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Species: E.coli
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23878244
Comments: The PconNoHindM2 promoter is the same as PconNoHind except for a substitution mutation at the −10 site that modestly increases transcription.
Proper citation: RRID:Addgene_53037 Copy
Species: E.coli
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23878244
Comments: The PconNoHindM12 promoter is the same as PconNoHind except for two substitution mutations at the −10 and −35 sites that moderately decrease transcription.
Proper citation: RRID:Addgene_53036 Copy
Species: E.coli
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23878244
Proper citation: RRID:Addgene_53041 Copy
Species: Homo sapiens
Genetic Insert: Potassium voltage-gated channel subfamily KQT member 1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8900283
Proper citation: RRID:Addgene_53048 Copy
Species: Homo sapiens
Genetic Insert: Potassium voltage-gated channel subfamily E member 1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8900283
Proper citation: RRID:Addgene_53049 Copy
Species: Homo sapiens
Genetic Insert: Potassium voltage-gated channel subfamily H member 2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11005845
Proper citation: RRID:Addgene_53051 Copy
Species: Homo sapiens
Genetic Insert: Potassium voltage-gated channel subfamily E member 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10185; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8900283
Proper citation: RRID:Addgene_53050 Copy
Species: Homo sapiens
Genetic Insert: Potassium voltage-gated channel subfamily H member 2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9882738
Proper citation: RRID:Addgene_53057 Copy
Species: Homo sapiens
Genetic Insert: Potassium voltage-gated channel subfamily H member 2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9547387
Proper citation: RRID:Addgene_53058 Copy
Species: Homo sapiens
Genetic Insert: Potassium voltage-gated channel subfamily H member 2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11005845
Proper citation: RRID:Addgene_53055 Copy
Species: Homo sapiens
Genetic Insert: Potassium voltage-gated channel subfamily H member 2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11005845
Proper citation: RRID:Addgene_53054 Copy
Species: wheat
Genetic Insert: wheat U6 promoter - gRNA
Vector Backbone Description: Backbone Marker:CWBIO; Backbone Size:2700; Vector Backbone:pUC-T; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23929338
Proper citation: RRID:Addgene_53062 Copy
Species: Streptococcus pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:pGREEN; Backbone Size:3741; Vector Backbone:pJIT163; Vector Types:Plant expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23929338
Proper citation: RRID:Addgene_53064 Copy
Species: Drosophila melanogaster
Genetic Insert: 5HT1B
Vector Backbone Description: Vector Backbone:pME18ST; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23817146
Comments: Note that this plasmid has a few differences from the Entrez Genbank reference sequence for Drosophila 5-HT1B. It was cloned from transgenic fly that has UAS-d5-HT1B (Bloomington #27632) and can be considered a functional variant.
Plasmid can be directly used for expression in HEK cells. Insert cloned into the NotI and XhoI sites. Has a vertebrate Kozak sequence (GCCACC) immediately upstream of the ATG.
Proper citation: RRID:Addgene_53108 Copy
Species: Hepatitis B virus
Genetic Insert: HBSP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6360; Vector Backbone:pBIND; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24758376
Proper citation: RRID:Addgene_53113 Copy
Species: Drosophila melanogaster
Genetic Insert: 5HT7
Vector Backbone Description: Vector Backbone:pME18ST; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23817146
Comments: Note that this plasmid has a few differences from the Entrez Genbank reference sequence for Drosophila 5-HT7. It was cloned from a w1118 fly strain and can be considered a functional variant.
Plasmid can be directly used for expression in HEK cells. Insert cloned into the NotI and XhoI sites. Has a vertebrate Kozak sequence (GCCACC) immediately upstream of the ATG.
Proper citation: RRID:Addgene_53111 Copy
Species: Homo sapiens
Genetic Insert: OCT1
Vector Backbone Description: Vector Backbone:pLenti-CMV-BSD; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24717434
Proper citation: RRID:Addgene_53118 Copy
Species: Staphylococcus aureus
Genetic Insert: theophylline riboswitch and alpha hemolysin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5442; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24874202
Proper citation: RRID:Addgene_53116 Copy
Species: Homo sapiens
Genetic Insert: Gfa2
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8120611
Comments: The plasmid is a pUC18 vector containing the human GFAP sequences from -2163 to +47 (the human gfa2 segment), with the initiating ATG mutated to TTG, placed in front of the nuclear targeted E. coli lacZ gene, which in turn is followed by a fragment of the mouse protamine-1 gene. The latter supplies an intron, stabilizing 3' UTR, and a polyadenylation signal. If cloning a cDNA, it is advisable to retain the mP-1 segment. The lacZ gene can be excised by digestion with BamHI, and replaced with your gene of interest. If cloning a genomic sequence that includes an intron and polyadenylation site (this may compromise astrocyte specificity--see Su et al. 2004), the mP-1 region can also be excised, or alternatively, a number of sites flank the gfa2 segment allowing its isolation.
Restriction sites that may be useful are as follows:
EcoRI: flanks the gfa2, lacZ, and mP-1 segments
Bgl II: 5' flank of gfa2 & 3' flank of mP-1
Bam HI: flanks the lacZ gene
Sal I: cuts uniquely between the gfa2 promoter and the LacZ gene
Sph I and Hind III: cut uniquely just 3' of the mP-1 segment
Although the GFAP promoter appears to be the best choice for targeting transgene expression to astrocytes in mice, please be aware that neuronal expression has also occasionally been observed (Su et al. 2004).
Proper citation: RRID:Addgene_53126 Copy
Species: Aphelenchus avenae
Genetic Insert: AavLEA1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5708; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25120007
Proper citation: RRID:Addgene_53093 Copy
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