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Vector Backbone Description: Backbone Size:2893; Vector Backbone:-; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27247386
Proper citation: RRID:Addgene_78269 Copy
Genetic Insert: tse2 toxin
Vector Backbone Description: Vector Backbone:pDS132; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31201277
Proper citation: RRID:Addgene_127451 Copy
Genetic Insert: mqsR toxin
Vector Backbone Description: Vector Backbone:pDS132; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31201277
Proper citation: RRID:Addgene_127450 Copy
Genetic Insert: mqsR toxin
Vector Backbone Description: Vector Backbone:pDS132; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31201277
Proper citation: RRID:Addgene_127453 Copy
Species: Synthetic
Genetic Insert: mTurquoise2
Vector Backbone Description: Backbone Size:2200; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29564885
Comments: The insert mTq2-Tat was designed by G.Grossi and M. Schwarz-Schilling and cloned by A. Dupin and M. Schwarz-Schilling. mTq2 sequence is from Goedhart, J. et al. Nat. Commun. 2012, 3, 751. Tat sequence is from Bayer, T. S. et al. RNA 2005, 11, 1848−57. The peptide linker sequence between between YPet and PCP is TRGGGGS.
Proper citation: RRID:Addgene_127635 Copy
Vector Backbone Description: Vector Backbone:pEMY11; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29753070
Comments: Promoter characterization cassette for promoter expression strength when integrated into ChrXV of S. cerevisiae.
Proper citation: RRID:Addgene_127714 Copy
Species: Synthetic
Genetic Insert: Synthetic CRISPR array containing four identical T. thermophilus spacers
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3920; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31278272
Proper citation: RRID:Addgene_127764 Copy
Species: Homo sapiens
Genetic Insert: A3A-W98Y
Vector Backbone Description: Vector Backbone:pBT195; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31332326
Comments: Along with pBT195, this plasmid can be used to clone pBT361 by SapI or LguI restriction/ligation (Golden Gate). pBT361 is mutagenic in bacteria and care must be taken during cloning, handling and propagation. Benchling map: https://benchling.com/s/seq-viWaBa6R2LjXykdEZnZo
Proper citation: RRID:Addgene_127846 Copy
Genetic Insert: PSP1 target (GGTT PAM)
Vector Backbone Description: Vector Backbone:pACYC; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31171706
Proper citation: RRID:Addgene_127926 Copy
Species: From Rhodococcus sp, codon optimized for E. coli
Genetic Insert: CocE
Vector Backbone Description: Vector Backbone:pSB4C5 (iGEM registry); Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31462649
Proper citation: RRID:Addgene_128129 Copy
Species: Other
Genetic Insert: Piq - mRFP, parS
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31406375
Comments: Cloning method: Golden Gate.
Proper citation: RRID:Addgene_128294 Copy
Species: Other
Genetic Insert: Piq - mRFP, BBa_J23100 - phlF, parS
Vector Backbone Description: Vector Backbone:pMB1+rop; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31406375
Comments: Cloning method: Golden Gate.
Please note: Plasmid contains a T180A mutation in mRFP. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_128285 Copy
Species: Other
Genetic Insert: Piq - mRFP, parS
Vector Backbone Description: Vector Backbone:pMB1+rop; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31406375
Comments: Cloning method: Golden Gate.
Proper citation: RRID:Addgene_128280 Copy
Species: Synthetic
Genetic Insert: Fluoride riboswitch-regulated 3-way junction dimeric broccoli
Vector Backbone Description: Backbone Size:2204; Vector Backbone:Synthetic; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31829623
Proper citation: RRID:Addgene_128811 Copy
Species: E. coli
Genetic Insert: rpoZ
Vector Backbone Description: Backbone Size:3939; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23389035
Proper citation: RRID:Addgene_128837 Copy
Species: Synthetic
Genetic Insert: pSB1C3(BsmBI-)-BsaI-mCherry*(27-218)-BsaI
Vector Backbone Description: Backbone Marker:Baojun Wang's Lab; Vector Backbone:pSB1C3(BsmBI-); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33850130
Proper citation: RRID:Addgene_161945 Copy
Species: Synthetic
Genetic Insert: pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
Vector Backbone Description: Backbone Marker:The SEVA Commonwealth; Vector Backbone:pSEVA321; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33850130
Proper citation: RRID:Addgene_161954 Copy
Vector Backbone Description: Backbone Size:6696; Vector Backbone:pMSR0; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33247281
Comments: Please note Addgene NGS result identified a mismatch in pBP1_RepA. Depositor has not confirmed if this is a concern for plasmid function. We recommend additional QC to confirm plasmid is functional.
Proper citation: RRID:Addgene_161984 Copy
Vector Backbone Description: Backbone Size:5624; Vector Backbone:pMSR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33247281
Proper citation: RRID:Addgene_161983 Copy
Species: Synthetic
Genetic Insert: N-terminal tag, HiBiT-MBP-[Factor Xa cleavage site]
Vector Backbone Description: Vector Backbone:pTwist; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34693026
Proper citation: RRID:Addgene_162296 Copy
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