega; Backbone Size:2986; Vector Backbone:pGEM®-T easy; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin and Streptomycin | 2026-08-15 01:31:07 | 0 | ||
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pIN204 Resource Report Resource Website |
RRID:Addgene_255795 | NrdJ-1C - XtpS | Xenorhabdus nematophila ATCC 19061 | Spectinomycin and Streptomycin | This plasmid encodes the termination unit of the non-ribosomal peptide synthetase (NRPS) called xenotetrapeptide synthetase (XtpS), split at an XUTI site and fused to the C-terminal part of the split intein NrdJ-1.When co-expressed with pIN086 and pIN200, the three fragments splice into a functional NRPS that produces the non-ribosomal peptide (NRP) called xenotetrapeptide (XTP; cyclo[Leu-D-Val-Val-D-Val]; CC(C)C[C@H]1C(=O)N[C@@H](C(=O)N[C@H](C(=O)N[C@@H](C(=O)N1)C(C)C)C(C)C)C(C)C; 410.6 g/mol). For successful NRP production, the three plasmids must be co-transformed into an E. coli strain that expresses a broad-spectrum phosphopantetheinyl transferase, such as MtaA (DH10B::mtaA) or Sfp (BAP1), which is required for the post-translational activation of the T domains. Expression is controlled by an araBAD promoter, inducible with 0.2% arabinose. | Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin | 2026-08-15 01:36:21 | 0 | ||
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pIN206 Resource Report Resource Website |
RRID:Addgene_255797 | SspGyrBC - XtpS | Xenorhabdus nematophila ATCC 19061 | Spectinomycin and Streptomycin | This plasmid encodes the termination unit of the non-ribosomal peptide synthetase (NRPS) called xenotetrapeptide synthetase (XtpS), split at an XUC site and fused to the C-terminal part of the split intein SspGyrB.When co-expressed with pIN097 and pIN202, the three fragments splice into a functional NRPS that produces the non-ribosomal peptide (NRP) called xenotetrapeptide (XTP; cyclo[Leu-D-Val-Val-D-Val]; CC(C)C[C@H]1C(=O)N[C@@H](C(=O)N[C@H](C(=O)N[C@@H](C(=O)N1)C(C)C)C(C)C)C(C)C; 410.6 g/mol). For successful NRP production, the three plasmids must be co-transformed into an E. coli strain that expresses a broad-spectrum phosphopantetheinyl transferase, such as MtaA (DH10B::mtaA) or Sfp (BAP1), which is required for the post-translational activation of the T domains. Expression is controlled by an araBAD promoter, inducible with 0.2% arabinose. | Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin | 2026-08-15 01:36:17 | 0 | ||
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pSB62 - pL0_STR (pro + 5U) Resource Report Resource Website |
RRID:Addgene_123186 | Catharanthus roseus STR1 promoter and 5'UTR | Catharanthus roseus | Spectinomycin and Streptomycin | PMID:31263474 | Golden Gate (MoClo; PRO + 5U) compatible STR1 promoter from Catharanthus roseus. Can be used as a control for transactivation experiments. Confirmed activation through ORCA3 and repression through ZCT1 | Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Backbone Size:2247; Vector Backbone:pICH41295; Vector Types:Plant Expression, part for plant expression; Bacterial Resistance:Spectinomycin and Streptomycin | No BpiI and BsaI sites | 2026-08-15 01:03:51 | 0 |
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pSB142 - pL0_ORCA3 (CDS1) Resource Report Resource Website |
RRID:Addgene_123185 | ORCA3 from Catharanthus roseus | Catharanthus roseus | Spectinomycin and Streptomycin | PMID:31263474 | Golden Gate (MoClo - Weber et al., 2011), CDS1 position, Octadecanoid-Responsive Catharanthus AP2 (ORCA3, GenBank: EU072424.1); activator of the Catharanthus roseus STR1 promoter | Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Backbone Size:2247; Vector Backbone:pICH41308; Vector Types:Plant Expression, part for plant expression; Bacterial Resistance:Spectinomycin and Streptomycin | No BpiI and BsaI sites | 2026-08-15 01:03:51 | 0 |
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KFP13 Resource Report Resource Website |
RRID:Addgene_119795 | Synpcc7942_1849 | Spectinomycin and Streptomycin | PMID:30540929 | Vector Backbone:Vector for targeting integration at neutral site 1 in ATCC PCC7942 genome; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin | 2026-08-15 01:03:15 | 0 | |||
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KFP14 Resource Report Resource Website |
RRID:Addgene_119796 | Synpcc7942_1557 | Spectinomycin and Streptomycin | PMID:30540929 | Vector Backbone:Vector for targeting integration at neutral site 1 in ATCC PCC7942 genome; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin | 2026-08-15 01:03:15 | 0 | |||
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pAM5057 Resource Report Resource Website 1+ mentions |
RRID:Addgene_120085 | RSF1010 Y25F aadA PconII theophylline riboswitch F with myc-yfp reporter | Broad range of genetically tractable cyanobacteria | Spectinomycin and Streptomycin | PMID:25149516 | Vector Backbone:RSF1010Y25F-AADA; Vector Types:Other; Bacterial Resistance:Spectinomycin and Streptomycin | 2026-08-15 01:03:19 | 2 | ||
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pAM5188 Resource Report Resource Website |
RRID:Addgene_120087 | pAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS). | Synechococcus elongatus PCC 7942 | Spectinomycin and Streptomycin | PMID:27902432 | Backbone Size:6936; Vector Backbone:pAM4788; Vector Types:other; Bacterial Resistance:Spectinomycin and Streptomycin | 2026-08-15 01:03:19 | 0 | ||
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pTargetF-FRT Resource Report Resource Website |
RRID:Addgene_113637 | sgRNA-FRT | Synthetic | Spectinomycin and Streptomycin | PMID:29884781 | Vector Backbone:pTargetF; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin and Streptomycin | 2026-08-15 01:02:13 | 0 | ||
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relAE335Q_NS1 Resource Report Resource Website |
RRID:Addgene_100917 | RelA fragment from E.coli with point mutation E335Q | E. coli | Spectinomycin and Streptomycin | PMID:29241543 | Backbone Size:5700; Vector Backbone:pam1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin | E 335 converted to Q | 2026-08-15 01:00:15 | 0 | |
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P0050::kaiBC S431E T432E NS1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101836 | kaiBC S431E T432E with p0050 promoter | S. elongatus PCC7942 | Spectinomycin and Streptomycin | PMID:28430105 | Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin | S431E T432E | 2026-08-15 01:00:22 | 1 | |
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Ptrc::zwf_fbp_opcA Resource Report Resource Website 1+ mentions |
RRID:Addgene_101846 | sp/st resistance casette followed by ptrc promoter | NA | Spectinomycin and Streptomycin | PMID:28430105 | Backbone Size:4400; Vector Backbone:pBR322; Vector Types:Cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin | 2026-08-15 01:00:22 | 1 | ||
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rpaA_NS1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101829 | RpaA with its native promoter | S. elongatus PCC7942 | Spectinomycin and Streptomycin | PMID:28430105 | Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin | 2026-08-15 01:00:22 | 1 | ||
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pTrc99S-ssDsbA-TTlight-4xDQNAT Resource Report Resource Website |
RRID:Addgene_128402 | Tetanus toxin light chain domain | C. tetani | Spectinomycin and Streptomycin | PMID:33536221 | Backbone Size:4535; Vector Backbone:pTrc99S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin | Inactivating E234A mutation | 2026-08-15 01:04:46 | 0 | |
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pTrc99S-ssDsbA-EPA-DNNNS-DQNRT Resource Report Resource Website |
RRID:Addgene_128404 | Exotoxin A | P. aeruginosa | Spectinomycin and Streptomycin | PMID:33536221 | Backbone Size:4535; Vector Backbone:pTrc99S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin | 242DNNNS246 and 384DQNRT388 engineered glycosylation sites | 2026-08-15 01:04:46 | 0 | |
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pTrc99S-ssDsbA-PD-4xDQNAT Resource Report Resource Website |
RRID:Addgene_128399 | H. influenzae protein D | H. influenzae | Spectinomycin and Streptomycin | PMID:33536221 | Backbone Size:4535; Vector Backbone:pTrc99S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin | 2026-08-15 01:04:47 | 0 | ||
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pAM5411 Resource Report Resource Website |
RRID:Addgene_132663 | aadA | Other | Spectinomycin and Streptomycin | PMID:31585408 | Backbone Marker:-; Backbone Size:7000; Vector Backbone:pAM5406; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin | 2026-08-15 01:05:30 | 0 | ||
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29 pCOLI_S_C_STREP Resource Report Resource Website |
RRID:Addgene_160171 | Spectinomycin and Streptomycin | PMID:32955754 | Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid | Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin | 2026-08-15 01:08:39 | 0 | |||
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27 pCOLI_S_C_HIS Resource Report Resource Website |
RRID:Addgene_160169 | Spectinomycin and Streptomycin | PMID:32955754 | Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid | Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin | 2026-08-15 01:08:36 | 0 |
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