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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pHL1757 Resource Report Resource Website |
RRID:Addgene_53037 | GFP | E.coli | Ampicillin | PMID:23878244 | The PconNoHindM2 promoter is the same as PconNoHind except for a substitution mutation at the −10 site that modestly increases transcription. | Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:27 | 0 | |
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pHL1756 Resource Report Resource Website 1+ mentions |
RRID:Addgene_53036 | GFP | E.coli | Ampicillin | PMID:23878244 | The PconNoHindM12 promoter is the same as PconNoHind except for two substitution mutations at the −10 and −35 sites that moderately decrease transcription. | Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:31 | 1 | |
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pHL1855 Resource Report Resource Website |
RRID:Addgene_53041 | GFP | E.coli | Ampicillin | PMID:23878244 | Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:27 | 0 | ||
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pSP64-hKCNQ1 Resource Report Resource Website |
RRID:Addgene_53048 | Potassium voltage-gated channel subfamily KQT member 1 | Homo sapiens | Ampicillin | PMID:8900283 | Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:27 | 0 | ||
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pSP64-hKCNE1 Resource Report Resource Website |
RRID:Addgene_53049 | Potassium voltage-gated channel subfamily E member 1 | Homo sapiens | Ampicillin | PMID:8900283 | Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:31 | 0 | ||
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pSP64-hERG1a Resource Report Resource Website 1+ mentions |
RRID:Addgene_53051 | Potassium voltage-gated channel subfamily H member 2 | Homo sapiens | Ampicillin | PMID:11005845 | Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:27 | 2 | ||
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pCEP4-hKCNE1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_53050 | Potassium voltage-gated channel subfamily E member 1 | Homo sapiens | Ampicillin | PMID:8900283 | Backbone Marker:Invitrogen; Backbone Size:10185; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:27 | 1 | ||
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pSP64-hERG1a D540K Resource Report Resource Website |
RRID:Addgene_53057 | Potassium voltage-gated channel subfamily H member 2 | Homo sapiens | Ampicillin | PMID:9882738 | Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin | changed Aspartate 540 to Lysine | 2026-08-15 01:16:27 | 0 | |
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pSP64-hERG1a S631A Resource Report Resource Website |
RRID:Addgene_53058 | Potassium voltage-gated channel subfamily H member 2 | Homo sapiens | Ampicillin | PMID:9547387 | Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin | changed Serine 631 to Alanine | 2026-08-15 01:16:31 | 0 | |
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pSP64-hERG1a T623A Resource Report Resource Website 1+ mentions |
RRID:Addgene_53055 | Potassium voltage-gated channel subfamily H member 2 | Homo sapiens | Ampicillin | PMID:11005845 | Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin | changed Threonine 623 to Alanine | 2026-08-15 01:16:31 | 1 | |
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pSP64-hERG1a F656A Resource Report Resource Website 1+ mentions |
RRID:Addgene_53054 | Potassium voltage-gated channel subfamily H member 2 | Homo sapiens | Ampicillin | PMID:11005845 | Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin | changed Phenylalanine 656 to Alanine | 2026-08-15 01:16:27 | 1 | |
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pU6-gRNA Resource Report Resource Website 1+ mentions |
RRID:Addgene_53062 | wheat U6 promoter - gRNA | wheat | Ampicillin | PMID:23929338 | Backbone Marker:CWBIO; Backbone Size:2700; Vector Backbone:pUC-T; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:27 | 4 | ||
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pJIT163-2NLSCas9 Resource Report Resource Website 1+ mentions |
RRID:Addgene_53064 | Cas9 | Streptococcus pyogenes | Ampicillin | PMID:23929338 | Backbone Marker:pGREEN; Backbone Size:3741; Vector Backbone:pJIT163; Vector Types:Plant expression; Bacterial Resistance:Ampicillin | Rice codon optimized | 2026-08-15 01:16:31 | 3 | |
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pME18ST-d5-HT1B Resource Report Resource Website |
RRID:Addgene_53108 | 5HT1B | Drosophila melanogaster | Ampicillin | PMID:23817146 | Note that this plasmid has a few differences from the Entrez Genbank reference sequence for Drosophila 5-HT1B. It was cloned from transgenic fly that has UAS-d5-HT1B (Bloomington #27632) and can be considered a functional variant. Plasmid can be directly used for expression in HEK cells. Insert cloned into the NotI and XhoI sites. Has a vertebrate Kozak sequence (GCCACC) immediately upstream of the ATG. | Vector Backbone:pME18ST; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:27 | 0 | |
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pBIND-HBSP Resource Report Resource Website 1+ mentions |
RRID:Addgene_53113 | HBSP | Hepatitis B virus | Ampicillin | PMID:24758376 | Backbone Marker:Promega; Backbone Size:6360; Vector Backbone:pBIND; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:32 | 1 | ||
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pME18ST-d5-HT7 Resource Report Resource Website |
RRID:Addgene_53111 | 5HT7 | Drosophila melanogaster | Ampicillin | PMID:23817146 | Note that this plasmid has a few differences from the Entrez Genbank reference sequence for Drosophila 5-HT7. It was cloned from a w1118 fly strain and can be considered a functional variant. Plasmid can be directly used for expression in HEK cells. Insert cloned into the NotI and XhoI sites. Has a vertebrate Kozak sequence (GCCACC) immediately upstream of the ATG. | Vector Backbone:pME18ST; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:28 | 0 | |
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pLenti-OC-IRES-BSD Resource Report Resource Website |
RRID:Addgene_53118 | OCT1 | Homo sapiens | Ampicillin | PMID:24717434 | Vector Backbone:pLenti-CMV-BSD; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:28 | 0 | ||
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JF001A Resource Report Resource Website |
RRID:Addgene_53116 | theophylline riboswitch and alpha hemolysin | Staphylococcus aureus | Ampicillin | PMID:24874202 | Backbone Marker:Novagen; Backbone Size:5442; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:28 | 0 | ||
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pGfa2-nLac Resource Report Resource Website 1+ mentions |
RRID:Addgene_53126 | Gfa2 | Homo sapiens | Ampicillin | PMID:8120611 | The plasmid is a pUC18 vector containing the human GFAP sequences from -2163 to +47 (the human gfa2 segment), with the initiating ATG mutated to TTG, placed in front of the nuclear targeted E. coli lacZ gene, which in turn is followed by a fragment of the mouse protamine-1 gene. The latter supplies an intron, stabilizing 3' UTR, and a polyadenylation signal. If cloning a cDNA, it is advisable to retain the mP-1 segment. The lacZ gene can be excised by digestion with BamHI, and replaced with your gene of interest. If cloning a genomic sequence that includes an intron and polyadenylation site (this may compromise astrocyte specificity--see Su et al. 2004), the mP-1 region can also be excised, or alternatively, a number of sites flank the gfa2 segment allowing its isolation. Restriction sites that may be useful are as follows: EcoRI: flanks the gfa2, lacZ, and mP-1 segments Bgl II: 5' flank of gfa2 & 3' flank of mP-1 Bam HI: flanks the lacZ gene Sal I: cuts uniquely between the gfa2 promoter and the LacZ gene Sph I and Hind III: cut uniquely just 3' of the mP-1 segment Although the GFAP promoter appears to be the best choice for targeting transgene expression to astrocytes in mice, please be aware that neuronal expression has also occasionally been observed (Su et al. 2004). | Vector Backbone:pUC18; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin | contains -2163 to +47 (the human gfa2 segment), with the initiating ATG mutated to TTG | 2026-08-15 01:16:28 | 5 |
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pET15b-AavLEA1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_53093 | AavLEA1 | Aphelenchus avenae | Ampicillin | PMID:25120007 | Backbone Marker:Novagen; Backbone Size:5708; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:27 | 4 |
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