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Species: Synthetic
Genetic Insert: GalS-L
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25035932
Comments: GalS-L and FruR-L also described as LLhS_Q54A and LLhF_Q60S respectively in
PLoS One. 2013 Dec 30;8(12):e83502. doi: 10.1371/journal.pone.0083502. eCollection 2013.
Rheostats and toggle switches for modulating protein function.
Meinhardt S, Manley MW Jr, Parente DJ, Swint-Kruse L.
Proper citation: RRID:Addgene_60764 Copy
Species: Synthetic
Genetic Insert: GalS-L
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25035932
Comments: GalS-L and RbsR-L also described as LLhS_Q54A and LLhR_Q60A respectively in
PLoS One. 2013 Dec 30;8(12):e83502. doi: 10.1371/journal.pone.0083502. eCollection 2013.
Rheostats and toggle switches for modulating protein function.
Meinhardt S, Manley MW Jr, Parente DJ, Swint-Kruse L.
Proper citation: RRID:Addgene_60763 Copy
Species: Synthetic
Genetic Insert: FruR-L
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25035932
Comments: FruR-L and RbsR-L also described as LLhF_Q60S and LLhR_Q60A respectively in
PLoS One. 2013 Dec 30;8(12):e83502. doi: 10.1371/journal.pone.0083502. eCollection 2013.
Rheostats and toggle switches for modulating protein function.
Meinhardt S, Manley MW Jr, Parente DJ, Swint-Kruse L.
Proper citation: RRID:Addgene_60761 Copy
Species: Synthetic
Genetic Insert: GalS-L
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25035932
Comments: GalS-L and TreR-L also described as LLhS_Q54A and TreR_V52A respectively in
PLoS One. 2013 Dec 30;8(12):e83502. doi: 10.1371/journal.pone.0083502. eCollection 2013.
Rheostats and toggle switches for modulating protein function.
Meinhardt S, Manley MW Jr, Parente DJ, Swint-Kruse L.
Proper citation: RRID:Addgene_60765 Copy
Species: Synthetic
Genetic Insert: GalS-T
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25035932
Proper citation: RRID:Addgene_60775 Copy
Species: Synthetic
Genetic Insert: FruR-T
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25035932
Proper citation: RRID:Addgene_60772 Copy
Species: Synthetic
Genetic Insert: RbsR-L
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25035932
Proper citation: RRID:Addgene_60776 Copy
Species: Synthetic
Genetic Insert: 25x BoxB
Vector Backbone Description: Backbone Marker:Dr. David Russell, UTSW; Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24035393
Comments: the authors of Gehring et al. Mol Cell. 2003 Apr;11(4):939-49 were contacted by us and granted their permission to distribute the 25xboxb construct generated in our lab from their precursor for non-profit purpose via Addgene
Proper citation: RRID:Addgene_60817 Copy
Species: Synthetic
Genetic Insert: LANS4
Vector Backbone Description: Backbone Size:5943; Vector Backbone:pTriEx; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26083500
Proper citation: RRID:Addgene_60785 Copy
Species: Synthetic
Genetic Insert: GFPγ
Vector Backbone Description: Backbone Size:4021; Vector Backbone:pFA6a; Vector Types:Yeast Integrating; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25612242
Proper citation: RRID:Addgene_60781 Copy
Species: Synthetic
Genetic Insert: Residues 1-179 of split T7 RNAP
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pBAD33; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23479654
Proper citation: RRID:Addgene_60728 Copy
Species: Synthetic
Genetic Insert: Residues 1-179 of split T7 RNAP
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pBAD33; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23479654
Proper citation: RRID:Addgene_60727 Copy
Species: Synthetic
Genetic Insert: Residues 1-179 of split T7 RNAP
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pBAD33; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23479654
Proper citation: RRID:Addgene_60730 Copy
Species: Synthetic
Genetic Insert: GalS-L
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:4000; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25035932
Comments: GalS-L also described as LLhS_Q54A in
PLoS One. 2013 Dec 30;8(12):e83502. doi: 10.1371/journal.pone.0083502. eCollection 2013.
Rheostats and toggle switches for modulating protein function.
Meinhardt S, Manley MW Jr, Parente DJ, Swint-Kruse L.
Proper citation: RRID:Addgene_60743 Copy
Species: Synthetic
Genetic Insert: single polypeptide FPX biosensor for caspase-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25622108
Proper citation: RRID:Addgene_60885 Copy
Species: Synthetic
Genetic Insert: single polypeptide FPX biosensor for caspase-3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25622108
Proper citation: RRID:Addgene_60883 Copy
Species: Synthetic
Genetic Insert: PIP2 dimerization dependent sensor RA+B monomers
Vector Backbone Description: Vector Backbone:pUB2.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25622108
Comments: Contains a dimerization dependent fluorescent protein, originally described by Alford, S.C., Ding, Y., Simmen, T., and Campbell, R.E. (2012). Dimerization-Dependent Green and Yellow Fluorescent Proteins. ACS Synth. Biol. 1, 569–575.
Addgene NGS results identified a frameshift mutation in the HygR gene originally indicated in this plasmid. The depositing lab has confirmed that their experiments were done with transient transfection and did not make use of Hygromycin selection. For users who need a mammalian selection marker, they recommend moving the biosensor coding region from this plasmid into a different plasmid backbone.
Proper citation: RRID:Addgene_60937 Copy
Species: Synthetic
Genetic Insert: PKA FPX Sensor
Vector Backbone Description: Vector Backbone:pUB2.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25622108
Comments: Contains a dimerization dependent fluorescent protein, originally described by Alford, S.C., Ding, Y., Simmen, T., and Campbell, R.E. (2012). Dimerization-Dependent Green and Yellow Fluorescent Proteins. ACS Synth. Biol. 1, 569–575.
Proper citation: RRID:Addgene_60936 Copy
Species: Synthetic
Genetic Insert: SpCas9
Vector Backbone Description: Backbone Size:2905; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25326897
Proper citation: RRID:Addgene_60957 Copy
Species: Synthetic
Genetic Insert: GFP-Luciferase fusion
Vector Backbone Description: Backbone Size:2877; Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24755823
Proper citation: RRID:Addgene_60976 Copy
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