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Species: Other
Genetic Insert: aadA gene flanked by flv3 upstream and downstream regions
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2986; Vector Backbone:pGEM®-T easy; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:36370921
Proper citation: RRID:Addgene_185533 Copy
Species: Xenorhabdus nematophila ATCC 19061
Genetic Insert: NrdJ-1C - XtpS
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Comments: This plasmid encodes the termination unit of the non-ribosomal peptide synthetase (NRPS) called xenotetrapeptide synthetase (XtpS), split at an XUTI site and fused to the C-terminal part of the split intein NrdJ-1.When co-expressed with pIN086 and pIN200, the three fragments splice into a functional NRPS that produces the non-ribosomal peptide (NRP) called xenotetrapeptide (XTP; cyclo[Leu-D-Val-Val-D-Val]; CC(C)C[C@H]1C(=O)N[C@@H](C(=O)N[C@H](C(=O)N[C@@H](C(=O)N1)C(C)C)C(C)C)C(C)C; 410.6 g/mol).
For successful NRP production, the three plasmids must be co-transformed into an E. coli strain that expresses a broad-spectrum phosphopantetheinyl transferase, such as MtaA (DH10B::mtaA) or Sfp (BAP1), which is required for the post-translational activation of the T domains.
Expression is controlled by an araBAD promoter, inducible with 0.2% arabinose.
Proper citation: RRID:Addgene_255795 Copy
Species: Xenorhabdus nematophila ATCC 19061
Genetic Insert: SspGyrBC - XtpS
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Comments: This plasmid encodes the termination unit of the non-ribosomal peptide synthetase (NRPS) called xenotetrapeptide synthetase (XtpS), split at an XUC site and fused to the C-terminal part of the split intein SspGyrB.When co-expressed with pIN097 and pIN202, the three fragments splice into a functional NRPS that produces the non-ribosomal peptide (NRP) called xenotetrapeptide (XTP; cyclo[Leu-D-Val-Val-D-Val]; CC(C)C[C@H]1C(=O)N[C@@H](C(=O)N[C@H](C(=O)N[C@@H](C(=O)N1)C(C)C)C(C)C)C(C)C; 410.6 g/mol).
For successful NRP production, the three plasmids must be co-transformed into an E. coli strain that expresses a broad-spectrum phosphopantetheinyl transferase, such as MtaA (DH10B::mtaA) or Sfp (BAP1), which is required for the post-translational activation of the T domains.
Expression is controlled by an araBAD promoter, inducible with 0.2% arabinose.
Proper citation: RRID:Addgene_255797 Copy
Species: Catharanthus roseus
Genetic Insert: Catharanthus roseus STR1 promoter and 5'UTR
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Backbone Size:2247; Vector Backbone:pICH41295; Vector Types:Plant Expression, part for plant expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:31263474
Comments: Golden Gate (MoClo; PRO + 5U) compatible STR1 promoter from Catharanthus roseus. Can be used as a control for transactivation experiments. Confirmed activation through ORCA3 and repression through ZCT1
Proper citation: RRID:Addgene_123186 Copy
Species: Catharanthus roseus
Genetic Insert: ORCA3 from Catharanthus roseus
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Backbone Size:2247; Vector Backbone:pICH41308; Vector Types:Plant Expression, part for plant expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:31263474
Comments: Golden Gate (MoClo - Weber et al., 2011), CDS1 position, Octadecanoid-Responsive Catharanthus AP2 (ORCA3, GenBank: EU072424.1); activator of the Catharanthus roseus STR1 promoter
Proper citation: RRID:Addgene_123185 Copy
Genetic Insert: Synpcc7942_1849
Vector Backbone Description: Vector Backbone:Vector for targeting integration at neutral site 1 in ATCC PCC7942 genome; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:30540929
Proper citation: RRID:Addgene_119795 Copy
Genetic Insert: Synpcc7942_1557
Vector Backbone Description: Vector Backbone:Vector for targeting integration at neutral site 1 in ATCC PCC7942 genome; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:30540929
Proper citation: RRID:Addgene_119796 Copy
Species: Broad range of genetically tractable cyanobacteria
Genetic Insert: RSF1010 Y25F aadA PconII theophylline riboswitch F with myc-yfp reporter
Vector Backbone Description: Vector Backbone:RSF1010Y25F-AADA; Vector Types:Other; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:25149516
Proper citation: RRID:Addgene_120085 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: pAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
Vector Backbone Description: Backbone Size:6936; Vector Backbone:pAM4788; Vector Types:other; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:27902432
Proper citation: RRID:Addgene_120087 Copy
Species: Synthetic
Genetic Insert: sgRNA-FRT
Vector Backbone Description: Vector Backbone:pTargetF; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:29884781
Proper citation: RRID:Addgene_113637 Copy
Species: E. coli
Genetic Insert: RelA fragment from E.coli with point mutation E335Q
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pam1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:29241543
Proper citation: RRID:Addgene_100917 Copy
Species: S. elongatus PCC7942
Genetic Insert: kaiBC S431E T432E with p0050 promoter
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:28430105
Proper citation: RRID:Addgene_101836 Copy
Species: NA
Genetic Insert: sp/st resistance casette followed by ptrc promoter
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pBR322; Vector Types:Cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:28430105
Proper citation: RRID:Addgene_101846 Copy
Species: S. elongatus PCC7942
Genetic Insert: RpaA with its native promoter
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:28430105
Proper citation: RRID:Addgene_101829 Copy
Species: C. tetani
Genetic Insert: Tetanus toxin light chain domain
Vector Backbone Description: Backbone Size:4535; Vector Backbone:pTrc99S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:33536221
Proper citation: RRID:Addgene_128402 Copy
Species: P. aeruginosa
Genetic Insert: Exotoxin A
Vector Backbone Description: Backbone Size:4535; Vector Backbone:pTrc99S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:33536221
Proper citation: RRID:Addgene_128404 Copy
Species: H. influenzae
Genetic Insert: H. influenzae protein D
Vector Backbone Description: Backbone Size:4535; Vector Backbone:pTrc99S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:33536221
Proper citation: RRID:Addgene_128399 Copy
Species: Other
Genetic Insert: aadA
Vector Backbone Description: Backbone Marker:-; Backbone Size:7000; Vector Backbone:pAM5406; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:31585408
Proper citation: RRID:Addgene_132663 Copy
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:32955754
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid
Proper citation: RRID:Addgene_160171 Copy
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:32955754
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid
Proper citation: RRID:Addgene_160169 Copy
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