Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: E. coli
Genetic Insert: E. coli type I-E Cas5
Vector Backbone Description: Backbone Size:5221; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34985758
Comments: If used in a publication, please acknowledge that the plasmid pEcCas5 was generated by U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility. The associated work was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.
Proper citation: RRID:Addgene_170090 Copy
Species: E. coli
Genetic Insert: E. coli type I-E Cse2
Vector Backbone Description: Backbone Size:5230; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34985758
Comments: If used in a publication, please acknowledge that the plasmid pEcCse2 was generated by U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility. The associated work was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.
Proper citation: RRID:Addgene_170094 Copy
Species: E. coli
Genetic Insert: Maltose-binding protein (MalE)
Vector Backbone Description: Backbone Marker:IBA GmbH; Backbone Size:3286; Vector Backbone:pASK-IBA2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30600549
Comments: Spycatcher was published in Zakeri et al. (2012), PNAS 109, ppE690-7
Proper citation: RRID:Addgene_107422 Copy
Species: E. coli
Genetic Insert: etpBAC locus
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4094; Vector Backbone:pBAD/myc-His A; Vector Types:Bacterial Expression, n/a; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16552055
Comments: Please note that the His and Myc tags are not in frame with the ORF. In addition, there is a E65G mutation in EtpB. Depositing lab does not think this mutation will affect plasmid function.
Proper citation: RRID:Addgene_32999 Copy
Species: E. coli
Genetic Insert: LacO (256 copies)
Vector Backbone Description: Vector Backbone:Ylplac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18614049
Comments: Addgene cannot verify or guarantee the presence of all 256 copies of LacO. This element is unstable.
For use in two-plasmid-based gene-nuclear periphery tethering assay with either plasmids pAK67 (Addgene #33142).
pVS2 (Addgene #33144) contains 256 LacO repeats and a GAL-GFP-GALpA-reporter gene (pVS1 is identical but does not contain the reporter gene). The reporter was previously characterized and contains a GFP open reading frame flanked by the GAL1 promoter and GAL1 3′ UTR ([Abruzzi et al., 2006] and [Dower et al., 2004]).
pKA67 expresses both LacI-GFP and Nup49-GFP to mark the first plasmid as well as the nuclear periphery with GFP.
Proper citation: RRID:Addgene_33143 Copy
Species: E. coli
Genetic Insert: Improved MBP based Maltose Biosensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2300; Vector Backbone:pRSET-A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21989929
Comments: The GFP-T203V mutation decreases the fluorescence emission of the apo state and increases that of the saturated state in GCaMP2. In the context of MBP165-cpGFP.PPYF, the T203V mutation decreases the fluorescence emission of the apo state by half, whereas saturated fluorescence and affinity are unchanged, increasing ΔF/F to 6.5.
Proper citation: RRID:Addgene_33373 Copy
Species: E. Coli
Genetic Insert: LplA W37V
Vector Backbone Description: Backbone Marker:Gift from John Cronan; Backbone Size:4800; Vector Backbone:pYFJ16; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20534555
Proper citation: RRID:Addgene_34838 Copy
Species: E. coli
Genetic Insert: Escherichia coli RNase E residues 1-400
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:7280; Vector Backbone:pTYB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16854990
Proper citation: RRID:Addgene_17949 Copy
Species: E. coli
Genetic Insert: [sd2] T7 RNAP-TCC linker-degron
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35379961
Proper citation: RRID:Addgene_179679 Copy
Species: E. coli
Genetic Insert: FLIPglu F16F Delta13
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5603; Vector Backbone:pEF/myc/ER; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20354141
Proper citation: RRID:Addgene_18022 Copy
Species: E. coli
Genetic Insert: FLIPglu F16A Delta13
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20354141
Proper citation: RRID:Addgene_18014 Copy
Species: E. coli
Genetic Insert: FLIPglu D154A Delta13
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20354141
Proper citation: RRID:Addgene_18015 Copy
Species: E. coli
Genetic Insert: pLac promoter
Vector Backbone Description: Vector Backbone:pSEVA331; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34767345
Comments: Read the preprint at https://www.biorxiv.org/content/10.1101/2021.06.09.447691v1.full
Proper citation: RRID:Addgene_180528 Copy
Species: E. coli
Genetic Insert: LetB (a.k.a. b1834, yebT, ECK1833)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5523; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_181818 Copy
Species: E. coli
Genetic Insert: LetB (a.k.a. b1834, yebT, ECK1833)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5523; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_181819 Copy
Species: E. coli
Genetic Insert: LetB (a.k.a. b1834, yebT, ECK1833)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5523; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_181820 Copy
Species: E. coli
Genetic Insert: mcherry
Vector Backbone Description: Vector Backbone:pOSIP-KL; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29765036
Proper citation: RRID:Addgene_116947 Copy
Species: E. coli
Genetic Insert: b-lactamase
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2642; Vector Backbone:modified pGPS3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11602576
Comments: Positive control for mutagenesis experiments: reversion of the stop codon results in ampicillin resistance.
The pGPS3 has a deletion from BglI to BglII that destroys the ampicillin resistance gene. The ampicillin resistance gene containing the stop codon was then cloned in between AflIII and KpnI.
Please also see pGFPuv from BD Biosciences as another control for forward mutation assays. Catalog # 6079-1 http://www.clontech.com/US/Products/Fluorescent_Proteins_and_Reporters/Fluorescent_Proteins_by_Name/GFP_GFPuv_Fluorescent_Proteins?sitex=10020:22372:US .
Proper citation: RRID:Addgene_11724 Copy
Species: E. coli
Genetic Insert: MetQ (N229A)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5652; Vector Backbone:pET19b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30352853
Proper citation: RRID:Addgene_118268 Copy
Species: E. coli
Genetic Insert: MetN (N295A)
Vector Backbone Description: Backbone Marker:Thermo Scientific Invitrogen; Backbone Size:3963; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30352853
Comments: delta metNIQ E. coli strain BW2115 was a created in the Rees lab by modifying E. coli genomic DNA
Proper citation: RRID:Addgene_118258 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the RRID Resources search. From here you can search through a compilation of resources used by RRID and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that RRID has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on RRID then you can log in from here to get additional features in RRID such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into RRID you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within RRID that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
