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Species: E. coli
Genetic Insert: ClbD
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114146 Copy
Species: E. coli
Genetic Insert: ClbF
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114148 Copy
Species: E. coli
Genetic Insert: ClbE
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114147 Copy
Species: E. coli
Genetic Insert: OsmC
Vector Backbone Description: Backbone Size:5386; Vector Backbone:pSKB3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15103136
Comments: Please note that the plasmid pSKB3.EG10680 contains a Kanamycin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
Proper citation: RRID:Addgene_11436 Copy
Species: E. coli
Genetic Insert: Lvl0 5'-2xTetApt-mVenus-PEST
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30327560
Proper citation: RRID:Addgene_115962 Copy
Species: E. coli
Genetic Insert: Lvl0 TetR SapI/BsaI Mut
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30327560
Proper citation: RRID:Addgene_115947 Copy
Species: E. coli
Genetic Insert: mcherry
Vector Backbone Description: Vector Backbone:pOSIP-KL; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29765036
Proper citation: RRID:Addgene_116947 Copy
Species: E. coli
Genetic Insert: b-lactamase
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2642; Vector Backbone:modified pGPS3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11602576
Comments: Positive control for mutagenesis experiments: reversion of the stop codon results in ampicillin resistance.
The pGPS3 has a deletion from BglI to BglII that destroys the ampicillin resistance gene. The ampicillin resistance gene containing the stop codon was then cloned in between AflIII and KpnI.
Please also see pGFPuv from BD Biosciences as another control for forward mutation assays. Catalog # 6079-1 http://www.clontech.com/US/Products/Fluorescent_Proteins_and_Reporters/Fluorescent_Proteins_by_Name/GFP_GFPuv_Fluorescent_Proteins?sitex=10020:22372:US .
Proper citation: RRID:Addgene_11724 Copy
Species: E. coli
Genetic Insert: MetQ (N229A)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5652; Vector Backbone:pET19b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30352853
Proper citation: RRID:Addgene_118268 Copy
Species: E. coli
Genetic Insert: MetN (N295A)
Vector Backbone Description: Backbone Marker:Thermo Scientific Invitrogen; Backbone Size:3963; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30352853
Comments: delta metNIQ E. coli strain BW2115 was a created in the Rees lab by modifying E. coli genomic DNA
Proper citation: RRID:Addgene_118258 Copy
Species: E. coli
Genetic Insert: MetN
Vector Backbone Description: Backbone Marker:Thermo Scientific Invitrogen; Backbone Size:3963; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30352853
Proper citation: RRID:Addgene_118257 Copy
Species: E. coli
Genetic Insert: MetN (N295A)
Vector Backbone Description: Backbone Marker:Thermo Scientific Invitrogen; Backbone Size:3963; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30352853
Proper citation: RRID:Addgene_118259 Copy
Species: E. coli
Genetic Insert: MetN (N295A)
Vector Backbone Description: Backbone Marker:Thermo Scientific Invitrogen; Backbone Size:3963; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30352853
Proper citation: RRID:Addgene_118256 Copy
Species: E. coli
Genetic Insert: Dam human codon optimized
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:5400; Vector Backbone:p3Flag-EYFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30982744
Proper citation: RRID:Addgene_121909 Copy
Species: E. coli
Genetic Insert: LacZ
Vector Backbone Description: Vector Backbone:pUASg; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28761084
Proper citation: RRID:Addgene_122486 Copy
Species: E. coli
Genetic Insert: self-targeting gRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33450024
Comments: Please note this plasmid contains Illumina 2nd step primer capture sites within the insert sequence that can affect next generation sequencing results. We suggest using sanger sequencing when working with this plasmid.
Proper citation: RRID:Addgene_124221 Copy
Species: E. coli
Genetic Insert: SpCas9-HF1
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33450024
Proper citation: RRID:Addgene_124226 Copy
Species: E. coli
Genetic Insert: Protein A and hyperactive Tn5 transposase (Tnp) fusion protein
Vector Backbone Description: Backbone Size:8079; Vector Backbone:pTXB1-Tn5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31036827
Comments: Please note that verifying this plasmid by sequencing or digest is challenging due to the presence of the helper plasmid, as multiple bands or mispriming is likely to occur. See Addgene's sequencing results to compare sequences.
This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but can reduce transformation or transfection efficiencies. If you have trouble isolating the monomeric version of this plasmid, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_124601 Copy
Species: E. coli
Genetic Insert: bla
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31604953
Comments: This plasmid is a derivative of repressilator plasmids presented in: Potvin-Trottier et al, 2016, Nature.
Proper citation: RRID:Addgene_127855 Copy
Species: E. coli
Genetic Insert: Maltose binding protein
Vector Backbone Description: Backbone Size:4535; Vector Backbone:pTrc99S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:33536221
Comments: Depositor confirms mutation Y342H does not affect plasmid function.
Proper citation: RRID:Addgene_128398 Copy
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