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Species: Arabidopsis thaliana
Genetic Insert: SPL10
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBIB-KAN-GW; Vector Types:used to create transgenic lines; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21123653
Comments: Created by cloning 4.9-kb (including 1.8 kb upstream of CDS, CDS, and 1.6 kb downstream from CDS) genomic fragment in pENTR/D-TOPO (Invitrogen). This plasmid was recombined with pBIB-KAN-GW using LR clonase (Invitrogen).
Proper citation: RRID:Addgene_27407 Copy
Species: Arabidopsis thaliana
Genetic Insert: SPL10 mutant
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBIB-KAN-GW; Vector Types:used to create transgenic lines; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21123653
Comments: Created by cloning 4.9-kb (including 1.8 kb upstream of CDS, CDS, and 1.6 kb downstream from CDS) genomic fragment in pENTR/D-TOPO (Invitrogen). The Quick-
Change Lightning Site-Directed Mutagenesis kit (Stratagene) was used on the resulting plasmids to disrupt miR156-binding sites (primers 5'-CCATGAATCTCATGATTTCTACAGTGCACTGTCATTGCTCTCAACTACTTCGGATTCACAAGGGATC-3' and 5'-GATCCCTTGTGAATCCGAAGTAGTTGAGAGCAATGACAGTGCACTGTAGAAATCATGAGATTCATGG-3').
This plasmid was recombined with pBIB-KAN-GW using LR clonase (Invitrogen).
Proper citation: RRID:Addgene_27408 Copy
Species: Arabidopsis thaliana
Genetic Insert: SPL11
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBIB-KAN-GW; Vector Types:used to create transgenic lines; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21123653
Comments: Created by cloning 5.5-kb (including 2.7 kb upstream of
CDS, CDS, and 1.3 kb downstream from CDS) genomic fragment in pENTR/D-TOPO (Invitrogen). This plasmid was recombined with pBIB-KAN-GW using LR clonase (Invitrogen).
Proper citation: RRID:Addgene_27398 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIB1 (1-171aa)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8000; Vector Backbone:pGADT7rec; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21037589
Proper citation: RRID:Addgene_28246 Copy
Species: Arabidopsis thaliana
Genetic Insert: cry2
Vector Backbone Description: Backbone Size:7480; Vector Backbone:na; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21037589
Proper citation: RRID:Addgene_28244 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIB1(deltaNLS)-pmGFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21037589
Proper citation: RRID:Addgene_28240 Copy
Species: Arabidopsis thaliana
Genetic Insert: p!SV40-PakGBD-mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4864; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19749742
Proper citation: RRID:Addgene_22280 Copy
Species: Arabidopsis thaliana
Genetic Insert: p!SV40-mCherry-WaspGBD
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5216; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19749742
Proper citation: RRID:Addgene_22281 Copy
Species: Arabidopsis thaliana
Genetic Insert: PIF6APB
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4860; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19749742
Proper citation: RRID:Addgene_22276 Copy
Species: Arabidopsis thaliana
Genetic Insert: IntersectinDHPH-20aaLinker-mYFP-PIF6APB
Vector Backbone Description: Backbone Size:4860; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19749742
Proper citation: RRID:Addgene_22278 Copy
Species: Arabidopsis thaliana
Genetic Insert: A. thaliana tRNA ligase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5255; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20075163
Comments: We have found that this protein expresses best in the BL21 (DE3) RIPL cells from Stratagene. Standard BL21 (DE3) strains that lack tRNAs for rare codons do not work well (or at all).
Proper citation: RRID:Addgene_32242 Copy
Species: Arabidopsis thaliana
Genetic Insert: ASK1
Vector Backbone Description: Backbone Size:0; Vector Backbone:pFB-HTB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20927106
Proper citation: RRID:Addgene_29517 Copy
Species: Arabidopsis thaliana
Genetic Insert: COI1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:0; Vector Backbone:pFastBac-GTE; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20927106
Proper citation: RRID:Addgene_29516 Copy
Species: Arabidopsis thaliana
Genetic Insert: Auxin Signaling Receptor AFB2
Vector Backbone Description: Vector Backbone:pGP8GF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32123041
Proper citation: RRID:Addgene_188426 Copy
Species: Arabidopsis thaliana
Genetic Insert: Auxin Signaling Receptor TIR1
Vector Backbone Description: Vector Backbone:pGP8GF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32123041
Proper citation: RRID:Addgene_188427 Copy
Species: Arabidopsis thaliana
Genetic Insert: sgRNA cassette under U6 promoter
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:2974; Vector Backbone:pJet1.2; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35022409
Comments: This vector was designed and constructed by Dr. Tomasz Bieluszewski.
Detailed protocol for CRISPR/Cas9-based mutagenesis using this system in:
Bieluszewski T, Szymanska-Lejman M, Dziegielewski W, Zhu L, Ziolkowski PA. Efficient Generation of CRISPR/Cas9-Based Mutants Supported by Fluorescent Seed Selection in Different Arabidopsis Accessions, in: Plant Gametogenesis: Methods and Protocols (ed. Christophe Lambing), Methods in Molecular Biology, vol. 2484.
https://doi.org/10.1007/978-1-0716-2253-7_13
https://pubmed.ncbi.nlm.nih.gov/35461452/
Proper citation: RRID:Addgene_173157 Copy
Species: Arabidopsis thaliana
Genetic Insert: SulR
Vector Backbone Description: Backbone Marker:made by Tomáš Moravec, IEB, Prague, Czech Republic (Addgene #106207); Backbone Size:2604; Vector Backbone:pDB1alpha11:empty; Vector Types:Plant Expression, Synthetic Biology, binary vector for Escherichia coli and Agrobacterium tumefaciens-mediated plant transformation; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36609768
Proper citation: RRID:Addgene_186424 Copy
Species: Arabidopsis thaliana
Genetic Insert: SulR
Vector Backbone Description: Backbone Marker:GoldenBraid kit from Diego Orzaez (Addgene kit # 1000000076 ), IBMCP, Valencia, Spain; Backbone Size:2106; Vector Backbone:pUPD2; Vector Types:Plant Expression, Synthetic Biology, CDS (part B3-B4-B5), GoldenBraid level 0; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36609768
Proper citation: RRID:Addgene_186423 Copy
Species: Arabidopsis thaliana
Genetic Insert: KanR
Vector Backbone Description: Backbone Marker:GoldenBraid 2.0 kit from Diego Orzaez (Addgene kit # 1000000076 ), IBMCP, Valencia, Spain; Backbone Size:6702; Vector Backbone:pDGB3_omega1 (Plasmid #68238); Vector Types:Plant Expression, Synthetic Biology, binary vector for Escherichia coli and Agrobacterium tumefaciens-mediated plant transformation; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36609768
Proper citation: RRID:Addgene_186426 Copy
Species: Arabidopsis thaliana
Genetic Insert: SulR
Vector Backbone Description: Backbone Marker:GoldenBraid 2.0 kit from Diego Orzaez (Addgene kit # 1000000076 ), IBMCP, Valencia, Spain; Backbone Size:2605; Vector Backbone:pDGB1_alpha2 (Plasmid #68225); Vector Types:Plant Expression, Synthetic Biology, binary vector for Escherichia coli and Agrobacterium tumefaciens-mediated plant transformation; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36609768
Proper citation: RRID:Addgene_186425 Copy
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